Suppression of engulfment defects in bacillus subtilis by elevated expression of the motility regulon.

abstract：:In an effort to identify the Agrobacterium tumefaciens phosphate regulatory gene(s), we isolated a clone from an A. tumefaciens cosmid library that restored regulated alkaline phosphatase activity to an Escherichia coli phoB mutant. The gene that complemented phoB was localized by subcloning and deletion analysis, and...

journal_title：Journal of bacteriology

authors： Mantis NJ,Winans SC

更新日期：1993-10-01 00:00:00

abstract：:The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

journal_title：Journal of bacteriology

authors： Yokota K,Kanamoto R,Kito M

更新日期：1980-03-01 00:00:00

abstract：:The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...

journal_title：Journal of bacteriology

authors： Rosta S,Mett H

更新日期：1989-01-01 00:00:00

abstract：:Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...

journal_title：Journal of bacteriology

authors： Yoshimura F,Nikaido H

更新日期：1982-11-01 00:00:00

abstract：:Clostridium difficile is the etiological agent of antibiotic-associated diarrhea. Among the factors that may play a role in infection are S-layer proteins (SLPs). Previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpA gene. The high-molecular-w...

journal_title：Journal of bacteriology

authors： Calabi E,Fairweather N

更新日期：2002-07-01 00:00:00

abstract：:As with other inducible enzymes, the induced synthesis of l-arabinose isomerase (l-arabinose ketol isomerase, EC 5.3.1.4) in Salmonella typhimurium is subject to catabolite repression. Of the three catabolite repressors tested, glucose produces maximum repression. Analogues of catabolite repressors like 2-deoxy-d-gluc...

journal_title：Journal of bacteriology

authors： Bhattacharya AK,Chakravorty M

更新日期：1971-04-01 00:00:00

abstract：:Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

journal_title：Journal of bacteriology

authors： Rocco JM,Churchward G

更新日期：2006-03-01 00:00:00

abstract：:A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and s...

journal_title：Journal of bacteriology

authors： Rasmussen KV,Atlung T,Kerszman G,Hansen GE,Hansen FG

更新日期：1983-04-01 00:00:00

abstract：:The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...

journal_title：Journal of bacteriology

authors： Ehlert K,Tschierske M,Mori C,Schröder W,Berger-Bächi B

更新日期：2000-05-01 00:00:00

abstract：:RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...

journal_title：Journal of bacteriology

authors： Zhang S,Reeves A,Woodbury RL,Haldenwang WG

更新日期：2005-12-01 00:00:00

abstract：:Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...

journal_title：Journal of bacteriology

authors： Chopra I,Shales SW

更新日期：1980-10-01 00:00:00

abstract：:A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it. ...

journal_title：Journal of bacteriology

authors： Tingle MA,Neidhardt FC

更新日期：1969-05-01 00:00:00

abstract：:The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF- mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the respon...

journal_title：Journal of bacteriology

authors： Zeilstra-Ryalls JH,Schornberg KL

更新日期：2006-01-01 00:00:00

abstract：:Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-bindin...

journal_title：Journal of bacteriology

authors： Abraham SN,Land M,Ponniah S,Endres R,Hasty DL,Babu JP

更新日期：1992-08-01 00:00:00

abstract：:Mutants deficient in the production of bacteriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus. Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were ...

journal_title：Journal of bacteriology

authors： Pierson BK,Keith LM,Leovy JG

更新日期：1984-07-01 00:00:00

abstract：:We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the tr...

journal_title：Journal of bacteriology

authors： Bartlett MS,Gourse RL

更新日期：1994-09-01 00:00:00

abstract：:The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...

journal_title：Journal of bacteriology

authors： David S,van der Rest ME,Driessen AJ,Simons G,de Vos WM

更新日期：1990-10-01 00:00:00

abstract：:The following results with an in vitro replication system utilizing a plasmid RK2 DNA-membrane complex indicate that the essential trfA-encoded replication protein of RK2 is present and active in the complex. (i) A complex extracted from a conditional replication mutant of RK2, which contains a temperature-sensitive m...

journal_title：Journal of bacteriology

authors： Kornacki JA,Firshein W

更新日期：1986-07-01 00:00:00

abstract：:Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...

journal_title：Journal of bacteriology

authors： Kwon M,Lee J,Lee C,Park C

更新日期：2012-04-01 00:00:00

abstract：:The enhancer-binding protein NIFA is required for transcriptional activation of nif promoters by the alternative holoenzyme form of RNA polymerase, which contains the sigma factor sigma 54 (sigma N). NIFA hydrolyzes nucleoside triphosphates to catalyze the isomerization of closed promoter complexes to transcriptionall...

journal_title：Journal of bacteriology

authors： Eydmann T,Söderbäck E,Jones T,Hill S,Austin S,Dixon R

更新日期：1995-03-01 00:00:00

abstract：:The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dis...

journal_title：Journal of bacteriology

authors： Inamine GS,Dubnau D

更新日期：1995-06-01 00:00:00

abstract：UNLABELLED:Escherichia coli RtcB is a founding member of a family of manganese-dependent RNA repair enzymes that join RNA 2′,3′-cyclic phosphate (RNA>p) or RNA 3′-phosphate (RNAp) ends to 5′-OH RNA (HORNA) ends in a multistep pathway whereby RtcB (i) hydrolyzes RNA>p to RNAp, (ii) transfers GMP from GTP to RNAp to form...

journal_title：Journal of bacteriology

authors： Maughan WP,Shuman S

更新日期：2016-03-31 00:00:00

abstract：:Streptococcus bovis possesses two sugar phosphate phosphatases (Pases). Pase I is a soluble enzyme that is inhibited by the membrane fractions from lactose-grown cells and is insensitive to activation by S46D HPr, an analog of HPr(ser-P) of the sugar phosphotransferase system. Pase II is a membrane-associated enzyme t...

journal_title：Journal of bacteriology

authors： Cook GM,Ye JJ,Russell JB,Saier MH Jr

更新日期：1995-12-01 00:00:00

abstract：:Scanning electron microscopy has been employed to examine events in the release and development of mitospores of the aquatic fungus, Allomyces arbuscula. Among the salient features of spore release from the mitosporangium is the digestion of the inner matrix of the exit papillum. Hydrolysis appears to begin at the out...

journal_title：Journal of bacteriology

authors： Seale TW,Delehanty J,Runyan RB

更新日期：1974-12-01 00:00:00

abstract：:Nitrogenase activities were determined from maximum acetylene reduction rates for mutant strains of Azotobacter vinelandii which are unable to fix N2 in the presence of molybdenum (Nif-) but undergo phenotypic reversal to Nif+ under conditions of Mo deficiency. The system responsible for N2 fixation under these condit...

journal_title：Journal of bacteriology

authors： Bishop PE,Jarlenski DM,Hetherington DR

更新日期：1982-06-01 00:00:00

abstract：:Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...

journal_title：Journal of bacteriology

authors： Alphen WV,Lugtenberg B

更新日期：1977-08-01 00:00:00

abstract：:Accumulation of intracellular allantoin and allantoate is mediated by two distinct active transport systems in Saccharomyces cerevisiae. Allantoin transport (DAL4 gene) is inducible, while allantoate uptake is constitutive (it occurs at full levels in the absence of any allantoate-related compounds from the culture me...

journal_title：Journal of bacteriology

authors： Chisholm VT,Lea HZ,Rai R,Cooper TG

更新日期：1987-04-01 00:00:00

abstract：:An extracellular, 700,000-Mr multiprotein complex that catalyzed the hydrolysis of crystalline cellulose (Avicel) was isolated from cultures of Clostridium sp. strain C7, a mesophile from freshwater sediment. In addition to cellulose (Avicel, ball-milled filter paper), the multiprotein complex hydrolyzed carboxymethyl...

journal_title：Journal of bacteriology

authors： Cavedon K,Leschine SB,Canale-Parola E

更新日期：1990-08-01 00:00:00

abstract：:A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cros...

journal_title：Journal of bacteriology

authors： Chandler PM,Krishnapillai V

更新日期：1977-05-01 00:00:00

abstract：:Multicopy single-stranded DNA is found as a small single-stranded RNA-DNA complex in certain wild-type strains of Escherichia coli as well as in other gram-negative bacteria. Using the promoter region of the previously characterized retron-Ec107 from E. coli ECOR70, I constructed a chromosomally located lacZ operon fu...

journal_title：Journal of bacteriology

authors： Herzer PJ

更新日期：1996-08-01 00:00:00