Abstract:
:Lee, Spencer H. S. (Dalhousie University, Halifax, Nova Scotia). Effect of 6-aminonicotinamide on chick-embryo tissue cultures infected with vaccinia virus. J. Bacteriol. 88:885-892. 1964.-6-Aminonicotinamide (6-AN) was shown to render chick-embryo fibroblast (CEF) tissue cultures refractory to infection with vaccinia virus. Pretreatment of CEF cultures for 8 hr at 36 C with 5 mug/ml of 6-AN prior to addition of vaccinia virus reduced the titer of active virus to about 0.4% of the levels obtained in the untreated controls, when measured by the number of plaque-forming units per milliliter. The addition of 5 mug of 6-AN to CEF cultures before virus infection retarded the progress of vaccinial infection by 8 to 10 hr at 36 C. The inhibitory effect caused by 5 mug/ml of 6-AN could be reversed by the simultaneous addition of 5 to 50 mug/ml of nicotinamide, but not if added at a later time. 6-AN appears to act on the host cell and not on vaccinia virus. For these tests, a simple plaque assay method was devised, by use of a fluid overlay technique with screw-capped tubes (16 by 125 mm) containing CEF cultures.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
LEE SHdoi
10.1128/JB.88.4.885-892.1964keywords:
subject
Has Abstractpub_date
1964-10-01 00:00:00pages
885-92eissn
0021-9193issn
1098-5530journal_volume
88pub_type
杂志文章abstract::Sixty-four mutants were isolated from the LT-2 wild-type strain of Salmonella typhimurium by selecting for chromate resistance. The majority of lesions were shown to lie in the cysA gene. (i) The mutants cannot take up sulfate, a finding which verifies the role of cysA in sulfate transport. In addition, 52 sulfate-tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.1053-1062.1971
更新日期:1971-03-01 00:00:00
abstract::Escherichia coli recF mutants are hypermutable when treated with methyl methanesulfonate (G. C. Walker, Mol. Gen. Genet. 152:93-103, 1977). In this study, methylation hypermutability of recF mutant strains was examined, and it was found that recF+ is required for normal induction of the adaptive response to alkylation...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.99-103.1989
更新日期:1989-01-01 00:00:00
abstract::In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01095-13
更新日期:2014-01-01 00:00:00
abstract::Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.9.3273-3279.2006
更新日期:2006-05-01 00:00:00
abstract:UNLABELLED:The 2-thiouridine (s(2)U) modification of the wobble position in glutamate, glutamine, and lysine tRNA molecules serves to stabilize the anticodon structure, improving ribosomal binding and overall efficiency of the translational process. Biosynthesis of s(2)U in Escherichia coli requires a cysteine desulfur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02625-14
更新日期:2015-06-01 00:00:00
abstract::Dehority, B. A. (Ohio Agricultural Experiment Station, Wooster). Degradation and utilization of isolated hemicellulose by pure cultures of cellulolytic rumen bacteria. J. Bacteriol. 89:1515-1520. 1965.-Hemicelluloses isolated from flax, corn hulls, alfalfa, oat hulls, and fescue grass were used as sole energy sources ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.6.1515-1520.1965
更新日期:1965-06-01 00:00:00
abstract::Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.2.439-446.1970
更新日期:1970-08-01 00:00:00
abstract::Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00468-16
更新日期:2016-09-22 00:00:00
abstract::A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.820-825.1984
更新日期:1984-06-01 00:00:00
abstract::Acetyl coenzyme A synthetase (Acs) activates acetate to acetyl coenzyme A through an acetyladenylate intermediate; two other enzymes, acetate kinase (Ack) and phosphotransacetylase (Pta), activate acetate through an acetyl phosphate intermediate. We subcloned acs, the Escherichia coli open reading frame purported to e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2878-2886.1995
更新日期:1995-05-01 00:00:00
abstract:UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00466-15
更新日期:2015-11-01 00:00:00
abstract::The glmU gene product of Escherichia coli was recently identified as the N-acetylglucosamine-1-phosphate uridyltransferase activity which catalyzes the formation of UDP-N-acetylglucosamine, an essential precursor for cell wall peptidoglycan and lipopolysaccharide biosyntheses (D. Mengin-Lecreulx and J. van Heijenoort,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5788-5795.1994
更新日期:1994-09-01 00:00:00
abstract::The dimorphic fungus Mucor racemosus was found to contain the cyclic nucleotide guanosine 3',5'-monophosphate (cGMP). Approximately equivalent amounts of the compound were found in ungerminated spores, yeastlike cells, and mycelia. Germinating spores contained severalfold higher amounts of cGMP than the other cell for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.1226-1228.1976
更新日期:1976-03-01 00:00:00
abstract::Expression of the major porin structural genes, ompF and ompC, is influenced by medium osmotic strength and requires the products of two regulatory genes, ompR and envZ. To help define the sites required for the expression of both porin genes, we have used a novel selection to identify mutations that decrease ompF tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.3.1054-1060.1985
更新日期:1985-06-01 00:00:00
abstract::An IMP dehydrogenase gene was isolated from Candida albicans on a approximately 2.9-kb XbaI genomic DNA fragment. The putative Candida IMP dehydrogenase gene (IMH3) encodes a protein of 521 amino acids with extensive sequence similarity to the IMP dehydrogenases of Saccharomyces cerevisiae and various other organisms....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2331-2338.1997
更新日期:1997-04-01 00:00:00
abstract::The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00122-06
更新日期:2006-06-01 00:00:00
abstract::Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, have been cloned and sequenced, and they encode principal sigma factors of the RNA polymerase. The deduced amino acid sequences of SigA and SigB showed very high similarities to th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.2.550-553.1996
更新日期:1996-01-01 00:00:00
abstract::Penicillin-binding proteins (PBPs), although characterized from several organisms, have so far not been studied in mycobacteria. The present study is the first characterization of a PBP from Mycobacterium smegmatis. The PBP was purified by solubilization of the membranes with Triton X-100 and successive chromatography...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4829-4832.1992
更新日期:1992-07-01 00:00:00
abstract::It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.3.1205-1210.2006
更新日期:2006-02-01 00:00:00
abstract::Growth of Streptococcus faecalis in the presence of l-malate resulted in the induction of a "malic enzyme" [l-malate:nicotinamide adenine dinucleotide (NAD) oxidoreductase (decarboxylating), E.C. 1.1.1.39]. Synthesis of the malic enzyme did not appear to be subject to catabolite repression by intermediate products of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.705-711.1969
更新日期:1969-05-01 00:00:00
abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1968-09-01 00:00:00
abstract::The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported on a direct cooperation. To investigate the possible interaction of the putative RNA helicases CshA and CshB and the CSP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.240-248.2006
更新日期:2006-01-01 00:00:00
abstract::Thermococcus sp. strain CL1 is a hyperthermophilic, anaerobic, and heterotrophic archaeon isolated from a Paralvinella sp. polychaete worm living on an active deep-sea hydrothermal sulfide chimney on the Cleft Segment of the Juan de Fuca Ridge. To further understand the distinct characteristics of this archaeon at the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01016-12
更新日期:2012-09-01 00:00:00
abstract::We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2195-2201.1989
更新日期:1989-04-01 00:00:00
abstract::Two nrdF genes, nrdF1 and nrdF2, encoding the small subunit (R2) of ribonucleotide reductase (RR) from Mycobacterium tuberculosis have 71% identity at the amino acid level and are both highly homologous with Salmonella typhimurium R2F. The calculated molecular masses of R2-1 and R2-2 are 36,588 (322 amino acids [aa]) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.20.6408-6415.1997
更新日期:1997-10-01 00:00:00
abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.14.4066-4073.2003
更新日期:2003-07-01 00:00:00
abstract::A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.544-549.1969
更新日期:1969-02-01 00:00:00
abstract::Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1296-1303.2000
更新日期:2000-03-01 00:00:00
abstract::Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.6.1460-1464.1965
更新日期:1965-06-01 00:00:00
abstract::The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.22.6728-6731.2003
更新日期:2003-11-01 00:00:00