Abstract:
:Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative contributions of the autotrophic and heterotrophic systems to the growth of the organism. Wildtype H. eutropha grown under simultaneous conditions exhibited a dicyclic growth pattern, the first cycle representing either heterotrophic or simultaneous growth and the second cycle representing autotrophic growth. The duration of the changeover period was either very short with no plateau or long with a plateau up to 8 hr, depending upon the organic substrate. The growth rate under simultaneous conditions with some organic substrates was faster than either the autotrophic or heterotrophic rate, but was not the sum of the two rates. The data suggest that, in the presence of both organic and inorganic substrates, heterotrophic metabolism functions normally but autotrophic metabolism is partially repressed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
DeCicco BT,Stukus PEdoi
10.1128/JB.95.4.1469-1475.1968subject
Has Abstractpub_date
1968-04-01 00:00:00pages
1469-75issue
4eissn
0021-9193issn
1098-5530journal_volume
95pub_type
杂志文章abstract::The filamentous cyanobacterium Anabaena sp. strain PCC 7120 can fix N(2) under oxic conditions, and the activity of nitrogen fixation occurs exclusively in heterocysts, cells differentiated from vegetative cells in response to a limitation of a combined-nitrogen source in the growth medium. At the late stages of heter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00323-07
更新日期:2007-07-01 00:00:00
abstract::An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3482-3487.1997
更新日期:1997-06-01 00:00:00
abstract::The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01253-09
更新日期:2010-02-01 00:00:00
abstract::Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.546-558.1968
更新日期:1968-02-01 00:00:00
abstract::Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.1065-1067.1979
更新日期:1979-09-01 00:00:00
abstract::An in vitro constructed plasmid, pVH15, consisting of the entire genome of the plasmid ColE1, the tryptophan operon of Escherichia coli, and regions of the bacteriophage PHI80pt190, spontaneously gave rise in E. coli to a mini-ColE1 plasmid consisting of approximately one-half of the ColE1 genome and a small segment o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.447-453.1976
更新日期:1976-04-01 00:00:00
abstract::A specific gamma-aminobutyrate (GABA) transport system in Escherichia coli K-12 cells with a K(m) of 12 muM and a V(max) of 278 nmol/ml of intracellular water per min is described. Membrane vesicles contained d-lactate-dependent activity of the system. Mutants defective in GABA transport were isolated; they lost the a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.295-299.1978
更新日期:1978-08-01 00:00:00
abstract::The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1431-1436.1967
更新日期:1967-11-01 00:00:00
abstract::The importance of proton movements was assessed in the moderate halophile Vibrio costicola. When anaerobic cells in acidic buffer (pH 6.5) were given an O2 pulse, protons were extruded regardless of the presence of Na+. At pH 8.5, however, V. costicola produced an acidic response to an O2 pulse in the absence of Na+ a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.681-686.1985
更新日期:1985-02-01 00:00:00
abstract::The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01069-13
更新日期:2014-02-01 00:00:00
abstract::In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00674-10
更新日期:2010-11-01 00:00:00
abstract::Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00932-07
更新日期:2007-10-01 00:00:00
abstract::In Caulobacter crescentus biogenesis of the flagellar organelle occurs during one stage of its complex life cycle. Thus in synchronous cultures it is possible to assay the sequential synthesis and assembly of the flagellum and hook in vivo with a combination of biochemical and radioimmunological techniques. The period...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.1062-1069.1978
更新日期:1978-09-01 00:00:00
abstract::Proteus mirabilis, an agent of urinary tract infection, expresses at least four fimbrial types. Among these are the MR/P (mannose-resistant/Proteus-like) fimbriae. MrpA, the structural subunit, is optimally expressed at 37 degrees C in Luria broth cultured statically for 48 h by each of seven strains examined. Genes e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.11.3412-3419.1994
更新日期:1994-06-01 00:00:00
abstract::The anaerobic spore former Clostridioides difficile causes significant diarrheal disease in humans and other mammals. Infection begins with the ingestion of dormant spores, which subsequently germinate within the host gastrointestinal tract. There, the vegetative cells proliferate and secrete two exotoxins, TcdA and T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00586-19
更新日期:2020-01-02 00:00:00
abstract::Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00323-12
更新日期:2012-05-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.4.1455-1464.2005
更新日期:2005-02-01 00:00:00
abstract::Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.895-900.1963
更新日期:1963-04-01 00:00:00
abstract::In this study, we show that Salmonella produces an O-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. Structural analysis of purified Salmonella extracellular polysaccharides yielded predominantly a repeating oligosaccharide unit similar to that of Salmonella enterica serova...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00809-06
更新日期:2006-11-01 00:00:00
abstract::A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1651-1655.1987
更新日期:1987-04-01 00:00:00
abstract::The endoplasmic reticulum from Neurospora crassa was identified by monitoring the activity of the putative enzyme marker phosphatidylcholine glyceride transferase. After differential centrifugation of a cell homogenate, phosphatidylcholine glyceride transferase activity initially copurified with plasma membrane H+-ATP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.362-368.1983
更新日期:1983-10-01 00:00:00
abstract::The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3375-3380.1998
更新日期:1998-07-01 00:00:00
abstract::Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1090-1100.1974
更新日期:1974-06-01 00:00:00
abstract::An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.966-972.1993
更新日期:1993-02-01 00:00:00
abstract::Transferable plasmids play an important role in the dissemination of clindamycin-erythromycin resistance in Bacteroides fragilis. We previously described the isolation and properties of pBFTM10, a 14.9-kb ClnR transfer factor from B. fragilis TMP10. We also reported the isolation of a transfer-deficient deletion deriv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7471-7480.1991
更新日期:1991-12-01 00:00:00
abstract::For cells of the yeast Saccharomyces cerevisiae, the size at initiation of budding is proportional to growth rate for rates from 0.33 to 0.23 h-1. At growth rates lower than 0.23 h-1, cells displayed a minimum cell size at bud initiation independent of growth rate. Regardless of growth rate, cells displayed an increas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.1-5.1979
更新日期:1979-01-01 00:00:00
abstract::The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of beta-mercaptoethanol or incubated with thiol-specific reagents. The enzyme was protected from inactivation in the presence ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.18.4799-4803.1998
更新日期:1998-09-01 00:00:00
abstract::We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts transla...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.757-763.1973
更新日期:1973-11-01 00:00:00
abstract::Two active lysine transport systems were detected in Saccharomycopsis lipolytica. No excretion of lysine out of the cells could be obtained, even by chasing with L-lysine or by poisoning with sodium azide. The kinetic properties of one of the permeases, the high-affinity lysine permease, were studied in detail. Its Km...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.492-498.1978
更新日期:1978-02-01 00:00:00
abstract::We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 protein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01472-12
更新日期:2012-12-01 00:00:00