Transcript analysis of multiple copies of amo (encoding ammonia monooxygenase) and hao (encoding hydroxylamine oxidoreductase) in Nitrosomonas europaea.

Abstract:

:The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Primer extension reactions were done to identify transcription start sites for these genes, as well as for amoA. Putative sigma(70) promoter sequences were found associated with all but one of the mapped transcription start sites. Primer extensions were done with amoC primers using RNA harvested from cells incubated with and without ammonium. The experiments suggested that N. europaea cells may be able to use different promoters in the presence and absence of ammonium.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hommes NG,Sayavedra-Soto LA,Arp DJ

doi

10.1128/JB.183.3.1096-1100.2001

keywords:

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

1096-100

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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