Abstract:
:AdnA is a transcription factor in Pseudomonas fluorescens that affects flagellar synthesis, biofilm formation, and sand adhesion. To identify the AdnA regulon, we used a promoterless Tn5-lacZ element to study the phenotypes of insertion mutants in the presence and absence of AdnA. Of 12,000 insertions, we identified seven different putative open reading frames (ORFs) activated by AdnA (named aba for activated by AdnA). aba120 and aba177 showed homology to flgC and flgI, components of the basal body of the flagella in Pseudomonas aeruginosa. Two other insertions, aba18 and aba51, disrupted genes affecting chemotaxis. The mutant loci aba160 (possibly affecting lipopolysaccharide synthesis) and aba175 (unknown function) led to loss of flagella. The mutant bearing aba203 became motile when complemented with adnA, but the mutated gene showed no similarity to known genes. Curiously, aba18, aba51, aba160, and aba203 mutants formed biofilms even in the absence of AdnA, suppressing the phenotype of the adnA deletion mutant. The combined findings suggest that flagella are nonessential for sand attachment or biofilm formation. Sequence and promoter analyses indicate that AdnA affects at least 23 ORFs either directly or by polar effects. These results support the concept that AdnA regulates cell processes other than those directly related to flagellar synthesis and define a broader cadre of genes in P. fluorescens than that described so far for its homolog, FleQ, in P. aeruginosa.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Robleto EA,López-Hernández I,Silby MW,Levy SBdoi
10.1128/jb.185.2.453-460.2003keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
453-60issue
2eissn
0021-9193issn
1098-5530journal_volume
185pub_type
杂志文章abstract::Population density-dependent expression of luminescence in Vibrio fischeri is controlled by the autoinducer N-3-oxohexanoyl-L-homoserine lactone (autoinducer 1 [AI-1]), which via LuxR activates transcription of the lux operon (luxICDABEG, encoding the putative autoinducer synthase [LuxI] and the luminescence enzymes)....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.4.971-976.1996
更新日期:1996-02-01 00:00:00
abstract::Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Lel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1184-1194.2001
更新日期:2001-02-01 00:00:00
abstract::The effects of nickel on the expression of hydrogenase in the hydrogen-oxidizing bacterium Alcaligenes latus were studied. In the absence of added nickel, both hydrogenase activity, measured as O2-dependent H2 uptake, and hydrogenase protein, measured in a Western immunoblot, were very low compared with the levels in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3891-3896.1988
更新日期:1988-09-01 00:00:00
abstract::Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.4875-4882.1994
更新日期:1994-08-01 00:00:00
abstract::A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.13.3673-3679.1995
更新日期:1995-07-01 00:00:00
abstract::The activity of the toluene-responsive sigma(54) Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when ame...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.4.956-960.2000
更新日期:2000-02-01 00:00:00
abstract::Fine details of the internal and external morphology of the yeastlike and mycelial phases of the dimorphic fungal pathogen Sporotrichum schenckii as seen in ultrathin sections are described and illustrated by electronphotomicrography. Comparisons of yeastlike phase ultrastructure were made using two different methods ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1010-1019.1969
更新日期:1969-11-01 00:00:00
abstract::Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00244-11
更新日期:2011-05-01 00:00:00
abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1180-1191.1964
更新日期:1964-05-01 00:00:00
abstract::Physiological studies of a mutant of Escherichia coli lacking the three lytic transglycosylases Slt70, MltA, and MltB revealed that interference with murein turnover can prevent AmpC beta-lactamase induction. The triple mutant, although growing normally, shows a dramatically reduced rate of murein turnover. Despite th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.23.7192-7198.1999
更新日期:1999-12-01 00:00:00
abstract::Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.758-769.1980
更新日期:1980-02-01 00:00:00
abstract::A previously undetected Bacillus subtilis aspartokinase isozyme, which we have called aspartokinase III, has been characterized. The new isozyme was most readily detected in extracts of cells grown with lysine, which repressed aspartokinase II and induced aspartokinase III, or in extracts of strain VS11, a mutant lack...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.218-223.1990
更新日期:1990-01-01 00:00:00
abstract::From the marine sponge Halichondria panicea a lectin was isolated and characterized. The homogeneous lectin (composed of protein to 80.7% and of neutral carbohydrates to 14.1%) had a molecular weight of 78,000 (determined by gel filtration) and consisted of four subunits with a molecular weight of 21,000 each (determi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.548-558.1981
更新日期:1981-01-01 00:00:00
abstract::Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. The invasion process requires the synthesis and secretion of the virulence plasmid-encoded Ipa proteins. Using TnphoA mutagenesis, we have identified two virulence plasmid genes, mxiJ and mxiM, that encode proteins exporte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.23.7661-7669.1992
更新日期:1992-12-01 00:00:00
abstract::Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1469-1475.1968
更新日期:1968-04-01 00:00:00
abstract::Using physical and genetic data, we have demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasmid pRmeSU47a. This plasmid includes four loci involved in exopolysaccharide (exo) synthesis as well as two loci involved in thiamine biosyn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.66-72.1986
更新日期:1986-07-01 00:00:00
abstract::We described previously a class of mutations (phenotypic designation R2(-)) in Staphylococcus aureus that were unlinked to penicillinase genes but nevertheless imposed constitutive synthesis of this enzyme upon wild-type plasmids. The plasmids affected by these mutations have been found to possess the same maintenance...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.3.616-621.1970
更新日期:1970-09-01 00:00:00
abstract::Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.106-111.1962
更新日期:1962-01-01 00:00:00
abstract::Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.310-318.1969
更新日期:1969-10-01 00:00:00
abstract::While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00160-07
更新日期:2007-06-01 00:00:00
abstract::The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.7.2298-2305.2001
更新日期:2001-04-01 00:00:00
abstract::The tetracycline resistance element of plasmid pBR322 partially complements the potassium transport defect of Escherichia coli K-12 mutants having markedly impaired K+ transport. The plasmid increases K+ transport. The Tn10 element does not result in increased transport, demonstrating that the effect is not general fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.1188-1190.1984
更新日期:1984-12-01 00:00:00
abstract::Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.425-427.1980
更新日期:1980-10-01 00:00:00
abstract::Legionella pneumophila expresses two catalase-peroxidase enzymes that exhibit strong peroxidatic but weak catalatic activities, suggesting that other enzymes participate in decomposition of hydrogen peroxide (H2O2). Comparative genomics revealed that L. pneumophila and its close relative Coxiella burnetii each contain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00635-06
更新日期:2006-09-01 00:00:00
abstract::Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsatu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6623-6627.1996
更新日期:1996-11-01 00:00:00
abstract::Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00628-12
更新日期:2012-08-01 00:00:00
abstract::Comparative genome analysis of Enterococcus faecium recently revealed that a genomic island containing the esp gene, referred to as the esp-containing pathogenicity island (esp PAI), can be transferred by conjugation and contains a partial Tn916-like element and an integrase gene, intA. Here, we characterize the role ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00952-10
更新日期:2011-02-01 00:00:00
abstract::Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.712-720.1969
更新日期:1969-05-01 00:00:00
abstract::The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported on a direct cooperation. To investigate the possible interaction of the putative RNA helicases CshA and CshB and the CSP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.240-248.2006
更新日期:2006-01-01 00:00:00
abstract::After thermal denaturation, an in vivo-labeled RNA was found in a temperature-sensitive initiation mutant of Bacillus subtilis (dna-37) associated with high-molecular-weight DNA. This RNA could be clearly distinguished from other RNA species by different techniques of separation, such as Sepharose 2B filtration, chrom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.79-91.1982
更新日期:1982-01-01 00:00:00