Imprecise excision of plasmid pE194 from the chromosomes of Bacillus subtilis pE194 insertion strains.

Abstract:

:Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, Mol. Gen. Genet. 189:58-68, 1983). We have investigated nine excision plasmids, carrying insert DNA 1 to 6 kbp in length, either in a complete pE194 or in a partially deleted pE194 copy. Type 1 (additive) excision plasmids have the left- and right-junction DNAs preserved as 13-bp direct repeats (5'-GGGGAGAAAACAT-3') corresponding to the region between positions 864 and 876 in pE194. In type 2 (substitutive) excision plasmids, a conserved 13-bp sequence remains only at the right junction while the left junction has been deleted during the excision process. The type 3 excision plasmid carries at each junction the tetranucleotide 5'-TCCC-3', present in pE194 between positions 1995 and 1998. Although we isolated the excision plasmids from different integration mutants, the insert DNAs of eight independently isolated plasmids showed striking sequence homology, suggesting that they originated from one distinct region of the B. subtilis chromosome. Thus, we postulate that imprecise excision of pE194 occurs most frequently after its translocation from the original insertion site into a preferred excision site within the host chromosome. The imprecise excision from this site occurs at excision breakpoints outside the pE194-chromosome junctions in a chromosomal region which remains to be investigated further.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Conrad B,Bashkirov VI,Hofemeister J

doi

10.1128/jb.174.21.6997-7002.1992

keywords:

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

6997-7002

issue

21

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

杂志文章
  • Acetate utilization is inhibited by benzoate in Alcaligenes eutrophus: evidence for transcriptional control of the expression of acoE coding for acetyl coenzyme A synthetase.

    abstract::During batch growth of Alcaligenes eutrophus on benzoate-acetate mixtures, benzoate was the preferred substrate, with acetate consumption being delayed until the rate of benzoate consumption had diminished. This effect was attributed to a transcriptional control of the synthesis of acetyl coenzyme A (acetyl-CoA) synth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.20.5826-5833.1995

    authors: Ampe F,Lindley ND

    更新日期:1995-10-01 00:00:00

  • Phospholipids and lipopolysaccharide of Aeromonas hydrophila.

    abstract::The phospholipids and lipopolysaccharide of Aeromonas hydrophila were characterized. Phosphatidylethanolamine and phosphatidylglycerol were the major phospholipid components. The outer membrane contained more phosphatidylethanolamine and less phosphatidylglycerol than the inner membrane, and the phospholipids of the o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.463-465.1985

    authors: Howard SP,Buckley JT

    更新日期:1985-01-01 00:00:00

  • An N-terminal mutation in the bacteriophage T4 motA gene yields a protein that binds DNA but is defective for activation of transcription.

    abstract::The bacteriophage T4 MotA protein is a transcriptional activator of T4-modified host RNA polymerase and is required for activation of the middle class of T4 promoters. MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box consensus sequence [(t/a)(t/a)TGCTT(t/c)A]. We report th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.21.6133-6139.1996

    authors: Gerber JS,Hinton DM

    更新日期:1996-11-01 00:00:00

  • Clustering of mutations affecting central pathway enzymes of carbohydrate catabolism in Pseudomonas aeruginosa.

    abstract::Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated trans...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.3.1123-1129.1983

    authors: Roehl RA,Feary TW,Phibbs PV Jr

    更新日期:1983-12-01 00:00:00

  • Oxygen-insensitive nitroreductases: analysis of the roles of nfsA and nfsB in development of resistance to 5-nitrofuran derivatives in Escherichia coli.

    abstract::Nitroheterocyclic and nitroaromatic compounds constitute an enormous range of chemicals whose potent biological activity has significant human health and environmental implications. The biological activity of nitro-substituted compounds is derived from reductive metabolism of the nitro moiety, a process catalyzed by a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.21.5529-5539.1998

    authors: Whiteway J,Koziarz P,Veall J,Sandhu N,Kumar P,Hoecher B,Lambert IB

    更新日期:1998-11-01 00:00:00

  • Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.

    abstract::Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.338-340.1986

    authors: Koyama Y,Hoshino T,Tomizuka N,Furukawa K

    更新日期:1986-04-01 00:00:00

  • 3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product.

    abstract::The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.11.3632-3638.1997

    authors: Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

    更新日期:1997-06-01 00:00:00

  • Homology of mycoplasma plasmid pADB201 and staphylococcal plasmid pE194.

    abstract::The complete nucleotide sequence of pADB201, a 1.7-kilobase cryptic plasmid from Mycoplasma mycoides subsp. mycoides, is reported. The sequence contains a single large open reading frame capable of coding for a polypeptide of up to 198 codons long. The sequence of the putative polypeptide shows significant similarity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.593-595.1989

    authors: Bergemann AD,Whitley JC,Finch LR

    更新日期:1989-01-01 00:00:00

  • Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase.

    abstract::A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identifi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5968-5979.1990

    authors: Crouzet J,Cauchois L,Blanche F,Debussche L,Thibaut D,Rouyez MC,Rigault S,Mayaux JF,Cameron B

    更新日期:1990-10-01 00:00:00

  • Pyrithiamine adaptation of Staphylococcus aureus. I. Adaptation and carbohydrate utilization.

    abstract::Das, S. K. (University of Calcutta, Calcutta, India) and G. C. Chatterjee. Pyrithiamine adaptation of Staphylococcus aureus. I. Adaptation and carbohydrate utilization. J. Bacteriol. 83:1251-1259. 1962.-Staphylococcus aureus has been adapted to pyrithiamine, a thiamine analogue; as a result of this adaptation, the col...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1251-1259.1962

    authors: DAS SK,CHATTERJEE GC

    更新日期:1962-06-01 00:00:00

  • Antibacterial nitroacridine, Nitroakridin 3582: binding to nucleic acids in vitro and effects on selected cell-free model systems of macromolecular biosynthesis.

    abstract::Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1026-1033.1971

    authors: Wolfe AD,Cook TM,Hahn FE

    更新日期:1971-12-01 00:00:00

  • Characterization of the exopolygalacturonate lyase PelX of Erwinia chrysanthemi 3937.

    abstract::Erwinia chrysanthemi 3937 secretes several pectinolytic enzymes, among which eight isoenzymes of pectate lyases with an endo-cleaving mode (PelA, PelB, PelC, PelD, PelE, PelI, PelL, and PelZ) have been identified. Two exo-cleaving enzymes, the exopolygalacturonate lyase, PelX, and an exo-poly-alpha-D-galacturonosidase...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.5.1652-1663.1999

    authors: Shevchik VE,Kester HC,Benen JA,Visser J,Robert-Baudouy J,Hugouvieux-Cotte-Pattat N

    更新日期:1999-03-01 00:00:00

  • NarK enhances nitrate uptake and nitrite excretion in Escherichia coli.

    abstract::narK mutants of Escherichia coli produce wild-type levels of nitrate reductase but, unlike the wild-type strain, do not accumulate nitrite when grown anaerobically on a glucose-nitrate medium. Comparison of the rates of nitrate and nitrite metabolism in cultures growing anaerobically on glucose-nitrate medium revealed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.11.3303-3310.1991

    authors: DeMoss JA,Hsu PY

    更新日期:1991-06-01 00:00:00

  • Overexpression of vsr in Escherichia coli is mutagenic.

    abstract::Overexpression of vsr in Escherichia coli stimulates transition and frameshift mutations. The pattern of mutations suggests that mutagenesis is due to saturation or inactivation of dam-directed mismatch repair. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.14.4294-4296.1996

    authors: Doiron KM,Viau S,Koutroumanis M,Cupples CG

    更新日期:1996-07-01 00:00:00

  • Mutants suppressing novobiocin hypersensitivity of a mukB null mutation.

    abstract::The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation. All suppressor mutations are localized in or n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.13.3690-3695.2003

    authors: Adachi S,Hiraga S

    更新日期:2003-07-01 00:00:00

  • Physiological and biochemical characterization of the soluble formate dehydrogenase, a molybdoenzyme from Alcaligenes eutrophus.

    abstract::Organoautotrophic growth of Alcaligenes eutrophus on formate was dependent on the presence of molybdate in the medium. Supplementation of the medium with tungstate lead to growth cessation. Corresponding effects of these anions were observed for the activity of the soluble, NAD(+)-linked formate dehydrogenase (S-FDH; ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4719-4728.1993

    authors: Friedebold J,Bowien B

    更新日期:1993-08-01 00:00:00

  • Transglycosylase and endopeptidase participate in the degradation of murein during autolysis of Escherichia coli.

    abstract::The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.3.759-765.1986

    authors: Kitano K,Tuomanen E,Tomasz A

    更新日期:1986-09-01 00:00:00

  • Genome sequence of Yersinia pestis KIM.

    abstract::We present the complete genome sequence of Yersinia pestis KIM, the etiologic agent of bubonic and pneumonic plague. The strain KIM, biovar Mediaevalis, is associated with the second pandemic, including the Black Death. The 4.6-Mb genome encodes 4,198 open reading frames (ORFs). The origin, terminus, and most genes en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.16.4601-4611.2002

    authors: Deng W,Burland V,Plunkett G 3rd,Boutin A,Mayhew GF,Liss P,Perna NT,Rose DJ,Mau B,Zhou S,Schwartz DC,Fetherston JD,Lindler LE,Brubaker RR,Plano GV,Straley SC,McDonough KA,Nilles ML,Matson JS,Blattner FR,Perry RD

    更新日期:2002-08-01 00:00:00

  • Roles of the Site 2 Protease Eep in Staphylococcus aureus.

    abstract::In Enterococcus faecalis, the site 2 protease Eep generates sex pheromones, including cAM373. Intriguingly, in Staphylococcus aureus, a peptide similar to cAM373, named cAM373_SA, is produced from the camS gene. Here, we report that the staphylococcal Eep homolog is not only responsible for the production of cAM373_SA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00046-20

    authors: Cheng D,Lv H,Yao Y,Cheng S,Huang Q,Wang H,Liu X,Bae T,Li M,Liu Q

    更新日期:2020-07-09 00:00:00

  • Bacterial plasmids that carry two functional centromere analogs are stable and are partitioned faithfully.

    abstract::The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.742-745.1984

    authors: Austin SJ

    更新日期:1984-05-01 00:00:00

  • Burkholderia spp. alter Pseudomonas aeruginosa physiology through iron sequestration.

    abstract::Pseudomonas aeruginosa and members of the Burkholderia cepacia complex often coexist in both the soil and the lungs of cystic fibrosis patients. To gain an understanding of how these different species affect each other's physiology when coexisting, we performed a screen to identify P. aeruginosa genes that are induced...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.8.2376-2384.2004

    authors: Weaver VB,Kolter R

    更新日期:2004-04-01 00:00:00

  • Distribution of megaplasmids in Lactobacillus salivarius and other lactobacilli.

    abstract::The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00447-07

    authors: Li Y,Canchaya C,Fang F,Raftis E,Ryan KA,van Pijkeren JP,van Sinderen D,O'Toole PW

    更新日期:2007-09-01 00:00:00

  • ArgR and AhrC are both required for regulation of arginine metabolism in Lactococcus lactis.

    abstract::The DNA binding proteins ArgR and AhrC are essential for regulation of arginine metabolism in Escherichia coli and Bacillus subtilis, respectively. A unique property of these regulators is that they form hexameric protein complexes, mediating repression of arginine biosynthetic pathways as well as activation of argini...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.4.1147-1157.2004

    authors: Larsen R,Buist G,Kuipers OP,Kok J

    更新日期:2004-02-01 00:00:00

  • Characterization of exochelins of Mycobacterium avium: evidence for saturated and unsaturated and for acid and ester forms.

    abstract::Mycobacterium avium secretes iron-binding siderophores called exochelins. The exochelins from M. avium have previously been reported to have unsaturated side chains that terminate in carboxylic acid. In contrast, our data show the side chains to be both saturated and unsaturated and to terminate with either a carboxyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.21.6394-6398.1996

    authors: Wong DK,Gobin J,Horwitz MA,Gibson BW

    更新日期:1996-11-01 00:00:00

  • Isolation and characterization of a second exe operon required for extracellular protein secretion in Aeromonas hydrophila.

    abstract::Strain C5.84 is a Tn5-751 insertion mutant of Aeromonas hydrophila which is unable to secrete extracellular proteins, instead accumulating them in the periplasm (B. Jiang and S.P. Howard, J. Bacteriol. 173:1241-1249, 1991). A 3.5-kb BglII fragment which complements this mutation was isolated from the chromosome of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.22.6819-6826.1994

    authors: Jahagirdar R,Howard SP

    更新日期:1994-11-01 00:00:00

  • Translation rates of individual codons are not correlated with tRNA abundances or with frequencies of utilization in Escherichia coli.

    abstract::We analyzed 12 individual codons, which differed widely with respect to the frequency of use in Escherichia coli and the abundance of the corresponding tRNAs, for their influence on the coupling between transcription and translation. This was probed by determining the effects of codon substitutions in the leader pepti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.5812-5816.1989

    authors: Bonekamp F,Dalbøge H,Christensen T,Jensen KF

    更新日期:1989-11-01 00:00:00

  • Cloning and expression in Escherichia coli of an extremely thermostable oligo-1,6-glucosidase gene from Bacillus thermoglucosidasius.

    abstract::The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.1219-1222.1989

    authors: Watanabe K,Iha H,Ohashi A,Suzuki Y

    更新日期:1989-02-01 00:00:00

  • INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS REPLICATION, CYTOPATHOLOGY, AND PLAQUE FORMATION IN THE PRESENCE AND ABSENCE OF NUCLEIC ACID ANALOGUES.

    abstract::Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.2.446-453.1964

    authors: STEVENS JG,GROMAN NB

    更新日期:1964-02-01 00:00:00

  • DETECTION AND PERSISTENCE OF VI ANTIGEN IN TISSUES OF ACTIVELY IMMUNIZED MICE.

    abstract::Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Detection and persistence of Vi antigen in tissues of actively immunized mice. J. Bacteriol. 89:776-781. 1965.-The presence, distribution, and persistence of Vi antigen in mouse tissue was determined by me...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.776-781.1965

    authors: GAINES S,CURRIE JA,TULLY JG

    更新日期:1965-03-01 00:00:00

  • Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in spirillum serpens and bacillus subtilis.

    abstract::Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.870-875.1966

    authors: Martinez RJ,Gordee EZ

    更新日期:1966-02-01 00:00:00