Characterization of two genes encoding the Mycobacterium tuberculosis ribonucleotide reductase small subunit.

abstract：:Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

journal_title：Journal of bacteriology

authors： Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

更新日期：2005-06-01 00:00:00

abstract：:The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

journal_title：Journal of bacteriology

authors： Reniere ML

更新日期：2018-08-10 00:00:00

abstract：:Previous investigations have demonstrated the existence of five Histoplasma capsulatum serotypes. Available specific fluorescent-antibody reagents stain only four of the five serotypes. Antibodies produced against the most complete H. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagen...

journal_title：Journal of bacteriology

authors： Kaufman L,Blumer S

更新日期：1968-04-01 00:00:00

abstract：:Several enzymes and metabolites concerned with carbohydrate metabolism were examined in mice infected with Listeria monocytogenes. Liver glycogen and glucose decreased parallel to severity of infection. The concentration of glucose in the blood fell to abnormally low levels with a hypoglycemia being most evident at 72...

journal_title：Journal of bacteriology

authors： Wilder MS,Sword CP

更新日期：1967-02-01 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:Interest in finding small RNAs (sRNAs) in bacteria has significantly increased in recent years due to their regulatory functions. Development of high-throughput methods and more sophisticated computational algorithms has allowed rapid identification of sRNA candidates in different species. However, given their various...

journal_title：Journal of bacteriology

authors： Tsai CH,Liao R,Chou B,Palumbo M,Contreras LM

更新日期：2015-01-01 00:00:00

abstract：:An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...

journal_title：Journal of bacteriology

authors： Lackey D,Walker GC,Keng T,Linn S

更新日期：1977-08-01 00:00:00

abstract：:Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

journal_title：Journal of bacteriology

authors： Currier TC,Haury JF,Wolk CP

更新日期：1977-03-01 00:00:00

abstract：:The determinant of resistance to fosfomycin of the Serratia marcescens plasmid pOU900 was transposed into the plasmid ColE1 and into the plasmid RP4 in the absence of the RecA function of the host. In each case, the acquisition of fosfomycin resistance was correlated with the presence of a discrete piece of DNA, unifo...

journal_title：Journal of bacteriology

authors： García-Lobo JM,Ortiz JM

更新日期：1982-07-01 00:00:00

abstract：:Transcriptional factor CarD is the only reported prokaryotic analog of eukaryotic high-mobility-group A (HMGA) proteins, in that it has contiguous acidic and AT hook DNA-binding segments and multifunctional roles in Myxococcus xanthus carotenogenesis and fruiting body formation. HMGA proteins are small, randomly struc...

journal_title：Journal of bacteriology

authors： Cayuela ML,Elías-Arnanz M,Peñalver-Mellado M,Padmanabhan S,Murillo FJ

更新日期：2003-06-01 00:00:00

abstract：:Fluctuations in osmolarity are one of the most prevalent stresses to which bacteria must adapt, both hypo- and hyperosmotic conditions. Most bacteria cope with high osmolarity by accumulating compatible solutes (osmolytes) in the cytoplasm to maintain the turgor pressure of the cell. Vibrio parahaemolyticus, a halophi...

journal_title：Journal of bacteriology

authors： Gregory GJ,Dutta A,Parashar V,Boyd EF

更新日期：2020-11-19 00:00:00

abstract：:The Arc (anoxic redox control) two-component signal transduction system of Escherichia coli, which comprises the tripartite ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous operons in response to redox conditions of growth. We demonstrate that the arcA and arcB genes of Haemophi...

journal_title：Journal of bacteriology

authors： Georgellis D,Kwon O,Lin EC,Wong SM,Akerley BJ

更新日期：2001-12-01 00:00:00

abstract：:Escherichia coli sigma54-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator. Sigma70-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF deltaHTH. Negative autoregulation of PspF...

journal_title：Journal of bacteriology

authors： Jovanovic G,Dworkin J,Model P

更新日期：1997-08-01 00:00:00

abstract：:The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, ...

journal_title：Journal of bacteriology

authors： Charbonneau ME,Girard V,Nikolakakis A,Campos M,Berthiaume F,Dumas F,Lépine F,Mourez M

更新日期：2007-12-01 00:00:00

abstract：:DNA sequence analysis revealed that the putative yhdJ DNA methyltransferase gene of Escherichia coli is 55% identical to the Nostoc sp. strain PCC7120 gene encoding DNA methyltransferase AvaIII, which methylates adenine in the recognition sequence, ATGCAT. The yhdJ gene was cloned, and the enzyme was overexpressed and...

journal_title：Journal of bacteriology

authors： Kossykh VG,Lloyd RS

更新日期：2004-04-01 00:00:00

abstract：:Plasmids encoding the structural genes for the Rhodobacter capsulatus and Rhodobacter sphaeroides cytochrome (cyt) bc1 complexes were introduced into strains of R. capsulatus lacking the cyt bc1 complex, with and without cyt c2. The R. capsulatus merodiploids contained higher than wild-type levels of cyt bc1 complex, ...

journal_title：Journal of bacteriology

authors： Davidson E,Prince RC,Haith CE,Daldal F

更新日期：1989-11-01 00:00:00

abstract：:The Firmicutes Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes convert thiosulfate, forming sulfur globules inside and outside cells. X-ray absorption near-edge structure analysis revealed that the sulfur consisted mainly of sulfur chains with organic end groups similar to sulfur formed ...

journal_title：Journal of bacteriology

authors： Lee YJ,Prange A,Lichtenberg H,Rohde M,Dashti M,Wiegel J

更新日期：2007-10-01 00:00:00

abstract：:The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...

journal_title：Journal of bacteriology

authors： Volk WA

更新日期：1968-03-01 00:00:00

abstract：:Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...

journal_title：Journal of bacteriology

authors： Roy S,Ajitkumar P

更新日期：2005-04-01 00:00:00

abstract：:A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...

journal_title：Journal of bacteriology

authors： Lindum PW,Anthoni U,Christophersen C,Eberl L,Molin S,Givskov M

更新日期：1998-12-01 00:00:00

abstract：:Spontaneous mutants of Staphylococcus aureus resistant to rifampin, rifamycin SV, streptovaricin, or streptolydigin were isolated and shown to be resistant due to chromosomal rather than plasmid mutations. Based on data concerning spontaneous mutation rates, genetic cotransduction rates, and in vitro sensitivity studi...

journal_title：Journal of bacteriology

authors： Morrow TO,Harmon SA

更新日期：1979-01-01 00:00:00

abstract：:Streptomyces coelicolor Müller contains two superoxide dismutases (SODs), nickel-containing (NiSOD) and iron- and zinc-containing SOD (FeZnSOD). The sodF gene encoding FeZnSOD was isolated by using PCR primers corresponding to the N-terminal peptide sequence of the purified FeZnSOD and a C-terminal region conserved am...

journal_title：Journal of bacteriology

authors： Kim EJ,Chung HJ,Suh B,Hah YC,Roe JH

更新日期：1998-04-01 00:00:00

abstract：:A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The alpha-hemol...

journal_title：Journal of bacteriology

authors： Haque RU

更新日期：1967-02-01 00:00:00

abstract：:Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoin to urea. As a result, these activities increase only 4- to 8-fold upon induction, whereas the urea-degrading enzymes, urea carboxylase a...

journal_title：Journal of bacteriology

authors： Zacharski CA,Cooper TG

更新日期：1978-08-01 00:00:00

abstract：:We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...

journal_title：Journal of bacteriology

authors： Zuberi AR,Ying CW,Parker HM,Ordal GW

更新日期：1990-12-01 00:00:00

abstract：:A group of proteic toxin-antitoxin (TA) cassettes whose representatives are widely distributed among bacterial genomes has been identified. These cassettes occur in chromosomes, plasmids, bacteriophages, and noncomposite transposons, as well as in the SXT conjugative element of Vibrio cholerae. The following four homo...

journal_title：Journal of bacteriology

authors： Dziewit L,Jazurek M,Drewniak L,Baj J,Bartosik D

更新日期：2007-03-01 00:00:00

abstract：:Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The in...

journal_title：Journal of bacteriology

authors： Quirk PG,Dunkley EA Jr,Lee P,Krulwich TA

更新日期：1993-02-01 00:00:00

abstract：:To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

journal_title：Journal of bacteriology

authors： Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

更新日期：1997-11-01 00:00:00

abstract：:The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are controlled by a pathway consisting of cAMP and the virulence factor regulator (Vfr). ...

journal_title：Journal of bacteriology

authors： Berry A,Han K,Trouillon J,Robert-Genthon M,Ragno M,Lory S,Attrée I,Elsen S

更新日期：2018-05-24 00:00:00

abstract：:Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is...

journal_title：Journal of bacteriology

authors： Templeton BA,Savageau MA

更新日期：1974-03-01 00:00:00