Abstract:
:Filter matings between E. coli K-12 strains carrying an F'::Tn5,Tn9 factor with H. influenzae Rd strains gave rise to kanamycin-chloramphenicol-resistant H. influenzae strains at a frequency of approximately 10(-6). Transfer of the F' factor to H. influenzae was verified by expression of unselected markers in H. influenzae (lac+ or cotransfer of the nonselected antibiotic resistance), physical presence of a high-molecular-weight plasmid in recipient H. influenzae cells, and detection by Southern hybridization analysis of DNA sequences specific for the F' factor replication and partition functions in recipient H. influenzae cells. H. influenzae (F' Tn5,Tn9) strains were capable of transferring kanamycin and chloramphenicol resistances to other H. influenzae strains and were capable of mobilizing H. influenzae chromosomal markers at a low frequency. Insertion of a Tn5 element in the H. influenzae genome near the novobiocin resistance gene increased the frequency of transfer of novobiocin resistance about 30-fold. Transfer of other chromosomal markers also increased, although to a lesser extent, and ordered transfer of chromosomal markers could be demonstrated. Gene transfer was insensitive to DNase I, and transfer of chromosomal (but not F' factor) markers was dependent on the H. influenzae rec-1 and rec-2 gene functions.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Deich RA,Green BAdoi
10.1128/jb.169.5.1905-1910.1987subject
Has Abstractpub_date
1987-05-01 00:00:00pages
1905-10issue
5eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::A gene coding for thermophilic beta-amylase of Clostridium thermosulfurogenes was cloned into Bacillus subtilis, and its nucleotide sequence was determined. The nucleotide sequence suggested that the thermophilic beta-amylase is translated from monocistronic mRNA as a secretory precursor with a signal peptide of 32 am...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5848-5854.1988
更新日期:1988-12-01 00:00:00
abstract::In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00674-10
更新日期:2010-11-01 00:00:00
abstract::A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.725-730.1970
更新日期:1970-03-01 00:00:00
abstract::We have cloned a DNA fragment from a genomic library of Myxococcus xanthus using an oligonucleotide probe representing conserved regions of biotin carboxylase subunits of acetyl coenzyme A (acetyl-CoA) carboxylases. The fragment contained two open reading frames (ORF1 and ORF2), designated the accB and accA genes, cap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.19.5462-5469.2000
更新日期:2000-10-01 00:00:00
abstract::Saccharomyces cerevisiae was grown in a continuous culture at a single dilution rate with input ammonia concentrations whose effects ranged from nitrogen limitation to nitrogen excess and glucose limitation. The rate of ammonia assimilation (in millimoles per gram of cells per hour) was approximately constant. Increas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.22.6672-6675.1995
更新日期:1995-11-01 00:00:00
abstract::Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.310-316.1997
更新日期:1997-01-01 00:00:00
abstract::The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2165-2170.1968
更新日期:1968-06-01 00:00:00
abstract::We isolated pili from Escherichia coli SS142. These pili had a diameter of 6 nm and an average length of 400 nm. They were composed of subunits with a molecular weight of 18,000. Their amino acid composition was determined; methionine and proline were not detected. The isolated pili retained mannose-resistant hemagglu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.1038-1044.1983
更新日期:1983-02-01 00:00:00
abstract::A circular genetic map of Bacillus stearothermophilus NUB36 was constructed by transduction with bacteriophage TP-42C and protoplast fusion. Sixty-four genes were tentatively assigned a cognate Bacillus subtilis gene based on growth response to intermediates or end products of metabolism, cross-feeding, accumulation o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.793-801.1990
更新日期:1990-02-01 00:00:00
abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1287-1293.1962
更新日期:1962-06-01 00:00:00
abstract::Pneumocystis carinii remains an important opportunistic fungal pathogen causing life-threatening pneumonia in patients with AIDS and malignancy. Currently, little is known about how the organism adapts to environmental stresses and maintains its cellular integrity. We recently discovered an open reading frame approxim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.23.6740-6745.2001
更新日期:2001-12-01 00:00:00
abstract::The DNA binding protein WhiA is conserved in Gram-positive bacteria and is present in the genetically simple cell wall-lacking mycoplasmas. The protein shows homology to eukaryotic homing endonucleases but lacks nuclease activity. WhiA was first characterized in streptomycetes, where it regulates the expression of key...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00633-17
更新日期:2018-03-26 00:00:00
abstract::Glycerol:NAD+2-OXIDOREDUCTASE (EC 1.1.1.6) was purified to homogeneity from a mutant of Escherichia coli K12 that uses this enzyme, instead of ATP:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.1.182-187.1979
更新日期:1979-10-01 00:00:00
abstract::All Escherichia coli strains so far examined possess a chromosomally encoded nanATEK-yhcH operon for the catabolism of sialic acids. These unique nine-carbon sugars are synthesized primarily by higher eukaryotes and can be used as carbon, nitrogen, and energy sources by a variety of microbial pathogens or commensals. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.16.4806-4815.2003
更新日期:2003-08-01 00:00:00
abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3448-3452.1998
更新日期:1998-07-01 00:00:00
abstract::Harvey, R. J. (University of California, Davis) and E. B. Collins. Citrate transport system of Streptococcus diacetilactis. J. Bacteriol. 83:1005-1009. 1962.-The uptake of citrate by Streptococcus diacetilactis is mediated by a transport system that was distinguished from passive diffusion by inducibility and kinetics...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.5.1005-1009.1962
更新日期:1962-05-01 00:00:00
abstract::Cultures of isocitrate dehydrogenase-deficient (icd) mutants were overgrown by double mutants (icd glt) lacking citrate synthase activity also. The icd mutants grew more slowly than wild-type cells or the double mutants because they accumulated an inhibitory metabolite (possibly citrate). Intracellular citrate levels ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.76-83.1976
更新日期:1976-07-01 00:00:00
abstract::The metE gene of Salmonella typhimurium was cloned into plasmid pACYC184 by using a lambda gt7 -metE transducing phage as a source of metE DNA. The recombinant plasmid, designated pGS41 , carries a 12.8-kilobase-pair EcoRI insert fragment. The metE gene was subcloned from pGS41 into plasmid pBR322 on a 4.2-kilobase-pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.928-933.1984
更新日期:1984-06-01 00:00:00
abstract::The phage 21 holin, S(21), forms small membrane holes that depolarize the membrane and is designated as a pinholin, as opposed to large-hole-forming holins, like S(lambda). Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate in the evolution of canonical holin-endolysin systems. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00847-07
更新日期:2007-12-01 00:00:00
abstract::A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6732-6741.1991
更新日期:1991-11-01 00:00:00
abstract::The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase. Our previous results showed that IraD determines RpoS steady-state levels during exponential growth and mediates its st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00412-09
更新日期:2009-12-01 00:00:00
abstract::Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.100-106.1974
更新日期:1974-01-01 00:00:00
abstract::The bacteriophage T4 MotA protein is a transcriptional activator of T4-modified host RNA polymerase and is required for activation of the middle class of T4 promoters. MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box consensus sequence [(t/a)(t/a)TGCTT(t/c)A]. We report th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6133-6139.1996
更新日期:1996-11-01 00:00:00
abstract::Oxalate catabolism is conducted by phylogenetically diverse organisms, including Methylobacterium extorquens AM1. Here, we investigate the central metabolism of this alphaproteobacterium during growth on oxalate by using proteomics, mutant characterization, and (13)C-labeling experiments. Our results confirm that ener...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00288-12
更新日期:2012-06-01 00:00:00
abstract::Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2879-2881.1989
更新日期:1989-05-01 00:00:00
abstract::Mutation of the spoVB gene in Bacillus subtilis causes the production of spores containing a defective cortex and unable to acquire heat resistance. The spoVB locus is highly linked to another spo locus, spoIIIF, characterized by a single mutation (I. L. Lamont and J. Mandelstam, J. Gen. Microbiol. 130:1253-1261, 1984...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.24.7942-7949.1991
更新日期:1991-12-01 00:00:00
abstract::The bacterium Caulobacter crescentus undergoes an asymmetric cell division resulting in the formation of two different daughter cells, a motile swarmer cell and a nonmotile stalked cell. These two cell types differ in their program of gene expression, their ability to replicate DNA, and the physical properties of thei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.21.6970-6981.1993
更新日期:1993-11-01 00:00:00
abstract::The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.327-330.1992
更新日期:1992-01-01 00:00:00
abstract:UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00274-15
更新日期:2015-08-01 00:00:00
abstract::Davies, Helen C. (University of Pennsylvania, Philadelphia), Fred Karush, and Joanne H. Rudd. Effect of amino acids on steady-state growth of a group A hemolytic streptococcus. J. Bacteriol. 89:421-427. 1965.-A study has been made of amino acid utilization by a strain of type 4, group A streptococcus growing under ste...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1965-02-01 00:00:00