Function of protonatable residues in the flagellar motor of Escherichia coli: a critical role for Asp 32 of MotB.

abstract：:Members of the pPT23A plasmid family of Pseudomonas syringae play an important role in the interaction of this bacterial pathogen with host plants. Complete sequence analysis of several pPT23A family plasmids (PFPs) has provided a glimpse of the gene content and virulence function of these plasmids. We constructed a m...

journal_title：Journal of bacteriology

authors： Zhao Y,Ma Z,Sundin GW

更新日期：2005-03-01 00:00:00

abstract：:A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...

journal_title：Journal of bacteriology

authors： Oosawa K,Mutoh N,Simon MI

更新日期：1988-06-01 00:00:00

abstract：:Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...

journal_title：Journal of bacteriology

authors： Bonato MC,da Costa Maia JC,Juliani MH

更新日期：1983-06-01 00:00:00

abstract：:Two acid-inducible genes, aniC and aciK, that require anaerobiosis and tyrosine for expression were identified as orf326a encoding a potential amino acid/polyamine antiporter and hyaB encoding hydrogenase I, respectively. Cyclic AMP (cAMP) receptor protein, cAMP, and TyrR, regulator of aromatic amino acid metabolism, ...

journal_title：Journal of bacteriology

authors： Park KR,Giard JC,Eom JH,Bearson S,Foster JW

更新日期：1999-01-01 00:00:00

abstract：:We detected inducible melibiose transport activity in cells of Enterobacter cloacae IID977. H+, but not Na+, was found to be the coupling cation for this transporter. We cloned and sequenced the gene encoding the melibiose transporter. A homology search of a protein sequence database revealed that this melibiose trans...

journal_title：Journal of bacteriology

authors： Okazaki N,Jue XX,Miyake H,Kuroda M,Shimamoto T,Tsuchiya T

更新日期：1997-07-01 00:00:00

abstract：:Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...

journal_title：Journal of bacteriology

authors： Mokrousov I,Vyazovaya A,Narvskaya O

更新日期：2014-05-01 00:00:00

abstract：:Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic sulfur compounds. Here we present the draft genome sequence of Acidithiobacillus caldus ATCC 51756 (the type strain of the spe...

journal_title：Journal of bacteriology

authors： Valdes J,Quatrini R,Hallberg K,Dopson M,Valenzuela PD,Holmes DS

更新日期：2009-09-01 00:00:00

abstract：:In vivo assembly of the Klebsiella aerogenes urease nickel metallocenter requires the presence of UreD, UreF, and UreG accessory proteins and is further facilitated by UreE. Prior studies had shown that urease apoprotein exists in an uncomplexed form as well as in a series of UreD-urease (I.-S. Park, M.B. Carr, and R....

journal_title：Journal of bacteriology

authors： Moncrief MB,Hausinger RP

更新日期：1996-09-01 00:00:00

abstract：:The first three enzymes of the pentachlorophenol (PCP) degradation pathway in Sphingobium chlorophenolicum (formerly Sphingomonas chlorophenolica) ATCC 39723 have been characterized, and the corresponding genes, pcpA, pcpB, and pcpC, have been individually cloned and sequenced. To search for new genes involved in PCP ...

journal_title：Journal of bacteriology

authors： Cai M,Xun L

更新日期：2002-09-01 00:00:00

abstract：:Insertion-duplication mutagenesis was used to generate mutants of Streptococcus pneumoniae that produced truncated forms of PspA (pneumococcal surface protein A). The truncated products, representing from 20 to 80% of the complete PspA molecule, were all secreted from the cell and could be detected in unconcentrated c...

journal_title：Journal of bacteriology

authors： Yother J,Handsome GL,Briles DE

更新日期：1992-01-01 00:00:00

abstract：:The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...

journal_title：Journal of bacteriology

authors： Jones GH

更新日期：1994-03-01 00:00:00

abstract：:An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...

journal_title：Journal of bacteriology

authors： Braun M,Stuber K,Schlatter Y,Wahli T,Kuhnert P,Frey J

更新日期：2002-04-01 00:00:00

abstract：:A nicotinamide adenine dinucleotide-specific L-(+)-lactate dehydrogenase (LDH) (EC 1.11.27) from Actinomyces viscosus T-6-1600 was purified approximately 110-fold by a combination of diethylaminoethyl-cellulose and 0.5 M Agarose A column chromatography. The ldh was stable at 26 C, but was quite labile at temperatures ...

journal_title：Journal of bacteriology

authors： Brown AT,Christian CP,Eifert RL

更新日期：1975-06-01 00:00:00

abstract：:Mutations in the tol-pal genes induce pleiotropic effects such as release of periplasmic proteins into the extracellular medium and hypersensitivity to drugs and detergents. Other outer membrane defective strains such as tolC, lpp, and rfa mutations are also altered in their outer membrane permeability. In this study,...

journal_title：Journal of bacteriology

authors： Bernadac A,Gavioli M,Lazzaroni JC,Raina S,Lloubès R

更新日期：1998-09-01 00:00:00

abstract：:Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

journal_title：Journal of bacteriology

authors： Kocourek J,Ballou CE

更新日期：1969-12-01 00:00:00

abstract：:DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

journal_title：Journal of bacteriology

authors： Washburn BK,Kushner SR

更新日期：1991-04-01 00:00:00

abstract：:The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...

journal_title：Journal of bacteriology

authors： Kennedy JR,Richardson SH

更新日期：1969-12-01 00:00:00

abstract：:Gel retardation experiments indicated the presence in Pseudomonas aeruginosa cell extracts of an arginine-inducible DNA-binding protein that interacts with the control regions for the car and argF operons, encoding carbamoylphosphate synthetase and anabolic ornithine carbamoyltransferase, respectively. Both enzymes ar...

journal_title：Journal of bacteriology

authors： Park SM,Lu CD,Abdelal AT

更新日期：1997-09-01 00:00:00

abstract：:Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J. Bacteriol. 91:609-616. 1966.-Lipid composition of Mycoplasma laidlawii membranes could be significantly changed by var...

journal_title：Journal of bacteriology

authors： Razin S,Tourtellotte ME,McElhaney RN,Pollack JD

更新日期：1966-02-01 00:00:00

abstract：:T4 gene 2 mutants have a pleiotropic phenotype: degradation of injected phage DNA by exonuclease V (ExoV) in the recBCD(+) host cell cytoplasm and a low burst size due, at least in part, to a decreased ability for head-to-tail (H-T) joining. The more N terminal the mutation, the more pronounced is the H-T joining defe...

journal_title：Journal of bacteriology

authors： Wang GR,Vianelli A,Goldberg EB

更新日期：2000-02-01 00:00:00

abstract：:An in vitro system of cell envelopes from Salmonella typhimurium with functional flagella was used to determine the minimal requirements for flagellar rotation. Rotation in the absence of cytoplasmic constituents could be driven either by respiration or by an artificially imposed chemical gradient of protons. No speci...

journal_title：Journal of bacteriology

authors： Ravid S,Eisenbach M

更新日期：1984-06-01 00:00:00

abstract：:Antiserum to Aeromonas hydrophila A6 cell envelopes was shown in a previous study (C. Y. F. Wong, G. Mayrhofer, M. W. Heuzenroeder, H. M. Atkinson, D. M. Quinn, and R. L. P. Flower, FEMS Immunol. Med. Microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. In this study, colony blot ana...

journal_title：Journal of bacteriology

authors： Wong CY,Heuzenroeder MW,Quinn DM,Flower RL

更新日期：1997-06-01 00:00:00

abstract：:Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

journal_title：Journal of bacteriology

authors： Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

更新日期：2012-12-01 00:00:00

abstract：:Escherichia coli strain TP985, which has an insertional mutation in the gene for the periplasmic murein tripeptide binding protein MppA, was previously reported to overproduce MarA and exhibit a multiple-antibiotic resistance (Mar) phenotype (H. Li and J. T. Park, J. Bacteriol. 181:4842-4847, 1999). We found that TP98...

journal_title：Journal of bacteriology

authors： Bina X,Perreten V,Levy SB

更新日期：2003-02-01 00:00:00

abstract：:The chromosomally mediated penicillinase present in three strains of Escherichia coli K-12 has been purified and characterized. Two of the strains carried the ampA gene and the third the wild-type allele. The purification involves release of the enzyme by spheroplast formation, dialysis, chromatography on sulfoethyl c...

journal_title：Journal of bacteriology

authors： Linström EB,Boman HG,Steele BB

更新日期：1970-01-01 00:00:00

abstract：:In Ralstonia eutropha H16, the nitric oxide (NO)-responsive transcriptional activator NorR controls the expression of a dicistronic operon that encodes a membrane-bound NO reductase, NorB, and a protein of unknown function, NorA. The N-terminal domain (NTD) of NorR is responsible for perception of the signal molecule,...

journal_title：Journal of bacteriology

authors： Klink A,Elsner B,Strube K,Cramm R

更新日期：2007-04-01 00:00:00

abstract：:During Bacillus subtilis sporulation, the transient engulfment defect of spoIIB strains is enhanced by spoVG null mutations and suppressed by spoVS null mutations. These mutations have opposite effects on expression of the motility regulon, as the spoVG mutation reduces and the spoVS mutation increases sigmaD-directed...

journal_title：Journal of bacteriology

authors： Perez AR,Abanes-De Mello A,Pogliano K

更新日期：2006-02-01 00:00:00

abstract：:The O-repeating unit of the Escherichia coli O7-specific lipopolysaccharide is made of galactose, mannose, rhamnose, 4-acetamido-4,6-dideoxyglucose, and N-acetyglucosamine. We have recently characterized the genes involved in the biosynthesis of the sugar precursor GDP-mannose occurring in the E. coli O7:K1 strain VW1...

journal_title：Journal of bacteriology

authors： Marolda CL,Valvano MA

更新日期：1995-10-01 00:00:00

abstract：:The aerobic respiratory chain of Escherichia coli can function with either of two different membrane-bound NADH dehydrogenases (NDH-1 and NDH-2) and with either of two ubiquinol oxidases (bd-type and bo-type). The amounts of each of these enzymes present in the E. coli membrane depend on growth conditions in general a...

journal_title：Journal of bacteriology

authors： Calhoun MW,Oden KL,Gennis RB,de Mattos MJ,Neijssel OM

更新日期：1993-05-01 00:00:00

abstract：:Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...

journal_title：Journal of bacteriology

authors： Benning C,Somerville CR

更新日期：1992-10-01 00:00:00