Enzymes involved in the assimilation of one-carbon units by Pseudomonas MS.

Abstract:

:Pseudomonas MS can grow on methylamine and a number of other compounds containing C1 units as a sole source of carbon and energy. Assimilation of carbon into cell material occurs via the "serine pathway" since enzymes of this pathway are induced after growth on methylamine, but not malate or acetate. A mutant has been isolated which is unable to grow on methylamine or any other related substrate providing C1 units. This mutant is also unable to grow on acetate. Measurment of enzyme activities in cell-free extracts of wild-type cells showed that growth on methylamine caused induction of isocitrate lyase, a key enzyme in the glyoxylate cycle. The mutant organism lacks malate lyase, a key enzyme of the serine pathway, and isocitrate lyase as well. These results suggest that utilization of C1 units by Pseudomonas MS results in the net accumulation of acetate which is then assimilated into cell material via the glyoxylate cycle.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wagner C,Levitch ME

doi

10.1128/JB.122.3.905-910.1975

subject

Has Abstract

pub_date

1975-06-01 00:00:00

pages

905-10

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

122

pub_type

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