Abstract:
:RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate upstream and downstream neighbors. In the current work we demonstrate that RsbT's ability to function is strongly influenced by coexpression with these adjoining genes. When rsbT is expressed at a site displaced from rsbS and rsbU, RsbT accumulates but it is unable to activate sigma(B) following stress. RsbT activity is restored if rsbT is cotranscribed at the alternative site with the genes that normally abut it. Additionally, an rsbS allele whose product allows constitutively high RsbT-dependent sigma(B) activity displays this activity in rsbS merodiploid strains only when cotranscribed with rsbT and is recessive to a wild-type rsbS allele only if the wild-type rsbS gene is not cotranscribed with an rsbT gene of its own. The data suggest that RsbS and RsbT are synthesized in equivalent amounts and interact coincidently with their synthesis to form stable regulatory complexes that maintain RsbT in a state from which it can be stress activated.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zhang S,Reeves A,Woodbury RL,Haldenwang WGdoi
10.1128/JB.187.24.8520-8525.2005keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
8520-5issue
24eissn
0021-9193issn
1098-5530pii
187/24/8520journal_volume
187pub_type
杂志文章abstract::We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1192-1195.1989
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doi:10.1128/jb.178.11.3350-3352.1996
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journal_title:Journal of bacteriology
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doi:10.1128/JB.94.5.1431-1436.1967
更新日期:1967-11-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.143.3.1498-1500.1980
更新日期:1980-09-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.255-263.1982
更新日期:1982-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1602-1608.1989
更新日期:1989-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2781-2787.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.100-106.1974
更新日期:1974-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.905-910.1975
更新日期:1975-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.1109-1114.1984
更新日期:1984-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.874-875.1973
更新日期:1973-05-01 00:00:00
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更新日期:1999-02-01 00:00:00
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更新日期:1972-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.553-562.1994
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.163.3.890-894.1985
更新日期:1985-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.99-103.1989
更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1062-1070.1983
更新日期:1983-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-13
更新日期:2013-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.11.3325-3332.2003
更新日期:2003-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.186-195.1983
更新日期:1983-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.569-577.1978
更新日期:1978-05-01 00:00:00