Transduction in Bacillus subtilis.

abstract：:The yeast Saccharomyces cerevisiae has two separate genes (APT1 and APT2) that encode two potentially different forms of adenine phosphoribosyltransferase (APRT). However, genetic analysis indicated that only APT1 could code for a complementing activity. Cloning and expression of both the APT1 and APT2 genes in Escher...

journal_title：Journal of bacteriology

authors： Alfonzo JD,Crother TR,Guetsova ML,Daignan-Fornier B,Taylor MW

更新日期：1999-01-01 00:00:00

abstract：:Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

journal_title：Journal of bacteriology

authors： Short KA,Blakemore RP

更新日期：1986-08-01 00:00:00

abstract：:A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synth...

journal_title：Journal of bacteriology

authors： Guardiola J,De Felice M,Iaccarino M

更新日期：1974-10-01 00:00:00

abstract：:The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

journal_title：Journal of bacteriology

authors： Barnett TC,Scott JR

更新日期：2002-04-01 00:00:00

abstract：:The physiological changes in an F1-ATPase-defective mutant of Escherichia coli W1485 growing in a glucose-limited chemostat included a decreased growth yield (60%) and increased specific rates of both glucose consumption (168%) and respiration (171%). Flux analysis revealed that the mutant showed approximately twice a...

journal_title：Journal of bacteriology

authors： Noda S,Takezawa Y,Mizutani T,Asakura T,Nishiumi E,Onoe K,Wada M,Tomita F,Matsushita K,Yokota A

更新日期：2006-10-01 00:00:00

abstract：:The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...

journal_title：Journal of bacteriology

authors： Arrieta JG,Sotolongo M,Menéndez C,Alfonso D,Trujillo LE,Soto M,Ramírez R,Hernández L

更新日期：2004-08-01 00:00:00

abstract：:A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complement...

journal_title：Journal of bacteriology

authors： Cameron B,Guilhot C,Blanche F,Cauchois L,Rouyez MC,Rigault S,Levy-Schil S,Crouzet J

更新日期：1991-10-01 00:00:00

abstract：:Biochemical and immunological experiments have suggested that the Escherichia coli enzyme p-aminobenzoate synthetase and anthranilate synthetase are structurally related. Both enzymes are composed of two nonidentical subunits. Anthranilate synthetase is composed of proteins encoded by the genes trp(G)D and trpE, where...

journal_title：Journal of bacteriology

authors： Goncharoff P,Nichols BP

更新日期：1984-07-01 00:00:00

abstract：:Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...

journal_title：Journal of bacteriology

authors： Pérez-Pantoja D,Ledger T,Pieper DH,González B

更新日期：2003-03-01 00:00:00

abstract：:Genetic studies have identified a specificity domain for prohead binding in the C-terminal 32 amino acids of gpA, the large subunit of bacteriophage lambda terminase (S. Frackman, D. A. Siegele, and M. Feiss, J. Mol. Biol. 180:283-300, 1984). In the present work, an amber mutation, Aam42, in the fifth-to-last codon of...

journal_title：Journal of bacteriology

authors： Sippy J,Feiss M

更新日期：1992-02-01 00:00:00

abstract：:Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...

journal_title：Journal of bacteriology

authors： Braunstein M,Griffin TJ IV,Kriakov JI,Friedman ST,Grindley ND,Jacobs WR Jr

更新日期：2000-05-01 00:00:00

abstract：:The DNA-dependent RNA polymerase (EC 2.7.7.6) from Clostridium acetobutylicum DSM 1731 has been purified to homogeneity and characterized. The purified enzyme was composed of four subunits and had a molecular mass of 370,000 Da. Western immunoblot analysis with polyclonal antibodies against the sigma 70 subunit of Esc...

journal_title：Journal of bacteriology

authors： Pich A,Bahl H

更新日期：1991-03-01 00:00:00

abstract：:A pattern of allosteric control for aromatic biosynthesis in cyanobacteria relies upon early-pathway regulation as the major control point for the entire branched pathway. In Synechococcus sp. strain PCC6301 (Anacystis nidulans), two enzymes which form precursors for L-phenylalanine biosynthesis are subject to control...

journal_title：Journal of bacteriology

authors： Hall GC,Flick MB,Jensen RA

更新日期：1983-01-01 00:00:00

abstract：:Resistance to the antifolates methasquin and amethopterin has been studied in new strains of Streptococcus faecium var. durans. Two methasquin-resistant strains (SF/MQ, SF/MQ(T)) and an amethopterin-resistant strain (SF/AM) were selected independently from the wild-type S. faecium var. durans (SF/O). SF/MQ(T) is a thy...

journal_title：Journal of bacteriology

authors： Rader JI,Hutchison DJ

更新日期：1972-04-01 00:00:00

abstract：:The number and disposition of flagella harbored by eubacteria are regulated by a specific trait successfully maintained over generations. The genes governing the number of flagella in Bacillus subtilis have never been identified, although the ifm locus has long been recognized to influence the motility phenotype of th...

journal_title：Journal of bacteriology

authors： Calvio C,Celandroni F,Ghelardi E,Amati G,Salvetti S,Ceciliani F,Galizzi A,Senesi S

更新日期：2005-08-01 00:00:00

abstract：:Mycoplasma showing at least two colony types were isolated from the nares and oropharynx of New Zealand white rabbits. Two strains were purified by single-colony passages and characterized. Morphology by phase-contrast and electron microscopy was typical of Mycoplasmataceae. Both grew anaerobically as well as aerobica...

journal_title：Journal of bacteriology

authors： Deeb BJ,Kenny GE

更新日期：1967-04-01 00:00:00

abstract：:In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

journal_title：Journal of bacteriology

authors： Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

更新日期：2017-05-09 00:00:00

abstract：:The Escherichia coli outer membrane protein Tsx functions as a nucleoside-specific channel and serves as the receptor for colicin K and a number of T-even-type bacteriophages, including phage T6. To identify those segments of the Tsx protein that are important for its phage receptor function, we devised a selection an...

journal_title：Journal of bacteriology

authors： Schneider H,Fsihi H,Kottwitz B,Mygind B,Bremer E

更新日期：1993-05-01 00:00:00

abstract：:The Salmonella typhimurium aroF gene, encoding the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, was localized to a chromosomal PstI fragment by Southern blotting with an Escherichia coli aroF probe. This fragment was cloned by screening a plasmid library for complementation of an E. ...

journal_title：Journal of bacteriology

authors： Muday GK,Herrmann KM

更新日期：1990-05-01 00:00:00

abstract：:The buoyant density in rapidly growing Streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in Escherichia coli. Buoyant density in S. faecium was measured by using Percoll (Pharmacia Fine Chemicals, Piscataway, N.J.) density gradients. We found that the mean and coefficient of varia...

journal_title：Journal of bacteriology

authors： Glaser D,Higgins M

更新日期：1989-02-01 00:00:00

abstract：:The complete sequences of the RhsB and RhsC elements of Escherichia coli K-12 have been determined. These sequence data reveal a new repeated sequence, called H-rpt (Hinc repeat), which is distinct from the Rhs core repetition that is found in all five Rhs elements. H-rpt is found in RhsB, RhsC, and RhsE. Characteriza...

journal_title：Journal of bacteriology

authors： Zhao S,Sandt CH,Feulner G,Vlazny DA,Gray JA,Hill CW

更新日期：1993-05-01 00:00:00

abstract：:Different preparations of the methylreductase were tested in a simplified methylcoenzyme M methylreductase assay with artificial electron donors under a nitrogen atmosphere. ATP and Mg2+ stimulated the reaction. Tris(2,2'-bipyridine)ruthenium (II), chromous chloride, chromous acetate, titanium III citrate, 2,8-diamino...

journal_title：Journal of bacteriology

authors： Hartzell PL,Escalante-Semerena JC,Bobik TA,Wolfe RS

更新日期：1988-06-01 00:00:00

abstract：:Cyanobacteria are monophyletic organisms that perform oxygenic photosynthesis. While they exhibit great diversity, they have a common set of genes. However, the essentiality of them for viability has hampered the elucidation of their functions. One example of these genes is cyabrB1 (also known as calA in Anabaena sp. ...

journal_title：Journal of bacteriology

authors： Higo A,Nishiyama E,Nakamura K,Hihara Y,Ehira S

更新日期：2019-08-08 00:00:00

abstract：:Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...

journal_title：Journal of bacteriology

authors： Langridge P,Morita RY

更新日期：1966-08-01 00:00:00

abstract：:Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

journal_title：Journal of bacteriology

authors： Brenner DJ,Fanning GR,Steigerwalt AG

更新日期：1972-04-01 00:00:00

abstract：:ParB is one of two P1-encoded proteins that are required for active partition of the P1 prophage in Escherichia coli. To probe the native domain structure of ParB, we performed limited proteolytic digestions of full-length ParB, as well as of several N-terminal and C-terminal deletion fragments of ParB. The C-terminal...

journal_title：Journal of bacteriology

authors： Surtees JA,Funnell BE

更新日期：1999-10-01 00:00:00

abstract：:The Mycobacterium tuberculosis gene Rv2747 encodes a novel 19-kDa ArgA that catalyzes the initial step in L-arginine biosynthesis, namely the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. Initial velocity studies reveal that Rv2747 proceeds through a sequential kinetic mechanism, with K(m) values of 280 mM ...

journal_title：Journal of bacteriology

authors： Errey JC,Blanchard JS

更新日期：2005-05-01 00:00:00

abstract：:Bacillus subtilis OhrR is the prototype for the one-Cys family of organic peroxide-sensing regulatory proteins. Mutational analyses indicate that the high sensitivity of the active site cysteine (C15) to peroxidation requires three Tyr residues. Y29 and Y40 from the opposing subunit of the functional dimer hydrogen bo...

journal_title：Journal of bacteriology

authors： Soonsanga S,Fuangthong M,Helmann JD

更新日期：2007-10-01 00:00:00

abstract：:Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...

journal_title：Journal of bacteriology

authors： Aronson AI,Bell C,Fulroth B

更新日期：2005-05-01 00:00:00

abstract：:Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

journal_title：Journal of bacteriology

authors： Schrempf H,Bujard H,Hopwood DA,Goebel W

更新日期：1975-02-01 00:00:00