Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis.

abstract：:The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...

journal_title：Journal of bacteriology

authors： Chen MW,Kotaka M,Vonrhein C,Bricogne G,Rao F,Chuah ML,Svergun D,Schneider G,Liang ZX,Lescar J

更新日期：2012-09-01 00:00:00

abstract：:The filamentous cyanobacterium Microcoleus vaginatusis found in arid land soils worldwide. The genome of M. vaginatus strain FGP-2 allows exploration of genes involved in photosynthesis, desiccation tolerance, alkane production, and other features contributing to this organism's ability to function as a major componen...

journal_title：Journal of bacteriology

authors： Starkenburg SR,Reitenga KG,Freitas T,Johnson S,Chain PS,Garcia-Pichel F,Kuske CR

更新日期：2011-09-01 00:00:00

abstract：:The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...

journal_title：Journal of bacteriology

authors： Kargalioglu Y,Imlay JA

更新日期：1994-12-01 00:00:00

abstract：:Shigella flexneri is a gram-negative bacterium that causes diarrhea and dysentery by invasion and spread through the colonic epithelium. Bacteria spread by assembling actin and other cytoskeletal proteins of the host into "actin tails" at the bacterial pole; actin tail assembly provides the force required to move bact...

journal_title：Journal of bacteriology

authors： Charles M,Magdalena J,Theriot JA,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

journal_title：Journal of bacteriology

authors： Shioi JI,Matsuura S,Imae Y

更新日期：1980-12-01 00:00:00

abstract：:The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...

journal_title：Journal of bacteriology

authors： Dhiman A,Schleif R

更新日期：2000-09-01 00:00:00

abstract：:A segment of Escherichia coli DNA that contained a discontinuity of homology with Salmonella typhimurium DNA was isolated. The segment, 1,430 base pairs long, was derived from one end of the lac "loop," a region of about 12 kilobase pairs of E. coli DNA, including the lac operon which has no detectable homology with S...

journal_title：Journal of bacteriology

authors： Buvinger WE,Lampel KA,Bojanowski RJ,Riley M

更新日期：1984-08-01 00:00:00

abstract：:The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, ...

journal_title：Journal of bacteriology

authors： Ullmann WW,Cameron JA

更新日期：1969-05-01 00:00:00

abstract：:Several authors have suggested that the SOS-associated (sfiA-dependent) system of division inhibition, normally induced by perturbations of DNA replication, also regulates steady-state (unperturbed) cell division. The present work shows that mean cell mass is identical in sfiA+ and sfiA mutant cultures during steady-s...

journal_title：Journal of bacteriology

authors： Huisman O,Jacques M,D'ari R,Caro L

更新日期：1983-02-01 00:00:00

abstract：:Sguros, Peter L. (University of Miami, Miami, Fla.), and Jacqueline Simms. Role of marine fungi in the biochemistry of the oceans. IV. Growth responses to seawater inorganic macroconstituents. J. Bacteriol. 88:346-355. 1964.-The filamentous ascomycete Halosphaeria mediosetigera and the deuteromycete Culcitalna achrasp...

journal_title：Journal of bacteriology

authors： SGUROS PL,SIMMS J

更新日期：1964-08-01 00:00:00

abstract：:The aquatic bacterium Caulobacter crescentus attaches to solid surfaces through an adhesive holdfast located at the tip of its polar stalk, a thin cylindrical extension of the cell membrane. In this paper, the elastic properties of the C. crescentus stalk and holdfast assembly were studied by using video light microsc...

journal_title：Journal of bacteriology

authors： Li G,Smith CS,Brun YV,Tang JX

更新日期：2005-01-01 00:00:00

abstract：:The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

journal_title：Journal of bacteriology

authors： Duncan CL,Sugiyama H,Strong DH

更新日期：1968-05-01 00:00:00

abstract：:We have previously shown that the TolA protein is required for the correct surface expression of the Escherichia coli O7 antigen lipopolysaccharide (LPS). In this work, delta tolA and delta pal mutants of E. coli K-12 W3110 were transformed with pMF19 (encoding a rhamnosyltransferase that reconstitutes the expression ...

journal_title：Journal of bacteriology

authors： Vinés ED,Marolda CL,Balachandran A,Valvano MA

更新日期：2005-05-01 00:00:00

abstract：:The structural genes of ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) from Salmonella typhimurium LT2 were cloned on a 5.8-kilobase-pair insert in the SalI site of pBR322. A single strand specific radioactive probe containing the N terminus of the Escherichia coli K-12 glgC gene in M13mp8 was used t...

journal_title：Journal of bacteriology

authors： Leung PS,Preiss J

更新日期：1987-09-01 00:00:00

abstract：:Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

journal_title：Journal of bacteriology

authors： HARPER MK,CURRAN HR,PALLANSCH MJ

更新日期：1964-11-01 00:00:00

abstract：:The prfA gene of Listeria monocytogenes was recently reported to be required for expression of hly, which encodes a pore-forming hemolysin essential for pathogenicity (M. Leimeister-Wachter, C. Haffner, E. Domann, W. Goebel, and T. Chakraborty, Proc. Natl. Acad. Sci. USA 87:8336-8340, 1990). We demonstrate here that a...

journal_title：Journal of bacteriology

authors： Freitag NE,Youngman P,Portnoy DA

更新日期：1992-02-01 00:00:00

abstract：:Bacteria possess multiple mechanisms to survive exposure to various chemical stresses and antimicrobial compounds. In the enteric bacterium Escherichia coli, three homologous transcription factors-MarA, SoxS, and Rob-play a central role in coordinating this response. Three separate systems are known to regulate the ex...

journal_title：Journal of bacteriology

authors： Chubiz LM,Glekas GD,Rao CV

更新日期：2012-09-01 00:00:00

abstract：:Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...

journal_title：Journal of bacteriology

authors： Kingan SL,Ensign JC

更新日期：1968-09-01 00:00:00

abstract：:Hertman, I. (Israel Institute for Biological Research, Ness Ziona, Israel). Bacteriophage common to Pasteurella pestis and Escherichia coli. J. Bacteriol. 88:1002-1005. 1964.-Phage Y of Pasteurella pestis and phage T(3) of Escherichia coli are serologically related and have common bacterial hosts. Anti-Y serum neutral...

journal_title：Journal of bacteriology

authors： HERTMAN I

更新日期：1964-10-01 00:00:00

abstract：:A 200-megadalton plasmid was mobilized from Rhizobium japonicum USDA 191 to other Rhizobium strains either that cannot nodulate soybeans or that form Fix- nodules on certain cultivars. The symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nit...

journal_title：Journal of bacteriology

authors： Appelbaum ER,McLoughlin TJ,O'Connell M,Chartrain N

更新日期：1985-07-01 00:00:00

abstract：:A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the eff...

journal_title：Journal of bacteriology

authors： Katsumata R,Ozaki A,Oka T,Furuya A

更新日期：1984-07-01 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:The DNA of bacteriophage Mu, extracted from induced lysates, is partially resistant to digestion by the endonuclease BalI. This modification of DNA is controlled by the Mu modification function (mom), which acts in conjunction with the dam (DNA-adenine methylation) function of Escherichia coli. Since the BalI recognit...

journal_title：Journal of bacteriology

authors： Khatoon H,Bukhari AI

更新日期：1978-10-01 00:00:00

abstract：:In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

journal_title：Journal of bacteriology

authors： Myers CR,Myers JM

更新日期：1992-06-01 00:00:00

abstract：:Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...

journal_title：Journal of bacteriology

authors： Schrader JA,Holmes DS

更新日期：1988-09-01 00:00:00

abstract：:Phospholipid synthesis has been reported to be subject to stringent control in Escherichia coli. We present evidence that demonstrates a strict correlation between guanosine tetraphosphate accumulation and inhibition of phospholipid synthesis. In vivo experiments designed to examine the pattern of phospholipid labelin...

journal_title：Journal of bacteriology

authors： Merlie JP,Pizer LI

更新日期：1973-10-01 00:00:00

abstract：:The kinetics of peptidoglycan degradation were examined under different conditions of autolysis of Escherichia coli. With cephaloridine- or moenomycin-induced autolysis, degradation did not exceed 25 to 35%, whereas in EDTA-induced autolysis it rapidly reached 65 to 70%. When nonautolyzing cells were fixed overnight w...

journal_title：Journal of bacteriology

authors： Leduc M,Frehel C,van Heijenoort J

更新日期：1985-02-01 00:00:00

abstract：:Low-copy-number plasmids, such as P1 prophage and the fertility factor F, require a plasmid-encoded replication protein and several host products for replication. Stable maintenance also depends on active partitioning of plasmids into daughter cells. Mini-P1 par+ and par plasmids were found to be destabilized by mutat...

journal_title：Journal of bacteriology

authors： Tilly K,Yarmolinsky M

更新日期：1989-11-01 00:00:00

abstract：:Growth of Schizosaccharomyces pombe in batch cultures in a commercial defined medium was found to be normal until the last generation before the stationary phase. Approximately one-half the progeny of the last division lysed. Lysis occurred at breaks in the cell wall at cell-plates whose fission was eccentric. Normal ...

journal_title：Journal of bacteriology

authors： Johnson BF

更新日期：1967-07-01 00:00:00

abstract：:We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...

journal_title：Journal of bacteriology

authors： Davis LC,Wang YL

更新日期：1980-03-01 00:00:00