Role of cell wall in Saccharomyces cerevisiae mutants resistant to Hg2+.

abstract：:The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...

journal_title：Journal of bacteriology

authors： Padhy RN,Hottat FG,Coene MM,Hoet PP

更新日期：1988-04-01 00:00:00

abstract：:Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...

journal_title：Journal of bacteriology

authors： Hamrick TS,Harris SL,Spears PA,Havell EA,Horton JR,Russell PW,Orndorff PE

更新日期：2000-07-01 00:00:00

abstract：:Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription factor sigma(B), resulted in a biofilm-negative phenotype and loss of salt-induced biofilm production. Complementation of t...

journal_title：Journal of bacteriology

authors： Rachid S,Ohlsen K,Wallner U,Hacker J,Hecker M,Ziebuhr W

更新日期：2000-12-01 00:00:00

abstract：:A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

journal_title：Journal of bacteriology

authors： Wirth R,An FY,Clewell DB

更新日期：1986-03-01 00:00:00

abstract：:The genes coding for two structurally different isocitrate dehydrogenase isozymes (IDH-I and IDH-II) of a psychrophilic bacterium, Vibrio sp. strain ABE-1, were cloned and sequenced. Open reading frames of the genes (icdI and icdII) are 1,248 and 2,229 bp in length, respectively. The amino acid sequences predicted fro...

journal_title：Journal of bacteriology

authors： Ishii A,Suzuki M,Sahara T,Takada Y,Sasaki S,Fukunaga N

更新日期：1993-11-01 00:00:00

abstract：:Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked cop...

journal_title：Journal of bacteriology

authors： Waidner B,Melchers K,Stähler FN,Kist M,Bereswill S

更新日期：2005-07-01 00:00:00

abstract：:The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...

journal_title：Journal of bacteriology

authors： Hommes NG,Sayavedra-Soto LA,Arp DJ

更新日期：2001-02-01 00:00:00

abstract：:Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chrom...

journal_title：Journal of bacteriology

authors： Kanoh S,Kohlhage H,Siegert R

更新日期：1968-09-01 00:00:00

abstract：:Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Iwashima A,Nose Y

更新日期：1972-12-01 00:00:00

abstract：:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...

journal_title：Journal of bacteriology

authors： Eisenberg RC,Dobrogosz WJ

更新日期：1967-03-01 00:00:00

abstract：:Rotation of the bacterial flagellar motor is powered by a transmembrane gradient of protons or, in some species, sodium ions. The molecular mechanism of coupling between ion flow and motor rotation is not understood. The proteins most closely involved in motor rotation are MotA, MotB, and FliG. MotA and MotB are trans...

journal_title：Journal of bacteriology

authors： Zhou J,Sharp LL,Tang HL,Lloyd SA,Billings S,Braun TF,Blair DF

更新日期：1998-05-01 00:00:00

abstract：:The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...

journal_title：Journal of bacteriology

authors： Ma K,Weiss R,Adams MW

更新日期：2000-04-01 00:00:00

abstract：:In the long-chain n-alkane degrader Acinetobacter sp. strain M-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed con...

journal_title：Journal of bacteriology

authors： Tani A,Ishige T,Sakai Y,Kato N

更新日期：2001-03-01 00:00:00

abstract：:Proteins induced by acid or base, during long-term aerobic or anaerobic growth in complex medium, were identified in Escherichia coli. Two-dimensional gel electrophoresis revealed pH-dependent induction of 18 proteins, nine of which were identified by N-terminal sequencing. At pH 9, tryptophan deaminase (TnaA) was ind...

journal_title：Journal of bacteriology

authors： Blankenhorn D,Phillips J,Slonczewski JL

更新日期：1999-04-01 00:00:00

abstract：:Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...

journal_title：Journal of bacteriology

authors： THORNE CB

更新日期：1962-01-01 00:00:00

abstract：:Overproduction of tryptophanyl-tRNA synthetase increased trp operon expression by reducing transcription termination at the trp attenuator. The total cellular level of charged tRNATrp was not affected by increased levels of the synthetase. We propose that excess synthetase binds charged tRNATrp and reduces the concent...

journal_title：Journal of bacteriology

authors： Das A,Yanofsky C

更新日期：1984-11-01 00:00:00

abstract：:Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...

journal_title：Journal of bacteriology

authors： Dörner E,Boll M

更新日期：2002-07-01 00:00:00

abstract：:Pseudomonas aeruginosa XMG, isolated from soil, utilizes lactate. Here we present a 6.45-Mb assembly of its genome sequence. Besides the lactate utilization mechanism of the strain, the genome sequence may also provide other useful information related to P. aeruginosa, such as identifying genes involved in virulence, ...

journal_title：Journal of bacteriology

authors： Gao C,Hu C,Ma C,Su F,Yu H,Jiang T,Dou P,Wang Y,Qin T,Lv M,Xu P

更新日期：2012-09-01 00:00:00

abstract：:Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 4...

journal_title：Journal of bacteriology

authors： Lugtenberg EJ,v Schijndel-van Dam A

更新日期：1972-04-01 00:00:00

abstract：:A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion c...

journal_title：Journal of bacteriology

authors： Anzai H,Murakami T,Imai S,Satoh A,Nagaoka K,Thompson CJ

更新日期：1987-08-01 00:00:00

abstract：:The gene encoding an extracellular isomalto-dextranase, designated imd, was isolated from the chromosomal DNA of Arthrobacter globiformis T6 and cloned and expressed in Escherichia coli. A single open reading frame consisting of 1,926 base pairs that encoded a polypeptide composed of a signal peptide of 39 amino acids...

journal_title：Journal of bacteriology

authors： Iwai A,Ito H,Mizuno T,Mori H,Matsui H,Honma M,Okada G,Chiba S

更新日期：1994-12-01 00:00:00

abstract：:Pulse-labeled ribonucleic acid (RNA) was extracted from polysomes of sporulating cells of Saccharomyces cerevisiae and characterized in sucrose gradients and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Transfer RNA, ribosomal RNA, and heterodisperse RNA, presumed to be messenger RNA, were synthesized...

journal_title：Journal of bacteriology

authors： Curiale MS,Petryna MM,Mills D

更新日期：1976-05-01 00:00:00

abstract：:The trehalose content in Saccharomyces cerevisiae can be significantly manipulated by including trehalose at an appropriate level in the growth medium. Its uptake is largely dependent on the expression of AGT1, which encodes an alpha-glucoside transporter. The trehalose found in a tps1 mutant of trehalose synthase may...

journal_title：Journal of bacteriology

authors： Plourde-Owobi L,Durner S,Parrou JL,Wieczorke R,Goma G,François J

更新日期：1999-06-01 00:00:00

abstract：:The thermosensitive growth phenotype exerted by runaway-mutant plasmids was suppressed by sublethal doses of the DNA gyrase inhibitors novobiocin or nalidixic acid, although the latter drug was less efficient. A novobiocin-resistant gyrB mutant Escherichia coli strain prevented expression of the runaway phenotype at 3...

journal_title：Journal of bacteriology

authors： Uhlin BE,Nordström K

更新日期：1985-05-01 00:00:00

abstract：:Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...

journal_title：Journal of bacteriology

authors： Shao CP,Lo HR,Lin JH,Hor LI

更新日期：2011-05-01 00:00:00

abstract：:Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...

journal_title：Journal of bacteriology

authors： LUNDGREN DG,COONEY JJ

更新日期：1962-06-01 00:00:00

abstract：:While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...

journal_title：Journal of bacteriology

authors： Al Laham N,Rohde H,Sander G,Fischer A,Hussain M,Heilmann C,Mack D,Proctor R,Peters G,Becker K,von Eiff C

更新日期：2007-06-01 00:00:00

abstract：:The transport system for glycylglycine in Escherichia coli behaves like a shock-sensitive transport system. The initial rate of transport is reduced 85% by subjecting whole cells to osmotic shock, and glycylglycine is not transported by membrane vesicles. The energetics of transport was studied with strain ML 308-225 ...

journal_title：Journal of bacteriology

authors： Cowell JL

更新日期：1974-10-01 00:00:00

abstract：:The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...

journal_title：Journal of bacteriology

authors： Schäferkordt S,Chakraborty T

更新日期：1997-04-01 00:00:00

abstract：:cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of ...

journal_title：Journal of bacteriology

authors： Ambulos NP Jr,Duvall EJ,Lovett PS

更新日期：1987-03-01 00:00:00