Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942.

abstract：:Hg2+-resistant mutants were isolated from Saccharomyces cerevisiae. Although they were very much like the parental strains in terms of colony-forming ability, they grew faster than the parental strains in the presence of sublethal doses of Hg2+. The Hg2+-resistant mutations were dominant. They were centromere linked a...

journal_title：Journal of bacteriology

authors： Ono B,Ohue H,Ishihara F

更新日期：1988-12-01 00:00:00

abstract：:Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.) and Joseph Greenberg. Resistance and cross resistance of Escherichia coli S mutants to the radiomimetic agent nitrofurazone. J. Bacteriol. 85:1208-1216. 1963.-Cross-resistance relationships are described for 73 mutants of Escherichia coli s...

journal_title：Journal of bacteriology

authors： WOODY-KARRER P,GREENBERG J

更新日期：1963-06-01 00:00:00

abstract：:Pseudomonas sp. strain LB400 grows on biphenyl as the sole carbon and energy source. This organism also cooxidizes several chlorinated biphenyl congeners. Biphenyl dioxygenase activity in cell extract required addition of NAD(P)H as an electron donor for the conversion of biphenyl to cis-2,3-dihydroxy-2,3-dihydrobiphe...

journal_title：Journal of bacteriology

authors： Haddock JD,Nadim LM,Gibson DT

更新日期：1993-01-01 00:00:00

abstract：:Bacteriocins are ribosomally synthesized proteinaceous antibacterial peptides. They selectively interfere with the growth of other bacteria. The production and secretion of bacteriocins confer a distinct ecological advantage to the producer in competing against other bacteria that are present in the same ecological ni...

journal_title：Journal of bacteriology

authors： Dufour D,Barbour A,Chan Y,Cheng M,Rahman T,Thorburn M,Stewart C,Finer Y,Gong SG,Lévesque CM

更新日期：2020-05-27 00:00:00

abstract：:We isolated several new mutator mutations of the Escherichia coli replicative polymerase dnaE subunit alpha and used them and a previously reported dnaE mutation to study spontaneous frameshift and base substitution mutations. Two of these dnaE strains produce many more mutants when grown on rich (Luria-Bertani) than ...

journal_title：Journal of bacteriology

authors： Strauss BS,Roberts R,Francis L,Pouryazdanparast P

更新日期：2000-12-01 00:00:00

abstract：:Crude cell extracts of Bacillus subtilis 168T exhibit enzyme activity capable of releasing free uracil from phage PBS1 deoxyribonucleic acid (DNA) in the presence of ethylenediaminetetraacetate. By measuring the enzyme activity in 300 clones that emanated from mutagenized cells, we obtained a mutant strain that did no...

journal_title：Journal of bacteriology

authors：

更新日期：1977-08-01 00:00:00

abstract：:Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...

journal_title：Journal of bacteriology

authors： Smith RV,Evans MC

更新日期：1971-03-01 00:00:00

abstract：:A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

journal_title：Journal of bacteriology

authors： Hausman SZ,London J

更新日期：1987-04-01 00:00:00

abstract：:A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...

journal_title：Journal of bacteriology

authors： Malki S,Saimmaime I,De Luca G,Rousset M,Dermoun Z,Belaich JP

更新日期：1995-05-01 00:00:00

abstract：:In Escherichia coli cultures maximally inhibited with chloramphenicol, formation of polypeptides still continued at a slow, constant rate for at least 90 min. The rate of leucine incorporation was reduced to 0.5%, but methionine was only reduced to 2%, suggesting that chains are normally initiated with methionine but ...

journal_title：Journal of bacteriology

authors： Cremer K,Silengo L,Schlessinger D

更新日期：1974-05-01 00:00:00

abstract：:H-NS is an abundant nucleoid-associated protein involved in the maintenance of chromosomal architecture in bacteria. H-NS also has a role in silencing the expression of a variety of environmentally regulated genes during growth under nonpermissive conditions. In this study we demonstrate a role for H-NS in the negativ...

journal_title：Journal of bacteriology

authors： Nye MB,Pfau JD,Skorupski K,Taylor RK

更新日期：2000-08-01 00:00:00

abstract：:The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene produ...

journal_title：Journal of bacteriology

authors： Griffin TJ 4th,Kolodner RD

更新日期：1990-11-01 00:00:00

abstract：:Partially purified inactive glucose dehydrogenase obtained from spores which were heated at 87 or 90 C for 30 min is converted to an active from by the addition of ethylenediaminetetraacetic acid, dipicolinic acid, or some salts. The molecular weight of the inactive glucose dehydrogenase in the heated spores is about ...

journal_title：Journal of bacteriology

authors： Hachisuka Y,Tochikubo K

更新日期：1971-08-01 00:00:00

abstract：:Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the...

journal_title：Journal of bacteriology

authors： Sardesai AA,Gowrishankar J

更新日期：2001-06-01 00:00:00

abstract：:Several phosphate-starvation-inducible (psi) genetic loci in Salmonella typhimurium were identified by fusing the lacZ gene to psi promoters by using the Mu d1 and Mu d1-8 bacteriophages. Although several different starvation conditions were examined, the psi loci responded solely to phosphate deprivation. A regulator...

journal_title：Journal of bacteriology

authors： Foster JW,Spector MP

更新日期：1986-05-01 00:00:00

abstract：:The secondary structures of the eubacterial RNase P RNAs are being elucidated by a phylogenetic comparative approach. Sequences of genes encoding RNase P RNA from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. These sequences allow the refinement, to ne...

journal_title：Journal of bacteriology

authors： Brown JW,Haas ES,James BD,Hunt DA,Liu JS,Pace NR

更新日期：1991-06-01 00:00:00

abstract：:Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutant...

journal_title：Journal of bacteriology

authors： Rock CO,Park HW,Jackowski S

更新日期：2003-06-01 00:00:00

abstract：:The broad-host-range plasmid RK2 is capable of replication and stable maintenance within a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation protein TrfA, which binds to the host initiation protein, DnaA, at the plasmid origin of replication (oriV). There are two versions of ...

journal_title：Journal of bacteriology

authors： Kongsuwan K,Josh P,Picault MJ,Wijffels G,Dalrymple B

更新日期：2006-08-01 00:00:00

abstract：:The poly-beta-hydroxybutyrate (PHB) biosynthetic pathway from Alcaligenes eutrophus H16 has been cloned and expressed in Escherichia coli. Initially, an A. eutrophus H16 genomic library was constructed by using cosmid pVK102, and cosmid clones that encoded the PHB biosynthetic pathway were sought by assaying for the f...

journal_title：Journal of bacteriology

authors： Slater SC,Voige WH,Dennis DE

更新日期：1988-10-01 00:00:00

abstract：:Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...

journal_title：Journal of bacteriology

authors： Xu D,Yang C,Kaplan HB

更新日期：1998-12-01 00:00:00

abstract：:We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extra...

journal_title：Journal of bacteriology

authors： Calvin NM,Hanawalt PC

更新日期：1988-06-01 00:00:00

abstract：:Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

journal_title：Journal of bacteriology

authors： Kuroda MI,Henner D,Yanofsky C

更新日期：1988-07-01 00:00:00

abstract：:At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI...

journal_title：Journal of bacteriology

authors： Dion P,Mandelstam J

更新日期：1980-02-01 00:00:00

abstract：:The ompA gene of Escherichia coli codes for a major protein of the outer membrane. When this gene was moved between various unrelated strains (E. coli K-12 and two clinical isolates of E. coli) by transduction, the gene was expressed very poorly. Recombinants carrying "foreign" genes produced no OmpA protein which cou...

journal_title：Journal of bacteriology

authors： Beher M,Pugsley A,Schnaitman C

更新日期：1980-07-01 00:00:00

abstract：:The tail structures of bacteriophages infecting gram-positive bacteria are largely unexplored, although the phage tail mediates the initial interaction with the host cell. The temperate Lactococcus lactis phage TP901-1 of the Siphoviridae family has a long noncontractile tail with a distal baseplate. In the present st...

journal_title：Journal of bacteriology

authors： Vegge CS,Brøndsted L,Neve H,Mc Grath S,van Sinderen D,Vogensen FK

更新日期：2005-06-01 00:00:00

abstract：:The decline in colony-forming ability observed during tryptophan starvation of Bacillus subtilis auxotrophs is a concentration-dependent phenomenon. It does not manifest itself when the initial cell concentration is 10(6) cells/ml or lower. This property has been used to test the killing activity of different fraction...

journal_title：Journal of bacteriology

authors： Barlati S,Majerfeld I

更新日期：1970-02-01 00:00:00

abstract：:Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with ...

journal_title：Journal of bacteriology

authors： Wehr CT,Waskell L,Glaser DA

更新日期：1975-01-01 00:00:00

abstract：:The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...

journal_title：Journal of bacteriology

authors： Pihl TD,Sharma S,Reeve JN

更新日期：1994-10-01 00:00:00

abstract：:The genetics of antibiotic resistance in mutant strains of Streptococcus pyrogenes was studied. Utilizing a type 6 strain (9440) primarily resistant to strepttomycin (Strr), classes of mutant strains were isolated that were resistant to one of the following antibiotics: rifampin (Rifr), erythromycin (Eryr), thiostrept...

journal_title：Journal of bacteriology

authors： Stuart JG,Ferretti JJ

更新日期：1978-02-01 00:00:00

abstract：:The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...

journal_title：Journal of bacteriology

authors： Pieper-Fürst U,Madkour MH,Mayer F,Steinbüchel A

更新日期：1994-07-01 00:00:00