Abstract:
:In order to predict the binding regions within the complex formed by Toll-like receptor 5 (TLR-5) and flagellin, a complementary hydropathy between the two proteins was sought. A region common to the flagellins of Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes was shown to be hydropathically complementary to the 552-to-561 fragment of TLR-5, whose sequence is EILDISRNQL. The hydrophobicity profile of this region is shared with flagellins of 377 bacterial species out of a total of 723 publicly available sequences. A conformational analysis of the predicted binding site of TLR-5, whose structure is still unknown, was carried out with a methodology already applied to similar problems. To sample the conformations available to the peptide chain, a plot of the number of conformations per unit energy interval (density of states) versus energy was built. Following a theoretical argument, conformations belonging to maxima in this plot were selected. The most stable structure obtained in this search, an alpha-helical conformation, was shown to form the electrostatic interactions Glu552-Gln89, Asp555-Arg92, and Arg558-Glu93 with the predicted binding site of the flagellin of S. enterica serovar Typhimurium, formed by the 88-to-97 chain fragment (LQRVRELAVQ), which is likewise alpha helical.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Jacchieri SG,Torquato R,Brentani RRdoi
10.1128/jb.185.14.4243-4247.2003keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
4243-7issue
14eissn
0021-9193issn
1098-5530journal_volume
185pub_type
杂志文章abstract::Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.00925-07
更新日期:2007-12-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.113.2.932-936.1973
更新日期:1973-02-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1999-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2017-06-27 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1999-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.409-416.1973
更新日期:1973-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1993-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.145-152.1980
更新日期:1980-04-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1987-06-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1975-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1971-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1973-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1973-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1983-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.3.740-749.2004
更新日期:2004-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00