Regulation of cell size in the yeast Saccharomyces cerevisiae.

abstract：:Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

journal_title：Journal of bacteriology

authors： Arnold WN,Garrison RG

更新日期：1979-03-01 00:00:00

abstract：:The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...

journal_title：Journal of bacteriology

authors： Kung FC,Raymond J,Glaser DA

更新日期：1976-06-01 00:00:00

abstract：:The gene coding for alveolysin, the thiol-activated toxin produced by Bacillus alvei, has been cloned by means of an oligonucleotide based on the known N-terminal sequence of the secreted protein. The complete nucleotide sequence of the gene has been determined. The deduced amino acid sequence of alveolysin shows that...

journal_title：Journal of bacteriology

authors： Geoffroy C,Mengaud J,Alouf JE,Cossart P

更新日期：1990-12-01 00:00:00

abstract：:Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...

journal_title：Journal of bacteriology

authors： Massa SM,Sharma AD,Siletti C,Tu Z,Godfrey JJ,Gutheil WG,Huynh TN

更新日期：2020-11-19 00:00:00

abstract：:An 11-kbp DNA element of unknown function interrupts the nifD gene in vegetative cells of Anabaena sp. strain PCC 7120. In developing heterocysts the nifD element excises from the chromosome via site-specific recombination between short repeat sequences that flank the element. The nucleotide sequence of the nifH-proxi...

journal_title：Journal of bacteriology

authors： Lammers PJ,McLaughlin S,Papin S,Trujillo-Provencio C,Ryncarz AJ 2nd

更新日期：1990-12-01 00:00:00

abstract：:A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of Streptococcus sanguis. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomuc...

journal_title：Journal of bacteriology

authors： Hayano S,Tanaka A

更新日期：1969-03-01 00:00:00

abstract：:The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

journal_title：Journal of bacteriology

authors： Chou CC,Lin TW,Chen CY,Wang AH

更新日期：2003-07-01 00:00:00

abstract：:Listeria monocytogenes is a facultative intracellular gram-positive bacterium responsible for severe opportunistic infections in humans and animals. We had previously identified a gene encoding a putative UDP-N-acetylglucosamine 2-epimerase, a precursor of the teichoic acid linkage unit, in the genome of L monocytogen...

journal_title：Journal of bacteriology

authors： Dubail I,Bigot A,Lazarevic V,Soldo B,Euphrasie D,Dupuis M,Charbit A

更新日期：2006-09-01 00:00:00

abstract：:The nucleotide sequences of a 3,479-base-pair HindIII DNA fragment from Bacillus sphaericus 2362 and a 2,940-base-pair fragment from strain 2297 were determined; only minor differences were detected between them. Each contained two open reading frames coding for proteins of 51.4 and 41.9 kilodaltons. Both proteins wer...

journal_title：Journal of bacteriology

authors： Baumann L,Broadwell AH,Baumann P

更新日期：1988-05-01 00:00:00

abstract：:Several resistant mutants of a transformable group H streptococcus, strain Challis, were isolated from media containing high concentrations of streptomycin. Mutants SR5a and SR5 exhibited high and low transformability, respectively, when exposed to deoxyribonucleic acid (DNA) from a novobiocin-resistant Challis strain...

journal_title：Journal of bacteriology

authors： Perry D

更新日期：1968-01-01 00:00:00

abstract：:The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene enco...

journal_title：Journal of bacteriology

authors： Griggs DW,Tharp BB,Konisky J

更新日期：1987-12-01 00:00:00

abstract：:Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

journal_title：Journal of bacteriology

authors： MOULDER JW,NOVOSEL DL,TRIBBY II

更新日期：1965-01-01 00:00:00

abstract：:The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...

journal_title：Journal of bacteriology

authors： Wang RJ,Morse HG,Morse ML

更新日期：1970-12-01 00:00:00

abstract：:CluA is a cell surface-presented protein that causes cell aggregation and is essential for a high-efficiency conjugation process in Lactococcus lactis. We know from previous work that in addition to promoting cell-to-cell contact, CluA is involved in sex factor DNA transfer. To define the CluA domains involved in aggr...

journal_title：Journal of bacteriology

authors： Stentz R,Gasson M,Shearman C

更新日期：2006-03-01 00:00:00

abstract：:The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which is mediated by short-chain fatty acids. During this fatty acid-mediated expression of spv, RpoS is required for inducti...

journal_title：Journal of bacteriology

authors： El-Gedaily A,Paesold G,Chen CY,Guiney DG,Krause M

更新日期：1997-02-01 00:00:00

abstract：:Two active lysine transport systems were detected in Saccharomycopsis lipolytica. No excretion of lysine out of the cells could be obtained, even by chasing with L-lysine or by poisoning with sodium azide. The kinetic properties of one of the permeases, the high-affinity lysine permease, were studied in detail. Its Km...

journal_title：Journal of bacteriology

authors： Beckerich JM,Heslot H

更新日期：1978-02-01 00:00:00

abstract：:Acinetobacter baumannii is a Gram-negative bacterium causing nosocomial infections worldwide. To gain quick insight into the molecular basis of biofilm formation in A. baumannii, we determined the complete genome sequence of A. baumannii strain 1656-2, which forms sturdy biofilm and is resistant to multiple drugs. ...

journal_title：Journal of bacteriology

authors： Park JY,Kim S,Kim SM,Cha SH,Lim SK,Kim J

更新日期：2011-11-01 00:00:00

abstract：:A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...

journal_title：Journal of bacteriology

authors： Musser JM,Bemis DA,Ishikawa H,Selander RK

更新日期：1987-06-01 00:00:00

abstract：:In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

journal_title：Journal of bacteriology

authors： Myers CR,Myers JM

更新日期：1992-06-01 00:00:00

abstract：:Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

journal_title：Journal of bacteriology

authors： Goldman BS,Lin JT,Stewart V

更新日期：1994-08-01 00:00:00

abstract：:Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions...

journal_title：Journal of bacteriology

authors： Diaz-Mendoza M,Bideshi DK,Federici BA

更新日期：2012-03-01 00:00:00

abstract：:The major sheath protein-encoding gene (mspA) of the oral spirochete Treponema maltophilum ATCC 51939(T) was cloned by screening a genomic library with an anti-outer membrane fraction antibody. The mspA gene encodes a precursor protein of 575 amino acids with a predicted molecular mass of 62.3 kDa, including a signal ...

journal_title：Journal of bacteriology

authors： Heuner K,Choi BK,Schade R,Moter A,Otto A,Göbel UB

更新日期：1999-02-01 00:00:00

abstract：:The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10 degrees C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstab...

journal_title：Journal of bacteriology

authors： Tanabe H,Goldstein J,Yang M,Inouye M

更新日期：1992-06-01 00:00:00

abstract：:In order to predict the binding regions within the complex formed by Toll-like receptor 5 (TLR-5) and flagellin, a complementary hydropathy between the two proteins was sought. A region common to the flagellins of Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes was shown to ...

journal_title：Journal of bacteriology

authors： Jacchieri SG,Torquato R,Brentani RR

更新日期：2003-07-01 00:00:00

abstract：:Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...

journal_title：Journal of bacteriology

authors： Hamrick TS,Harris SL,Spears PA,Havell EA,Horton JR,Russell PW,Orndorff PE

更新日期：2000-07-01 00:00:00

abstract：:Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...

journal_title：Journal of bacteriology

authors： Baron SF,Franklund CV,Hylemon PB

更新日期：1991-08-01 00:00:00

abstract：:The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...

journal_title：Journal of bacteriology

authors： Stibitz S,Weiss AA,Falkow S

更新日期：1988-07-01 00:00:00

abstract：:Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separa...

journal_title：Journal of bacteriology

authors： Waller LN,Stump MJ,Fox KF,Harley WM,Fox A,Stewart GC,Shahgholi M

更新日期：2005-07-01 00:00:00

abstract：:AdnA is a transcription factor in Pseudomonas fluorescens that affects flagellar synthesis, biofilm formation, and sand adhesion. To identify the AdnA regulon, we used a promoterless Tn5-lacZ element to study the phenotypes of insertion mutants in the presence and absence of AdnA. Of 12,000 insertions, we identified s...

journal_title：Journal of bacteriology

authors： Robleto EA,López-Hernández I,Silby MW,Levy SB

更新日期：2003-01-01 00:00:00

abstract：:In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular m...

journal_title：Journal of bacteriology

authors： Ma JF,Hager PW,Howell ML,Phibbs PV,Hassett DJ

更新日期：1998-04-01 00:00:00