Spheroplast fusion and heterokaryon formation in Mucor racemosus.

abstract：:A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could...

journal_title：Journal of bacteriology

authors： Ray PH,Benedict CD

更新日期：1980-04-01 00:00:00

abstract：:Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenin...

journal_title：Journal of bacteriology

authors： Mudd JB,Lee IM,Liu HY,Calavan EC

更新日期：1979-02-01 00:00:00

abstract：:Pore-forming colicins are soluble bacteriocins which form voltage-gated ion channels in the inner membrane of Escherichia coli. To reach their target, these colicins first bind to a receptor located on the outer membrane and then are translocated through the envelope. Colicins are subdivided into two groups according ...

journal_title：Journal of bacteriology

authors： Duché D,Letellier L,Géli V,Bénédetti H,Baty D

更新日期：1995-09-01 00:00:00

abstract：:Sites of transposon Tn7 insertion in the Escherichia coli chromosome were examined, and two distinct classes of target sites differing in nucleotide sequence were identified. The target site choice was found to be determined by Tn7-encoded transposition genes. ...

journal_title：Journal of bacteriology

authors： Kubo KM,Craig NL

更新日期：1990-05-01 00:00:00

abstract：:Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...

journal_title：Journal of bacteriology

authors： Hook-Barnard I,Johnson XB,Hinton DM

更新日期：2006-12-01 00:00:00

abstract：:Streptomyces azureus carried one copy or less of plasmid pSA1, which elicited pocks at 0.1 to 1.0%. Strain PK100 was isolated from the wild-type strain after UV irradiation. PK100 carried approximately 20 to 30 copies of pSA1.1, a derivative of pSA1. Plasmid pSA1.1 elicited pocks at 100% and inhibited spore and thiost...

journal_title：Journal of bacteriology

authors： Miyoshi YK,Ogata S,Hayashida S

更新日期：1986-10-01 00:00:00

abstract：:Campylobacter jejuni commensally colonizes the cecum of birds. The RacR (reduced ability to colonize) response regulator was previously shown to be important in avian colonization. To explore the means by which RacR and its cognate sensor kinase RacS may modulate C. jejuni physiology and colonization, ΔracR and ΔracS ...

journal_title：Journal of bacteriology

authors： Apel D,Ellermeier J,Pryjma M,Dirita VJ,Gaynor EC

更新日期：2012-05-01 00:00:00

abstract：:The reactivation of membranes from Gaffkya homari for the synthesis of sodium dodecyl sulfate-insoluble peptidoglycan (SDS-insoluble PG) was achieved by successive cycles of freeze-thawing (- 196 versus 25 degrees C). The presence of G. homari walls during this process affected the synthesis of both SDS-soluble (nasce...

journal_title：Journal of bacteriology

authors： Kalomiris E,Bardin C,Neuhaus FC

更新日期：1982-05-01 00:00:00

abstract：:Mevalonate (MVA) metabolism provides the isoprenoids used in archaeal lipid biosynthesis. In synthesis of isopentenyl diphosphate, the classical MVA pathway involves decarboxylation of mevalonate diphosphate, while an alternate pathway has been proposed to involve decarboxylation of mevalonate monophosphate. To identi...

journal_title：Journal of bacteriology

authors： Vannice JC,Skaff DA,Keightley A,Addo JK,Wyckoff GJ,Miziorko HM

更新日期：2014-03-01 00:00:00

abstract：:The alpha-aminoadipate pathway for lysine biosynthesis is present only in fungi. The alpha-aminoadipate reductase (AAR) of this pathway catalyzes the conversion of alpha-aminoadipic acid to alpha-aminoadipic-delta-semialdehyde by a complex mechanism involving two gene products, Lys2p and Lys5p. The LYS2 and LYS5 genes...

journal_title：Journal of bacteriology

authors： Guo S,Evans SA,Wilkes MB,Bhattacharjee JK

更新日期：2001-12-01 00:00:00

abstract：:Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...

journal_title：Journal of bacteriology

authors： Summers RG,Wendt-Pienkowski E,Motamedi H,Hutchinson CR

更新日期：1992-03-01 00:00:00

abstract：:Several characteristics of Mycobacterium tuberculosis (e.g., conserved genome and low growth rate) have severely restricted the study of the microorganism. The discovery of IS6110 raised hopes of overcoming these obstacles. However, our knowledge of this IS element is relatively limited; even its two basic characteris...

journal_title：Journal of bacteriology

authors： Fang Z,Kenna DT,Doig C,Smittipat DN,Palittapongarnpim P,Watt B,Forbes KJ

更新日期：2001-09-01 00:00:00

abstract：:Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I co...

journal_title：Journal of bacteriology

authors： van den Broek D,Chin-A-Woeng TF,Bloemberg GV,Lugtenberg BJ

更新日期：2005-01-01 00:00:00

abstract：:Nocardia brasiliensis possess proteolytic activities that can be readily detected in a variety of media. In a modified formulation of a growth medium originally used for Streptomyces aureofaciens, N. brasiliensis was found to secrete proteolytic enzymes, one of which was capable of hydrolyzing casein. This enzyme was ...

journal_title：Journal of bacteriology

authors： Zlotnik H,Schramm VL,Buckley HR

更新日期：1984-02-01 00:00:00

abstract：:The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...

journal_title：Journal of bacteriology

authors： Bedford DJ,Schweizer E,Hopwood DA,Khosla C

更新日期：1995-08-01 00:00:00

abstract：:The peptidoglycan cell wall determines the shape and structural integrity of a bacterial cell. Class B penicillin-binding proteins (PBPs) carry a transpeptidase activity that cross-links peptidoglycan strands via their peptide side chains, and some of these proteins are directly involved in cell shape determination. N...

journal_title：Journal of bacteriology

authors： Wei Y,Havasy T,McPherson DC,Popham DL

更新日期：2003-08-01 00:00:00

abstract：:A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...

journal_title：Journal of bacteriology

authors： Rumbak E,Rawlings DE,Lindsey GG,Woods DR

更新日期：1991-11-01 00:00:00

abstract：:Lysyl-transfer ribonucleic acid synthetase (EC 6.1.1.6) was identified as four polypeptide spots after two-dimensional polyacrylamide gel electrophoresis of whole-cell lysates of Escherichia coli. Identification was made by migration with partially purified enzyme preparations, by peptide map patterns, by mutant analy...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bloch PL,Van Bogelen RA,Neidhardt FC

更新日期：1981-04-01 00:00:00

abstract：:DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

journal_title：Journal of bacteriology

authors： Asai T,Kogoma T

更新日期：1994-11-01 00:00:00

abstract：:Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

journal_title：Journal of bacteriology

authors： Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

更新日期：2011-10-01 00:00:00

abstract：:We report the first study of tRNA modification in psychrotolerant archaea, specifically in the archaeon Methanococcoides burtonii grown at 4 and 23 degrees C. For comparison, unfractionated tRNA from the archaeal hyperthermophile Stetteria hydrogenophila cultured at 93 degrees C was examined. Analysis of modified nucl...

journal_title：Journal of bacteriology

authors： Noon KR,Guymon R,Crain PF,McCloskey JA,Thomm M,Lim J,Cavicchioli R

更新日期：2003-09-01 00:00:00

abstract：:Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...

journal_title：Journal of bacteriology

authors： Solbiati JO,Ciaccio M,Farías RN,Salomón RA

更新日期：1996-06-01 00:00:00

abstract：:Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) from Acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the Escherichia coli B enzyme. The molecular weights of the two native active enzymes as well as their alpha and beta...

journal_title：Journal of bacteriology

authors： Kleppe RK,Kleppe K

更新日期：1976-02-01 00:00:00

abstract：:The S-layers of Thermus thermophilus HB27 and T. thermophilus HB8 are composed of protein units of 95 kDa (P95) and 100 kDa (P100), respectively. We have selected S-layer deletion mutants from both strains by complete replacement of the slpA gene. Mutants of the two strains showed similar defects in growth and morphol...

journal_title：Journal of bacteriology

authors： Fernández-Herrero LA,Olabarría G,Castón JR,Lasa I,Berenguer J

更新日期：1995-10-01 00:00:00

abstract：:Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

journal_title：Journal of bacteriology

authors： Friedberg I,Avigad G

更新日期：1968-08-01 00:00:00

abstract：:Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...

journal_title：Journal of bacteriology

authors： Rottem S,Markowitz O

更新日期：1979-12-01 00:00:00

abstract：:Chorismate mutase from Streptomyces aureofaciens was purified 12-fold. This enzyme preparation did not show any activity when tested for anthranilate synthetase, prephenate dehydrogenase, or prephenate dehydratase. The catalytic activity of chorismate mutase has a broad optimum between pH 7 and 8. The initial velocity...

journal_title：Journal of bacteriology

authors： Görisch H,Lingens F

更新日期：1973-05-01 00:00:00

abstract：:Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...

journal_title：Journal of bacteriology

authors： Hemmi H,Takahashi Y,Shibuya K,Nakayama T,Nishino T

更新日期：2005-03-01 00:00:00

abstract：:In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...

journal_title：Journal of bacteriology

authors： Hsieh FC,Chen CT,Weng YT,Peng SS,Chen YC,Huang LY,Hu HT,Wu YL,Lin NC,Wu WF

更新日期：2011-10-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...

journal_title：Journal of bacteriology

authors： Houot L,Chang S,Pickering BS,Absalon C,Watnick PI

更新日期：2010-06-01 00:00:00