Abstract:
:Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The insertional site was near the beginning of the open reading frame, which was located in a region of the B. subtilis chromosome near the spoOF gene; new sequence data for several open reading frames surrounding the putative rho gene are presented. The predicted B. subtilis Rho protein would have 427 amino acids and a molecular weight of 48,628. The growth of the mutant strain was less than that of the wild type on defined medium at 30 degrees C. On yeast extract-supplemented medium, the growth of MS119 was comparable to that of the wild type on defined medium at 30 degrees C. On yeast extract-supplemented medium, the growth of MS119 was comparable to that of the wild type at 30 degrees C but was much slower at lower temperatures; sporulation occurred and competence was developed in cells of the mutant grown at 30 degrees C. To determine whether the protonophore resistance and sensitivity to low growth temperature resulted from the insertion, a chloramphenicol resistance cassette was inserted into the wild-type B. subtilis rho gene of strain BD170; the resulting derivative displayed the same phenotype as MS119.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Quirk PG,Dunkley EA Jr,Lee P,Krulwich TAdoi
10.1128/jb.175.3.647-654.1993subject
Has Abstractpub_date
1993-02-01 00:00:00pages
647-54issue
3eissn
0021-9193issn
1098-5530journal_volume
175pub_type
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更新日期:1994-05-01 00:00:00
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doi:10.1128/jb.169.6.2793-2803.1987
更新日期:1987-06-01 00:00:00
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更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.168.2.553-562.1986
更新日期:1986-11-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.169.9.4376-4378.1987
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更新日期:2007-11-01 00:00:00
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更新日期:1999-05-01 00:00:00
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更新日期:1975-07-01 00:00:00
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更新日期:1996-04-01 00:00:00
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更新日期:2009-02-01 00:00:00
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更新日期:2011-06-01 00:00:00