Abstract:
:Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the catabolism of octopine or its metabolites, were obtained. One group of mutants could not use octopine as a carbon source, while a second group of mutants could not utilize arginine or ornithine and a third group could not utilize octopine, arginine, ornithine, or proline as a carbon source. Utilization of these compounds as nitrogen sources showed similar but not identical patterns. Fifteen fusions were subcloned together with adjacent DNA. Sequence analysis and further genetic analysis indicated that insertions of the first group are localized in the occ region of the Ti plasmid. Insertions of the second group were localized to a gene encoding ornithine cyclodeaminase. This gene is very similar to, but distinct from, a homolog located on the Ti plasmid. This gene is located immediately downstream from a gene encoding an arginase. Genetic experiments indicated that this arginase gene is essential for octopine and arginine catabolism. Insertions of the third group was localized to a gene whose product is required for degradation of proline. We therefore have identified all steps required for the catabolism of octopine to glutamate.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cho K,Fuqua C,Martin BS,Winans SCdoi
10.1128/jb.178.7.1872-1880.1996subject
Has Abstractpub_date
1996-04-01 00:00:00pages
1872-80issue
7eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::Vi-agglutinin, active cutaneous anaphylaxis and protective responses (ed(50)) of three mouse strains (CFW, NIH, and Balb/cAnN) to acetone-inactivated typhoid vaccine and soluble Vi antigen were compared. Seven days after immunization with either typhoid vaccine or Vi antigen the three strains of mice differed with res...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.8-12.1969
更新日期:1969-07-01 00:00:00
abstract::Salmonella enterica can utilize fructose-asparagine (F-Asn) as a source of carbon and nitrogen. This capability has been attributed to five genes in the fra locus. Previously, we determined that mutations in fraB (deglycase), fraD (kinase), or fraA (transporter) eliminated the ability of Salmonella to grow on F-Asn, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00330-17
更新日期:2017-10-17 00:00:00
abstract::We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.621-628.2002
更新日期:2002-02-01 00:00:00
abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01619-06
更新日期:2007-04-01 00:00:00
abstract::AppppA and the DnaK protein have both been hypothesized to function in regulating the heat shock response of Escherichia coli. The proposals are that AppppA serves as a signal (alarmone) to turn on the heat shock response, whereas the DnaK protein is necessary to turn off the heat shock response. A simple model would ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.931-935.1986
更新日期:1986-11-01 00:00:00
abstract::Rhodobacter capsulatus mutants unable to fix nitrogen were isolated by random transposon Tn5 mutagenesis. The Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomal regions designated A, B, and C. The majority of Tn5 insertions (21 mutants) map within nif region A, characterized by two Cl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.693-699.1988
更新日期:1988-02-01 00:00:00
abstract::2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The purified enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 205 kDa by gel filtration chromatography. Thus, the homotetramer ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.20.6488-6494.1997
更新日期:1997-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.12.4077-4085.2005
更新日期:2005-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.1050-1063.1982
更新日期:1982-03-01 00:00:00
abstract::In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05128-11
更新日期:2011-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.18.6370-6378.2005
更新日期:2005-09-01 00:00:00
abstract::The 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown Peptostreptococcus productus Marburg was purified to apparent homogeneity. The purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with NADP+ as the electron acceptor at a specific activity of 627 U/mg of protein. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.4.1414-1419.1991
更新日期:1991-02-01 00:00:00
abstract::Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.409-416.1973
更新日期:1973-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.819-823.1971
更新日期:1971-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01695-09
更新日期:2010-06-01 00:00:00
abstract::We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.13.4356-4360.1992
更新日期:1992-07-01 00:00:00
abstract::Glutathione and soluble thiol content were examined in a broad spectrum of bacteria. Significant soluble thiol was present in all cases. The thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. Glutathione was absent in four anerobes and one microa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1126-1129.1978
更新日期:1978-03-01 00:00:00
abstract::A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.2093-2100.2001
更新日期:2001-03-01 00:00:00
abstract::After the demonstration that Stigmatella aurantiaca DW4 secretes an endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans (S. Bourgerie, Y. Karamanos, T. Grard, and R. Julien, J. Bacteriol. 176:6170-6174, 1994), an ENGase activity having the same substrate specifi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.4.916-920.1995
更新日期:1995-02-01 00:00:00
abstract::In the yeast Saccharomyces cerevisiae, IRE1 encodes a bifunctional protein with transmembrane kinase and endoribonuclease activities. HAC1 encodes a transcription factor which has a basic leucine zipper domain. Both gene products play a crucial role in the unfolded protein response. Mutants in which one of these genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.17.4985-4993.2001
更新日期:2001-09-01 00:00:00
abstract::Hancock, R. (Harvard Medical School, Boston, Mass.). Early effects of streptomycin on Bacillus megaterium. J. Bacteriol. 88:633-639. 1964.-The sequence of some early effects of streptomycin on Bacillus megaterium was investigated. An increase in the rate of efflux of cellular K ions preceded loss of viability, but eff...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.633-639.1964
更新日期:1964-09-01 00:00:00
abstract::The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.4818-4824.1999
更新日期:1999-08-01 00:00:00
abstract::Nucleoside diphosphate kinase (Ndk) is a ubiquitous enzyme which functions in balancing the nucleotide pool of the cell. We have recently reported that in addition to being intracellular in both mucoid and nonmucoid Pseudomonas aeruginosa, Ndk is also secreted into the extracellular environment by mucoid P. aeruginosa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.13.3826-3831.2000
更新日期:2000-07-01 00:00:00
abstract::Zorbamycin (U-30,604E) induces rapid degradation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in Bacillus subtilis cells. DNA degradation is initiated first and is closely followed by the degradation of RNA. No interaction between isolated DNA and zorbamycin is observed. Nucleic acid and protein syntheses...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.30-37.1971
更新日期:1971-10-01 00:00:00
abstract::Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many labor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00141-12
更新日期:2012-05-01 00:00:00
abstract::A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the eff...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.306-311.1984
更新日期:1984-07-01 00:00:00
abstract::Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.14.4382-4385.2001
更新日期:2001-07-01 00:00:00
abstract::Oxalurate, the gratuitous inducer of the allantoin degradative enzymes, was taken into the cell by an energy-dependent active transport system with an apparent Km of 1.2 mM. Efflux of previously accumulated oxalurate was rapid, with a half-life of about 2 min. The oxalurate uptake system appears to be both constitutiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.917-923.1979
更新日期:1979-09-01 00:00:00
abstract::A Bacillus subtilis sigM null mutant, lacking the extracytoplasmic function sigma(M) protein, was sensitive to paraquat (PQ), a superoxide-generating reagent, but not to the redox stress-inducing compounds hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, or diamide. Surprisingly, a sigM mutant was only ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.2948-2956.2005
更新日期:2005-05-01 00:00:00
abstract::Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsatu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6623-6627.1996
更新日期:1996-11-01 00:00:00