Acid-base titration of streptococci and the physical states of intracellular ions.

Abstract:

:Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of the intracellular Mg(2+) ions appeared to be firmly bound to stable internal cell components but could be displaced reversibly by hydronium ions. The cell membrane acted as a barrier to ion movements, and Mg(2+) displacement from intact cells required more acid conditions, by as much as 2.5 pH units, than did displacement from butanol-damaged cells. Some 15 to 20% of the cell magnesium appeared to be associated with surface structures in that it could be removed at pH 7 with ethylenediaminetetraacetic acid or displaced by Co(2+), Ni(2+), Sr(2+), or La(3+). Magnesium could be displaced from isolated cell walls and membranes by hydronium ions in the pH range from 5 to 3, over which carboxyl groups were titrated. Displacement of magnesium from ribosomes also took place between pH 5 and 3, but it was more difficult to identify the magnesium-releasing groups because both protein carboxyl groups and purine and pyrimidine ring nitrogens can become protonated in this pH range. Isolated protoplast membranes remained structurally intact when completely depleted of magnesium. Furthermore, protoplasts isolated from intact cells were found to have greatly enhanced resistance to osmotic shock in acid media, even when solute loss was not extensive. Osmotic resistance was lost when the protoplasts were again placed in neutral media, and this reversibility suggested that acidification caused changes in the physical properties of membranes as well as solute leakage from cells.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Marquis RE,Porterfield N,Matsumura P

doi

10.1128/JB.114.2.491-498.1973

subject

Has Abstract

pub_date

1973-05-01 00:00:00

pages

491-8

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

114

pub_type

杂志文章
  • Growth of mycobacteria on carbon monoxide and methanol.

    abstract::Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.142-147.2003

    authors: Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

    更新日期:2003-01-01 00:00:00

  • Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis.

    abstract::Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.4.793-796.1962

    authors: FUKUYAMA TT,O'KANE DJ

    更新日期:1962-10-01 00:00:00

  • Analysis of putative Chlamydia trachomatis chaperones Scc2 and Scc3 and their use in the identification of type III secretion substrates.

    abstract::The obligate intracellular pathogen Chlamydia trachomatis expresses a type III secretion system (T3SS) which has the potential to contribute significantly to pathogenesis. Based on a demonstrated role of type III secretion (T3S)-specific chaperones in the secretion of antihost proteins by gram-negative pathogens, we i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.18.6466-6478.2005

    authors: Fields KA,Fischer ER,Mead DJ,Hackstadt T

    更新日期:2005-09-01 00:00:00

  • Activation of the σE-dependent stress pathway by conjugative TraR may anticipate conjugational stress.

    abstract::Horizontal gene transfer by conjugation plays a major role in bacterial evolution, allowing the acquisition of new traits, such as virulence and resistance to antibacterial agents. With the increased antibiotic resistance in bacterial pathogens, a better understanding of how bacteria modulate conjugation under changin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02279-14

    authors: Grace ED,Gopalkrishnan S,Girard ME,Blankschien MD,Ross W,Gourse RL,Herman C

    更新日期:2015-03-01 00:00:00

  • Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1.

    abstract::Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugates obtained after mixed i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.835-843.1982

    authors: LeBlanc DJ,Lee LN

    更新日期:1982-05-01 00:00:00

  • Identification of a putative Bacillus subtilis rho gene.

    abstract::Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.3.647-654.1993

    authors: Quirk PG,Dunkley EA Jr,Lee P,Krulwich TA

    更新日期:1993-02-01 00:00:00

  • The Acinetobacter baylyi Hfq gene encodes a large protein with an unusual C terminus.

    abstract::In gammaproteobacteria the Hfq protein shows a great variation in size, especially in its C-terminal part. Extremely large Hfq proteins consisting of almost 200 amino acid residues and more are found within the gammaproteobacterial family Moraxellaceae. The difference in size compared to other Hfq proteins is due to a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00490-09

    authors: Schilling D,Gerischer U

    更新日期:2009-09-01 00:00:00

  • Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli.

    abstract::The DNA sequence of a 3.23-kilobase fragment of the Escherichia coli chromosome encoding biosynthetic arginine decarboxylase (ADC) was determined. This sequence contained the speA open reading frame (ORF) as well as partial speB and metK ORFs. The ADC ORF is 1,974 nucleotides long; the deduced polypeptide contains 658...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4631-4640.1990

    authors: Moore RC,Boyle SM

    更新日期:1990-08-01 00:00:00

  • An extraintestinal, pathogenic isolate of Escherichia coli (O4/K54/H5) can produce a group 1 capsule which is divergently regulated from its constitutively produced group 2, K54 capsular polysaccharide.

    abstract::We are studying an O4/K54/H5 Escherichia coli bacteremic isolate (CP9) as a model pathogen for extraintestinal infection. Its group 2, K54 capsular polysaccharide is an important virulence determinant and confers serum resistance. In this study the effect of the group 1 capsule regulators, RcsA, RcsB, and Lon protease...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.23.7617-7623.1993

    authors: Russo TA,Singh G

    更新日期:1993-12-01 00:00:00

  • The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.

    abstract::Single-celled organisms must survive exposure to environmental extremes. Perhaps one of the most variable and potentially life-threatening changes that can occur is that of a rapid and acute decrease in external osmolarity. This easily translates into several atmospheres of additional pressure that can build up within...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00576-12

    authors: Booth IR,Blount P

    更新日期:2012-09-01 00:00:00

  • Distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species.

    abstract::The distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species was examined. Enzyme activity was demonstrated in cell-free growth media from all members of the family Vibrionaceae which were tested except Plesiomonas shigelloides. In each case, enzyme was produced in exponential to e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.1.132-136.1979

    authors: MacIntyre S,Trust TJ,Buckley JT

    更新日期:1979-07-01 00:00:00

  • Identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in Staphylococcus aureus.

    abstract::A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.11.3197-3203.2000

    authors: McNamara PJ,Milligan-Monroe KC,Khalili S,Proctor RA

    更新日期:2000-06-01 00:00:00

  • DRUG RESISTANCE OF ENTERIC BACTERIA. II. TRANSDUCTION OF TRANSMISSIBLE DRUG-RESISTANCE (R) FACTORS WITH PHAGE EPSILON.

    abstract::Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. II. Transduction of transmissible drug-resistance (R) factors with phage epsilon. J. Bacteriol. 86:1332-1338. 1963.-Transmissible drug-resistance (R) factors, which transfer resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1332-1338.1963

    authors: HARADA K,KAMEDA M,SUZUKI M,MITSUHASHI S

    更新日期:1963-12-01 00:00:00

  • Functional characterization of a novel xylanase from a corn strain of Erwinia chrysanthemi.

    abstract::A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.2093-2100.2001

    authors: Hurlbert JC,Preston JF 3rd

    更新日期:2001-03-01 00:00:00

  • A novel activity in Escherichia coli K-12 that directs restriction of DNA modified at CG dinucleotides.

    abstract::The restriction systems McrA and McrB of Escherichia coli K-12 are known to attack DNA containing modified cytosine. In strains lacking both activities, however, we observed that DNA methylated at CG dinucleotides (as is mammalian DNA) was still significantly restricted. We show that this substantial barrier to the ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.16.5220-5223.1991

    authors: Kelleher JE,Raleigh EA

    更新日期:1991-08-01 00:00:00

  • Regulation of cell size in the yeast Saccharomyces cerevisiae.

    abstract::For cells of the yeast Saccharomyces cerevisiae, the size at initiation of budding is proportional to growth rate for rates from 0.33 to 0.23 h-1. At growth rates lower than 0.23 h-1, cells displayed a minimum cell size at bud initiation independent of growth rate. Regardless of growth rate, cells displayed an increas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.1-5.1979

    authors: Johnston GC,Ehrhardt CW,Lorincz A,Carter BL

    更新日期:1979-01-01 00:00:00

  • Secretion of methanol-insoluble heat-stable enterotoxin (STB): energy- and secA-dependent conversion of pre-STB to an intermediate indistinguishable from the extracellular toxin.

    abstract::The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2427-2432.1990

    authors: Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

    更新日期:1990-05-01 00:00:00

  • Genetic analysis of bacteriophage lambda cIII gene: mRNA structural requirements for translation initiation.

    abstract::The bacteriophage lambda cIII gene product regulates the lysogenic pathway. The cIII gene is located in the leftward operon, which is transcribed from the pL promoter. We have previously shown (S. Altuvia and A. B. Oppenheim, J. Bacteriol. 167:415-419, 1986) that mutations that show elevated expression lie within the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2563-2572.1989

    authors: Kornitzer D,Teff D,Altuvia S,Oppenheim AB

    更新日期:1989-05-01 00:00:00

  • General stress transcription factor sigmaB and its role in acid tolerance and virulence of Listeria monocytogenes.

    abstract::The gene encoding the general stress transcription factor sigmaB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification. Evidence for gene identification includes the following: (i) phylogenetic analyses of reported amino acid sequences for ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.14.3650-3656.1998

    authors: Wiedmann M,Arvik TJ,Hurley RJ,Boor KJ

    更新日期:1998-07-01 00:00:00

  • Transcriptional phase variation of a type III restriction-modification system in Helicobacter pylori.

    abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6615-6624.2002

    authors: de Vries N,Duinsbergen D,Kuipers EJ,Pot RG,Wiesenekker P,Penn CW,van Vliet AH,Vandenbroucke-Grauls CM,Kusters JG

    更新日期:2002-12-01 00:00:00

  • The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.

    abstract::Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.24.7397-7402.2001

    authors: Thanedar S,Dineshkumar TK,Varshney U

    更新日期:2001-12-01 00:00:00

  • DETECTION OF ENTEROPATHOGENIC ESCHERICHIA COLI IN FECAL CULTURES BY USE OF A MODIFIED FLUORESCENT-ANTIBODY TECHNIQUE.

    abstract::Martin, Alice J. (Nassau County Department of Health, Hempstead, N.Y.), and Margaret O'Brien. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bacteriol. 89:570-573. 1965.-The application of the fluorescent-antibody (FA) technique to cultures of f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.570-573.1965

    authors: MARTIN AJ,O'BRIEN M

    更新日期:1965-03-01 00:00:00

  • Dual negative control of spx transcription initiation from the P3 promoter by repressors PerR and YodB in Bacillus subtilis.

    abstract::The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01520-06

    authors: Leelakriangsak M,Kobayashi K,Zuber P

    更新日期:2007-03-01 00:00:00

  • Global gene expression analysis of the heat shock response in the phytopathogen Xylella fastidiosa.

    abstract::Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00182-06

    authors: Koide T,Vêncio RZ,Gomes SL

    更新日期:2006-08-01 00:00:00

  • Stereochemical course of two arene-cis-diol dehydrogenases specifically induced in Pseudomonas putida.

    abstract::Catabolism of nonphenolic arenes is frequently initiated by dioxygenases, yielding single isomer products with two adjacent hydroxylated asymmetric centers. The next enzymic reaction dehydrogenates these cyclic cis-diols, with aromatization yielding catechols for ring cleavage. There are two stereochemical questions t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.4023-4029.1997

    authors: Morawski B,Casy G,Illaszewicz C,Griengl H,Ribbons DW

    更新日期:1997-06-01 00:00:00

  • Proteinase C (carboxypeptidase Y) mutant of yeast.

    abstract::A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1150-1156.1975

    authors: Wolf DH,Fink GR

    更新日期:1975-09-01 00:00:00

  • Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms.

    abstract::Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.2.527-534.1981

    authors: Kawamoto I,Oka T,Nara T

    更新日期:1981-05-01 00:00:00

  • Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.

    abstract::Using physical and genetic data, we have demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasmid pRmeSU47a. This plasmid includes four loci involved in exopolysaccharide (exo) synthesis as well as two loci involved in thiamine biosyn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.66-72.1986

    authors: Finan TM,Kunkel B,De Vos GF,Signer ER

    更新日期:1986-07-01 00:00:00

  • Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli.

    abstract::A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.350-355.1986

    authors: de Lorenzo V,Neilands JB

    更新日期:1986-07-01 00:00:00

  • Functional characterization of SsaE, a novel chaperone protein of the type III secretion system encoded by Salmonella pathogenicity island 2.

    abstract::The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00863-09

    authors: Miki T,Shibagaki Y,Danbara H,Okada N

    更新日期:2009-11-01 00:00:00