Preparations and properties of ribonucleic acid polymerase from Acinetobacter calcoaceticus.

Abstract:

:Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) from Acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the Escherichia coli B enzyme. The molecular weights of the two native active enzymes as well as their alpha and beta subunits appeared to be similar. No subunit corresponding to that of sigma from E. coli was found, and furthermore no separation between the beta subunits could be detected by gel electrophoresis. A number of different DNAs were transcribed by the enzyme from A. calcoaceticus. Maximal RNA synthesis occurred at pH 8.7, 10 mM Mg2+, or 0.3 mM Mn2+ and at a total ionic strength of 0.1. Higher ionic strengths led to increasing inhibition of transcription and at mu = 0.4 complete inhibition was observed. The mechanism of inhibition of salt was not related to the initiation event as observed with T4 core RNA polymerase (R.Kleppe, 1975). In an attempt to understand the mechanism of inhibition by salt, the effect of ionic strength on the sedimentation properties of the enzyme was investigated. At low ionic strength, enzyme species with sedimentation coefficients, s20,w, of 5.8S, 12.4S, and 19.3S were present. In buffers with higher ionic strengths the relative amounts of the 12.4S species decreased. It is suggested, therefore, that the inhibition of activity at higher salt concentrations is caused by a decrease in concentration of the active enzyme species.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kleppe RK,Kleppe K

doi

10.1128/JB.125.2.435-443.1976

keywords:

subject

Has Abstract

pub_date

1976-02-01 00:00:00

pages

435-43

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

125

pub_type

杂志文章
  • A genetic analysis of Xis and FIS interactions with their binding sites in bacteriophage lambda.

    abstract::The bacteriophage P22-based challenge-phage system was used to study the binding of Xis and FIS to their sites in attP of bacteriophage lambda. Challenge phages were constructed that contained the X1, X2, and F sites within the P22 Pant promoter, which is required for expression of antirepressor. If Xis and FIS bind t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.5954-5963.1991

    authors: Numrych TE,Gumport RI,Gardner JF

    更新日期:1991-10-01 00:00:00

  • Effect of bacteriophage lambda infection on synthesis of groE protein and other Escherichia coli proteins.

    abstract::We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1050-1063.1982

    authors: Drahos DJ,Hendrix RW

    更新日期:1982-03-01 00:00:00

  • Differential localization of Mre proteins with PBP2 in Rhodobacter sphaeroides.

    abstract::In Rhodobacter sphaeroides, MreB, MreC, MreD, PBP2, and RodA are encoded at the same locus. The localizations of PBP2, MreB, and MreC, which have all been implicated in the synthesis of the peptidoglycan layer, were investigated under different growth conditions to gain insight into the relationships between these pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.5.1691-1700.2006

    authors: Slovak PM,Porter SL,Armitage JP

    更新日期:2006-03-01 00:00:00

  • REDUCTION OF SELENITE BY INTACT YEAST CELLS AND CELL-FREE PREPARATIONS.

    abstract::Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754-762. 1963.-Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.4.754-762.1963

    authors: FALCONE G,NICKERSON WJ

    更新日期:1963-04-01 00:00:00

  • uhp-directed, glucose 6-phosphate membrane receptor in Escherichia coli.

    abstract::Membrane vesicles were characterized for their ability to specifically bind [14C]glucose 6-phosphate. Membranes prepared from a strain carrying a ColE1 uhp hybrid plasmid showed significantly enhanced glucose 6-phosphate binding. It is hypothesized that glucose 6-phosphate binding to these membranes is due to a uhpR-d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.347-349.1980

    authors: Goldenbaum PE,Farmer KS

    更新日期:1980-04-01 00:00:00

  • Mobilization of Haemophilus influenzae chromosomal markers by an Escherichia coli F' factor.

    abstract::Filter matings between E. coli K-12 strains carrying an F'::Tn5,Tn9 factor with H. influenzae Rd strains gave rise to kanamycin-chloramphenicol-resistant H. influenzae strains at a frequency of approximately 10(-6). Transfer of the F' factor to H. influenzae was verified by expression of unselected markers in H. influ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.1905-1910.1987

    authors: Deich RA,Green BA

    更新日期:1987-05-01 00:00:00

  • Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942.

    abstract::An open reading frame (slr0899) on the genome of Synechocystis sp. strain PCC 6803 encodes a polypeptide of 149 amino acid residues, the sequence of which is 40% identical to that of cyanase from Escherichia coli. Introduction into a cyanase-deficient E. coli strain of a plasmid-borne slr0899 resulted in expression of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.18.5744-5750.1997

    authors: Harano Y,Suzuki I,Maeda S,Kaneko T,Tabata S,Omata T

    更新日期:1997-09-01 00:00:00

  • Genetic characterization of a novel rhizobial plasmid conjugation system in Rhizobium leguminosarum bv. viciae strain VF39SM.

    abstract::Rhizobium leguminosarum strain VF39SM contains two plasmids that have previously been shown to be self-transmissible by conjugation. One of these plasmids, pRleVF39b, is shown in this study to carry a set of plasmid transfer genes that differs significantly from conjugation systems previously studied in the rhizobia b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01234-12

    authors: Ding H,Yip CB,Hynes MF

    更新日期:2013-01-01 00:00:00

  • Outer membrane of gram-negative bacteria. XVIII. Electron microscopic studies on porin insertion sites and growth of cell surface of Salmonella typhimurium.

    abstract::Salmonella typhimurium contains three "major proteins" or "porins" (34K, 35K, and 36K) in the outer membrane. A mutant strain producing only the 35K porin was first grown in media containing high concentrations of NaCl to "repress" the porin synthesis and then was shifted into a medium without NaCl. The newly made por...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.2.687-702.1978

    authors: Smit J,Nikaido H

    更新日期:1978-08-01 00:00:00

  • Effect of an oxygen-tolerant bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex from Clostridium difficile on butyrate production in Escherichia coli.

    abstract::The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00321-13

    authors: Aboulnaga el-H,Pinkenburg O,Schiffels J,El-Refai A,Buckel W,Selmer T

    更新日期:2013-08-01 00:00:00

  • A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.

    abstract::The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05990-11

    authors: Smaldone GT,Revelles O,Gaballa A,Sauer U,Antelmann H,Helmann JD

    更新日期:2012-05-01 00:00:00

  • Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12.

    abstract::To isolate genes from Escherichia coli which regulate the labile hydrogenase activity, a plasmid library was used to transform hydL mutants lacking the labile hydrogenase. A single type of gene, designated hydG, was isolated. This gene also partially restored the hydrogenase activity in hydF mutants (which are defecti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4448-4456.1989

    authors: Stoker K,Reijnders WN,Oltmann LF,Stouthamer AH

    更新日期:1989-08-01 00:00:00

  • Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei.

    abstract::Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.4.925-930.1966

    authors: Birnbaum J,Lichstein HC

    更新日期:1966-10-01 00:00:00

  • Disruption of aldA influences the developmental process in Myxococcus xanthus.

    abstract::Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.2.546-550.2000

    authors: Ward MJ,Lew H,Zusman DR

    更新日期:2000-01-01 00:00:00

  • Expression of the Vibrio cholerae gene encoding aldehyde dehydrogenase is under control of ToxR, the cholera toxin transcriptional activator.

    abstract::The toxR gene of Vibrio cholerae encodes a transcriptional activator required for the expression of the cholera toxin genes (ctxAB) and more than 15 other genes encoding secreted or membrane proteins. The latter group includes virulence genes involved in the biogenesis of the TCP pilus, the accessory colonization fact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.9.2842-2851.1991

    authors: Parsot C,Mekalanos JJ

    更新日期:1991-05-01 00:00:00

  • CTX prophages in classical biotype Vibrio cholerae: functional phage genes but dysfunctional phage genomes.

    abstract::CTXphi is a filamentous, lysogenic bacteriophage whose genome encodes cholera toxin, the primary virulence factor produced by Vibrio cholerae. CTX prophages in O1 El Tor and O139 strains of V. cholerae are found within arrays of genetically related elements integrated at a single locus within the V. cholerae large chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.24.6992-6998.2000

    authors: Davis BM,Moyer KE,Boyd EF,Waldor MK

    更新日期:2000-12-01 00:00:00

  • Isolation and characterization of a gene from Streptomyces sp. strain C5 that confers the ability to convert daunomycin to doxorubicin on Streptomyces lividans TK24.

    abstract::DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3389-3395.1996

    authors: Dickens ML,Strohl WR

    更新日期:1996-06-01 00:00:00

  • Cross-linkage and cross-linking of peptidoglycan in Escherichia coli: definition, determination, and implications.

    abstract::The glycan chains in peptidoglycan or murein are cross-linked by transpeptidation of the peptide side chains. To assess the fraction of side chains involved in cross-bridges, distinction has been made between cross-linkage and cross-linking. The first expression refers to the situation in unlabeled (or fully labeled) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.6.2028-2031.1992

    authors: Driehuis F,de Jonge B,Nanninga N

    更新日期:1992-03-01 00:00:00

  • Intramacrophage Infection Reinforces the Virulence of Edwardsiella tarda.

    abstract:UNLABELLED:Edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. However, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. Here, we show that E. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00978-15

    authors: Zhang L,Ni C,Xu W,Dai T,Yang D,Wang Q,Zhang Y,Liu Q

    更新日期:2016-04-28 00:00:00

  • Studies with a gluconate-6-phosphate dehydrogenase mutant in Escherichia coli strain C.

    abstract::A map location of the gluconate-6-phosphate dehydrogenase (gnd) marker was estimated in Escherichia coli C at approximately 46 min by P1 transduction. The gnd locus appears to lie between the co-transducible histidine and prophage P2 location I markers. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.1056-1058.1973

    authors: Robbins RS,Kelly B

    更新日期:1973-11-01 00:00:00

  • Divergence among genes encoding the elongation factor Tu of Yersinia Species.

    abstract::Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01067-08

    authors: Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

    更新日期:2008-11-01 00:00:00

  • ATPase activity and oligomeric state of TrwK, the VirB4 homologue of the plasmid R388 type IV secretion system.

    abstract::Type IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. VirB4 was suggested to be an ATPase involved in energizing pilus assembly and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00321-08

    authors: Arechaga I,Peña A,Zunzunegui S,del Carmen Fernández-Alonso M,Rivas G,de la Cruz F

    更新日期:2008-08-01 00:00:00

  • Chromosome and cell wall segregation in Streptococcus faecium ATCC 9790.

    abstract::Segregation was studied by measuring the positions of autoradiographic grain clusters in chains formed from single cells containing on average less than one radiolabeled chromosome strand. The degree to which chromosomal and cell wall material cosegregated was quantified by using the methods of S. Cooper and M. Weinbe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.349-352.1989

    authors: Higgins ML,Glaser D,Dicker DT,Zito ET

    更新日期:1989-01-01 00:00:00

  • Effect of light on several metabolites of carbohydrate metabolism in Phycomyces blakesleeanus.

    abstract::The concentrations of all metabolites studied, except fructose 1,6-bisphosphate from wild-type Phycomyces blakesleeanus, were light dependent. This photoregulation appears to be independent of the mad gene product(s) and also independent of carotene biosynthesis regulation. However, the photoregulation of glyceraldehy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.904-907.1987

    authors: Rua J,Rodriguez-Aparicio LB,Busto F,Soler J

    更新日期:1987-02-01 00:00:00

  • Cloning and characterization of the Pseudomonas aeruginosa zwf gene encoding glucose-6-phosphate dehydrogenase, an enzyme important in resistance to methyl viologen (paraquat).

    abstract::In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1741-1749.1998

    authors: Ma JF,Hager PW,Howell ML,Phibbs PV,Hassett DJ

    更新日期:1998-04-01 00:00:00

  • Penicillin acyltransferase in Penicillium chrysogenum.

    abstract::Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.5.1502-1508.1967

    authors: Pruess DL,Johnson MJ

    更新日期:1967-11-01 00:00:00

  • Complete genome sequence of the type strain Cupriavidus necator N-1.

    abstract::Here we announce the complete genome sequence of the copper-resistant bacterium Cupriavidus necator N-1, the type strain of the genus Cupriavidus. The genome consists of two chromosomes and two circular plasmids. Based on genome comparison, the chromosomes of C. necator N-1 share a high degree of similarity with the t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05660-11

    authors: Poehlein A,Kusian B,Friedrich B,Daniel R,Bowien B

    更新日期:2011-09-01 00:00:00

  • Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System.

    abstract::GspB is a serine-rich glycoprotein adhesin of Streptococcus gordonii that is exported to the bacterial surface by the accessory Sec system. This dedicated export pathway is comprised of seven components (SecA2, SecY2, and five accessory Sec proteins [Asp1 to Asp5]). The latter proteins have no known homologs beyond th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00057-11

    authors: Yen YT,Seepersaud R,Bensing BA,Sullam PM

    更新日期:2011-07-01 00:00:00

  • Genomic analysis of Rickettsia japonica strain YHT.

    abstract::Rickettsia japonica strain YH, isolated in 1984 in Japan, is the type strain of R. japonica, the tick-borne agent of Japanese spotted fever. Here, we report the 1.33-Mb genome of this rickettsial species. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01928-12

    authors: Dong X,El Karkouri K,Robert C,Raoult D,Fournier PE

    更新日期:2012-12-01 00:00:00

  • Regulation of Bacteriodes fragilis katB mRNA by oxidative stress and carbon limitation.

    abstract::Regulation of the katB catalase gene in the anaerobic bacterium Bacteroides fragilis was studied. Northern blot hybridization analyses revealed that katB was transcribed as an approximately 1.6-kb monocistronic mRNA. The levels of katB mRNA increased > 15-fold when anaerobic, mid-logarithmic-phase cultures were expose...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7033-7039.1997

    authors: Rocha ER,Smith CJ

    更新日期:1997-11-01 00:00:00