Abstract:
:ParB is one of two P1-encoded proteins that are required for active partition of the P1 prophage in Escherichia coli. To probe the native domain structure of ParB, we performed limited proteolytic digestions of full-length ParB, as well as of several N-terminal and C-terminal deletion fragments of ParB. The C-terminal 140 amino acids of ParB form a very trypsin-resistant domain. In contrast, the N terminus is more susceptible to proteolysis, suggesting that it forms a less stably folded domain or domains. Because native ParB is a dimer in solution, we analyzed the ability of ParB fragments to dimerize, using both the yeast two-hybrid system and in vitro chemical cross-linking of purified proteins. These studies revealed that the C-terminal 59 amino acids of ParB, a region within the protease-resistant domain, are sufficient for dimerization. Cross-linking and yeast two-hybrid experiments also revealed the presence of a second self-association domain within the N-terminal half of ParB. The cross-linking data also suggest that the C terminus is inhibitory to multimerization through the N-terminal domain in vitro. We propose that the two multimerization domains play distinct roles in partition complex formation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Surtees JA,Funnell BEdoi
10.1128/JB.181.19.5898-5908.1999keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
5898-908issue
19eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01471-13
更新日期:2014-04-01 00:00:00
abstract::A mutation in Escherichia coli leads to the loss of ribosomal protein L24, severely impaired growth, and a temperature-sensitive phenotype. The mutation was shown to be in rplX, the gene for protein L24, and was due to the alteration of an AAA codon to a TAA stop codon at position 61 in rplX that resulted in a 20-amin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.890-894.1985
更新日期:1985-09-01 00:00:00
abstract::A class II restriction endonuclease which recognizes the same nucleotide sequence as EndoR.HaeIII has been found in four of seven isolates of Neisseria gonorrhoeae tested. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.1.270-273.1978
更新日期:1978-07-01 00:00:00
abstract::We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.789-794.1984
更新日期:1984-03-01 00:00:00
abstract::We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localiza...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00395-08
更新日期:2008-07-01 00:00:00
abstract::The addition of 5',5',5'-trifluoroleucine (fluoroleucine) to leucine auxotrophs of Salmonella typhimurium permitted protein but not ribonucleic acid (RNA) synthesis to continue after leucine depletion. The uncoupling of the formation of these macromolecules by fluoroleucine was apparent if RNA and protein synthesis wa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.107-112.1969
更新日期:1969-07-01 00:00:00
abstract::A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.1.278-286.1978
更新日期:1978-04-01 00:00:00
abstract::The extent of change in cytoplasmic proteins which accompanies yeast-to-mycelium morphogenesis of Candida albicans was analyzed by two-dimensional gel electrophoresis. Pure cultures of yeasts and true hyphae (i.e., without concomitant production of pseudohyphae) were grown in a synthetic low-sulfate medium. The two st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.258-273.1980
更新日期:1980-10-01 00:00:00
abstract::Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.788-795.1981
更新日期:1981-02-01 00:00:00
abstract::Biochemical and immunological experiments have suggested that the Escherichia coli enzyme p-aminobenzoate synthetase and anthranilate synthetase are structurally related. Both enzymes are composed of two nonidentical subunits. Anthranilate synthetase is composed of proteins encoded by the genes trp(G)D and trpE, where...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.57-62.1984
更新日期:1984-07-01 00:00:00
abstract::Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the heterodimeric, membrane-bound hydrogenase (MBH) are located adjacent to a series of eight accessory genes (hoxZ, hoxM, h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4522-4529.1996
更新日期:1996-08-01 00:00:00
abstract::Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.270-274.1980
更新日期:1980-01-01 00:00:00
abstract::We have presented experimental evidence to support the model that gliding motility of Myxococcus xanthus is driven by surface tension. (i) Motility is inhibited by the addition of sufficient exogenous, nontoxic surfactants to swamp out the cells' own surfactant gradient. (ii) M. xanthus does not move polystyrene latex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1367-1371.1983
更新日期:1983-09-01 00:00:00
abstract::Rhizobium sp. strain NGR234 produces a large family of lipochitooligosaccharide Nod factors carrying specific substituents. Among them are 3-O- (or 4-O-) and 6-O-carbamoyl groups, an N-methyl group, and a 2-O-methylfucose residue which may bear either 3-O-sulfate or 4-O-acetyl substitutions. Investigations on the gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.16.5087-5093.1997
更新日期:1997-08-01 00:00:00
abstract::The marine bacterium and human pathogen Vibrio parahaemolyticus rapidly colonizes surfaces by using swarming motility and forming robust biofilms. Entering one of the two colonization programs, swarming motility or sessility, involves differential regulation of many genes, resulting in a dramatic shift in physiology a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00723-19
更新日期:2020-02-25 00:00:00
abstract::Hg2+-resistant mutants were isolated from Saccharomyces cerevisiae. Although they were very much like the parental strains in terms of colony-forming ability, they grew faster than the parental strains in the presence of sublethal doses of Hg2+. The Hg2+-resistant mutations were dominant. They were centromere linked a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5877-5882.1988
更新日期:1988-12-01 00:00:00
abstract::To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.7111-7117.1997
更新日期:1997-11-01 00:00:00
abstract::A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.167-177.1973
更新日期:1973-01-01 00:00:00
abstract::Rod-shaped bacteria grow by a repetitive cycle of elongation followed by division, and the mechanisms responsible for these two processes have been studied for decades. However, little is known about what happens during the transition between the two activities. At least one event occurs after elongation ends and befo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00859-12
更新日期:2012-10-01 00:00:00
abstract::A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1186-1189.1974
更新日期:1974-06-01 00:00:00
abstract::We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4665-4684.2004
更新日期:2004-07-01 00:00:00
abstract::Streptomyces sp. strain HK803 produces six analogues of phoslactomycin (Plm A through Plm F). With the exception of Plm B, these analogues contain a C-18 hydroxyl substituent esterified with a range of short-alkyl-chain carboxylic acids. Deletion of the plmS(2) open reading frame (ORF), showing high sequence similarit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.23.7970-7976.2005
更新日期:2005-12-01 00:00:00
abstract::In Escherichia coli, the GlpT transporter, a member of the major facilitator superfamily, moves external glycerol 3-phosphate (G3P) into the cytoplasm in exchange for cytoplasmic phosphate. Study of intact cells showed that both GlpT and HisGlpT, a variant with an N-terminal six-histidine tag, are inhibited (50% inhib...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3863-3870.2003
更新日期:2003-07-01 00:00:00
abstract::Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-defi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02277-12
更新日期:2013-04-01 00:00:00
abstract::The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00995-13
更新日期:2014-01-01 00:00:00
abstract::Hyphae of Aspergillus nidulans continued to synthesize all the major polysaccharide components of the cell wall when cycloheximide was added to cultures. In the presence of cycloheximide, hyphae did not elongate, but electron microscopy showed that the walls became thicker around the cell. The conclusion that cyclohex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.819-823.1973
更新日期:1973-05-01 00:00:00
abstract::Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.1.206-211.1995
更新日期:1995-01-01 00:00:00
abstract::Bacillus thuringiensis var. thuringiensis Berliner grown at 14 C on a chemically defined liquid medium slowly produced spores and parasporal inclusions. Sporulation ultimately attained 50 to 100% of that of cultures grown at 30 C. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.445-448.1971
更新日期:1971-01-01 00:00:00
abstract::The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4637-4639.2000
更新日期:2000-08-01 00:00:00
abstract::Members of all four families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Members of Methanobacteriaceae (family I) were characterized by very low polyamine concentra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1315-1322.1983
更新日期:1983-06-01 00:00:00