Identification of a putative Bacillus subtilis rho gene.

Abstract:

:Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The insertional site was near the beginning of the open reading frame, which was located in a region of the B. subtilis chromosome near the spoOF gene; new sequence data for several open reading frames surrounding the putative rho gene are presented. The predicted B. subtilis Rho protein would have 427 amino acids and a molecular weight of 48,628. The growth of the mutant strain was less than that of the wild type on defined medium at 30 degrees C. On yeast extract-supplemented medium, the growth of MS119 was comparable to that of the wild type on defined medium at 30 degrees C. On yeast extract-supplemented medium, the growth of MS119 was comparable to that of the wild type at 30 degrees C but was much slower at lower temperatures; sporulation occurred and competence was developed in cells of the mutant grown at 30 degrees C. To determine whether the protonophore resistance and sensitivity to low growth temperature resulted from the insertion, a chloramphenicol resistance cassette was inserted into the wild-type B. subtilis rho gene of strain BD170; the resulting derivative displayed the same phenotype as MS119.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Quirk PG,Dunkley EA Jr,Lee P,Krulwich TA

doi

10.1128/jb.175.3.647-654.1993

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

647-54

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

杂志文章
  • Preferential inhibition of plasmid replication in vivo by altered DNA gyrase activity in Escherichia coli.

    abstract::The thermosensitive growth phenotype exerted by runaway-mutant plasmids was suppressed by sublethal doses of the DNA gyrase inhibitors novobiocin or nalidixic acid, although the latter drug was less efficient. A novobiocin-resistant gyrB mutant Escherichia coli strain prevented expression of the runaway phenotype at 3...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.855-857.1985

    authors: Uhlin BE,Nordström K

    更新日期:1985-05-01 00:00:00

  • Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations.

    abstract::Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.5.1554-1567.1992

    authors: Lam HM,Tancula E,Dempsey WB,Winkler ME

    更新日期:1992-03-01 00:00:00

  • Genetics and biochemistry of pyrimidine biosynthesis in Bacillus subtilis: linkage between mutations resulting in a requirement for uracil.

    abstract::A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipien...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.577-581.1973

    authors: Kelleher RJ Jr,Gooder H

    更新日期:1973-11-01 00:00:00

  • Structure, serological specificity, and synthesis of artificial glycoconjugates representing the genus-specific lipopolysaccharide epitope of Chlamydia spp.

    abstract::The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.6.1862-1866.1991

    authors: Holst O,Brade L,Kosma P,Brade H

    更新日期:1991-03-01 00:00:00

  • Overproduction of the AlgT Sigma Factor Is Lethal to Mucoid Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa isolates from chronic lung infections often overproduce alginate, giving rise to the mucoid phenotype. Isolation of mucoid strains from chronic lung infections correlates with a poor patient outcome. The most common mutation that causes the mucoid phenotype is called mucA22 and results in a trun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00445-20

    authors: Cross AR,Raghuram V,Wang Z,Dey D,Goldberg JB

    更新日期:2020-09-23 00:00:00

  • Inactivation of Lgt allows systematic characterization of lipoproteins from Listeria monocytogenes.

    abstract::Lipoprotein anchoring in bacteria is mediated by the prolipoprotein diacylglyceryl transferase (Lgt), which catalyzes the transfer of a diacylglyceryl moiety to the prospective N-terminal cysteine of the mature lipoprotein. Deletion of the lgt gene in the gram-positive pathogen Listeria monocytogenes (i) impairs intra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00976-06

    authors: Baumgärtner M,Kärst U,Gerstel B,Loessner M,Wehland J,Jänsch L

    更新日期:2007-01-01 00:00:00

  • Membrane association of conjugally transferred deoxyribonucleic acid in Escherichia coli minicells.

    abstract::The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.626-633.1971

    authors: Shull FW Jr,Fralick JA,Stratton LP,Fisher WD

    更新日期:1971-05-01 00:00:00

  • Lactate metabolism by Veillonella parvula.

    abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.999-1005.1971

    authors: Ng SK,Hamilton IR

    更新日期:1971-03-01 00:00:00

  • Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5' sequences required for high-level expression.

    abstract::Expression of the Escherichia coli proU operon, which encodes an efficient uptake system for the osmoprotectant glycine betaine, is strongly increased in cells grown at high osmolarity. We isolated 182 independent spontaneous mutants with elevated expression of the chromosomal phi(proV-lacZ) (Hyb2) fusion at low osmol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.801-809.1991

    authors: Lucht JM,Bremer E

    更新日期:1991-01-01 00:00:00

  • Draft genome sequence of Lactobacillus gigeriorum CRBIP 24.85T, isolated from a chicken crop.

    abstract::We report the draft genome of the strain Lactobacillus gigeriorum CRBIP 24.85(T), isolated from a chicken crop. The total length of the 60 scaffolds is about 1.9 Mb, with a GC content of 38% and 2,062 protein-coding sequences (CDS). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01427-12

    authors: Cousin S,Ma L,Creno S,Clermont D,Loux V,Bizet C,Bouchier C

    更新日期:2012-11-01 00:00:00

  • Acid-base titration of streptococci and the physical states of intracellular ions.

    abstract::Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.491-498.1973

    authors: Marquis RE,Porterfield N,Matsumura P

    更新日期:1973-05-01 00:00:00

  • Interchangeability and specificity of components from the quorum-sensing regulatory systems of Vibrio fischeri and Pseudomonas aeruginosa.

    abstract::Autoinduction is a conserved mechanism of cell density-dependent gene regulation that occurs in a variety of gram-negative bacteria. Autoinducible luminescence in Vibrio fischeri requires a transcriptional activator, LuxR, while a LuxR homolog, LasR, activates elastase expression in Pseudomonas aeruginosa. Both LuxR a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.3076-3080.1994

    authors: Gray KM,Passador L,Iglewski BH,Greenberg EP

    更新日期:1994-05-01 00:00:00

  • Clonal diversity and host distribution in Bordetella bronchiseptica.

    abstract::A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2793-2803.1987

    authors: Musser JM,Bemis DA,Ishikawa H,Selander RK

    更新日期:1987-06-01 00:00:00

  • Quorum sensing controls exopolysaccharide production in Sinorhizobium meliloti.

    abstract::Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S. meliloti, of at least one of the two symbiotically important exopolysaccharides, succinoglycan and EPS II. We have previously shown that the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.325-331.2003

    authors: Marketon MM,Glenn SA,Eberhard A,González JE

    更新日期:2003-01-01 00:00:00

  • Identification of phthiodiolone ketoreductase, an enzyme required for production of mycobacterial diacyl phthiocerol virulence factors.

    abstract::Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.14.4760-4766.2005

    authors: Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

    更新日期:2005-07-01 00:00:00

  • Poly-beta-hydroxybutyrate membrane structure and its relationship to genetic transformability in Escherichia coli.

    abstract::The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.553-562.1986

    authors: Reusch RN,Hiske TW,Sadoff HL

    更新日期:1986-11-01 00:00:00

  • Regulated nitrate transport in the cyanobacterium Anacystis nidulans.

    abstract::Intracellular accumulation of nitrate, indicative of the operation of an active nitrate transport system, has been measured in intact cells of the cyanobacterium Anacystis nidulans. The ability of the cells to accumulate nitrate was effectively hindered by either ammonium addition or selective inhibition of CO2 fixati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4376-4378.1987

    authors: Lara C,Romero JM,Guerrero MG

    更新日期:1987-09-01 00:00:00

  • RNase III processing of intervening sequences found in helix 9 of 23S rRNA in the alpha subclass of Proteobacteria.

    abstract::We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.17.4719-4729.2000

    authors: Evguenieva-Hackenberg E,Klug G

    更新日期:2000-09-01 00:00:00

  • Phenotypic suppression of phosphofructokinase mutations in Escherichia coli by constitutive expression of the glyoxylate shunt.

    abstract::Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.3.1090-1100.1974

    authors: Vinopal RT,Fraenkel DG

    更新日期:1974-06-01 00:00:00

  • Presence of multiple sites containing polar material in spherical Escherichia coli cells that lack MreB.

    abstract::In rod-shaped bacteria, certain proteins are specifically localized to the cell poles. The nature of the positional information that leads to the proper localization of these proteins is unclear. In a screen for factors required for the localization of the Shigella sp. actin assembly protein IcsA to the bacterial pole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.17.6187-6196.2005

    authors: Nilsen T,Yan AW,Gale G,Goldberg MB

    更新日期:2005-09-01 00:00:00

  • Effects of mutations to streptomycin resistance on the rate of translation of mutant genetic information.

    abstract::Gartner, T. K. (University of California, Santa Barbara), and E. Orias. Effects of mutations to streptomycin resistance on the rate of translation of mutant genetic information. J. Bacteriol. 91:1021-1028. 1966.-The effects of mutations to streptomycin resistance of independent origin upon the translation of suppressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1021-1028.1966

    authors: Gartner TK,Orias E

    更新日期:1966-03-01 00:00:00

  • Inhibition of staphylococcal biofilm formation by nitrite.

    abstract::Several environmental stresses have been demonstrated to increase polysaccharide intercellular adhesin (PIA) synthesis and biofilm formation by the human pathogens Staphylococcus aureus and Staphylococcus epidermidis. In this study we characterized an adaptive response of S. aureus SA113 to nitrite-induced stress and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00598-07

    authors: Schlag S,Nerz C,Birkenstock TA,Altenberend F,Götz F

    更新日期:2007-11-01 00:00:00

  • Adenosine 5'-triphosphate synthesis energized by an artificially imposed membrane potential in membrane vesicles of Escherichia coli.

    abstract::Adenosine 5'-triphosphate (ATP) synthesis driven by an artificially imposed membrane potential in right-side-out membrane vesicles of Escherichia coli was investigated. Membrane vesicles prepared in the presence of adenosine diphosphate were loaded with K+ by incubation with 0.5 M potassium phosphate. Addition of vali...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.154-161.1976

    authors: Tsuchiya T,Rosen BP

    更新日期:1976-07-01 00:00:00

  • Francisella novicida CRISPR-Cas Systems Can Functionally Complement Each Other in DNA Defense while Providing Target Flexibility.

    abstract::CRISPR-Cas systems are prokaryotic adaptive immune systems that facilitate protection of bacteria and archaea against infection by external mobile genetic elements. The model pathogen Francisella novicida encodes a CRISPR-Cas12a (FnoCas12a) system and a CRISPR-Cas9 (FnoCas9) system, the latter of which has an addition...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00670-19

    authors: Ratner HK,Weiss DS

    更新日期:2020-05-27 00:00:00

  • Isolation and characterization of the cis-trans-unsaturated fatty acid isomerase of Pseudomonas oleovorans GPo12.

    abstract::Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3256-3261.1999

    authors: Pedrotta V,Witholt B

    更新日期:1999-05-01 00:00:00

  • Proline-dependent regulation of Clostridium difficile Stickland metabolism.

    abstract::Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01492-12

    authors: Bouillaut L,Self WT,Sonenshein AL

    更新日期:2013-02-01 00:00:00

  • Active transport of manganese in isolated membrane vesicles of Bacillus subtilis.

    abstract::Membrane vesicles isolated from cells of bacillus subtilis W23 accumulate manganese in the presence of an energy source. The artificial electron donor system ascorbate and phenazine methosulfate or reduced nicotinamide adenine dinucleotide and phenazine methosulfate can supply the energy for the uptake. D-Lactate in t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.123-127.1975

    authors: Bhattacharyya P

    更新日期:1975-07-01 00:00:00

  • Identification of Agrobacterium tumefaciens genes that direct the complete catabolism of octopine.

    abstract::Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.7.1872-1880.1996

    authors: Cho K,Fuqua C,Martin BS,Winans SC

    更新日期:1996-04-01 00:00:00

  • 1,3-Propanediol dehydrogenase from Klebsiella pneumoniae: decameric quaternary structure and possible subunit cooperativity.

    abstract::Klebsiella pneumoniae is a nosocomial pathogen frequently isolated from opportunistic infections, especially in clinical environments. In spite of its potential pathogenicity, this microorganism has several metabolic potentials that could be used in biotechnology applications. K. pneumoniae is able to metabolize glyce...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01077-08

    authors: Marçal D,Rêgo AT,Carrondo MA,Enguita FJ

    更新日期:2009-02-01 00:00:00

  • Tungsten and molybdenum regulation of formate dehydrogenase expression in Desulfovibrio vulgaris Hildenborough.

    abstract::Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00042-11

    authors: da Silva SM,Pimentel C,Valente FM,Rodrigues-Pousada C,Pereira IA

    更新日期:2011-06-01 00:00:00