Abstract:
:Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type population. Several morphological and physiological characteristics of a class of T. ferrooxidans variants, termed LSC for large spreading colony, are described. LSC variants lack the ability to oxidize iron but retain the capacity to utilize thiosulfate or tetrathionate as energy sources. An LSC colony spreads on the surface of solid 100:10 medium as a monolayer of cells in a fashion resembling that of certain swarming or gliding bacteria. The LSC variant reverts to a parental wild type at frequencies that vary in different independently arising isolates. The identity of the LSC variant as a derivative of the parental wild-type T. ferrooxidans was established by Southern blot hybridization.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schrader JA,Holmes DSdoi
10.1128/jb.170.9.3915-3923.1988subject
Has Abstractpub_date
1988-09-01 00:00:00pages
3915-23issue
9eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::The cell walls of selected bacteria were studied by X-ray diffraction analysis to determine and characterize crystalline components. The walls were isolated by mechanical disruption and purified by enzymatic and washing procedures. The X-ray diffraction lines which appeared from the gram-positive cell walls were shown...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.122-124.1967
更新日期:1967-01-01 00:00:00
abstract::Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilizatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01219-10
更新日期:2011-03-01 00:00:00
abstract::The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5539-5545.1991
更新日期:1991-09-01 00:00:00
abstract::AdnA is a transcription factor in Pseudomonas fluorescens that affects flagellar synthesis, biofilm formation, and sand adhesion. To identify the AdnA regulon, we used a promoterless Tn5-lacZ element to study the phenotypes of insertion mutants in the presence and absence of AdnA. Of 12,000 insertions, we identified s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.453-460.2003
更新日期:2003-01-01 00:00:00
abstract::Gordon, M. A. (New York State Department of Health, Albany). The genus Dermatophilus. J. Bacteriol. 88:509-522. 1964.-Seventeen strains of Dermatophilus originating in skin lesions of cattle, sheep, horses, deer, and man were compared as to conditions for growth, colonial characteristics under varying conditions, micr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.2.509-522.1964
更新日期:1964-08-01 00:00:00
abstract::PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.1.119-128.1972
更新日期:1972-07-01 00:00:00
abstract::It has been previously established that Thiobacillus neapolitanus fixes CO2 by using a form I ribulose bisphosphate carboxylase/oxygenase (RuBisCO), that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL and cbbS, are part of a putative carboxysome operon. In the present stud...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.16.4133-4139.1998
更新日期:1998-08-01 00:00:00
abstract::Nitrogen fixation occurs in two domains, Archaea and Bacteria. We have characterized a nif (nitrogen fixation) gene cluster in the methanogenic archaeon Methanococcus maripaludis. Sequence analysis revealed eight genes, six with sequence similarity to known nif genes and two with sequence similarity to glnB. The gene ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.6.1504-1511.1998
更新日期:1998-03-01 00:00:00
abstract::An expressed sequence tag homologous to cheA was previously isolated by random sequencing of Thermotoga maritima cDNA clones (C. W. Kim, P. Markiewicz, J. J. Lee, C. F. Schierle, and J. H. Miller, J. Mol. Biol. 231: 960-981, 1993). Oligonucleotides complementary to this sequence tag were synthesized and used to identi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.2.484-489.1996
更新日期:1996-01-01 00:00:00
abstract::The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01290-07
更新日期:2008-02-01 00:00:00
abstract::Myxobacterial hemagglutinin (MBHA) is a major developmentally induced protein that accumulates during the period of cellular aggregation in the bacterium Myxococcus xanthus. It has been shown that this lectin is targeted to the cell surface and periplasmic space of developmental cells, suggesting that it may play a ro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3801-3808.1987
更新日期:1987-08-01 00:00:00
abstract::Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::In some media, Bacillus subtilis can maintain a prolonged stationary growth phase; however, in other media, nutrient depletion triggers a complex differentiation that culminates in production of a dormant endospore. This differentiation requires the expression of many genes. We found that during the stationary phase i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.709-715.1990
更新日期:1990-02-01 00:00:00
abstract::Methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MT1) is the first of two enzymes required for transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid in Methanosarcina barkeri. MT1 binds the methyl group of methanol to its corrinoid prosthetic group only when the central cobalt atom of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1278-1283.1993
更新日期:1993-03-01 00:00:00
abstract::If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.435-444.1975
更新日期:1975-10-01 00:00:00
abstract::The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.24.7653-7658.1994
更新日期:1994-12-01 00:00:00
abstract::Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00181-06
更新日期:2006-06-01 00:00:00
abstract::Burkholderia pseudomallei is the causative agent of melioidosis, an often fatal infection of humans and animals. The virulence of this pathogen is thought to depend on a number of secreted proteins, including the MprA metalloprotease. We observed that MprA is produced upon entry into the stationary phase, when the cel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.8.2288-2294.2004
更新日期:2004-04-01 00:00:00
abstract::Pigmentation mutants of Pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. Three classes of mutants that were incapable of pyocyanine production were identified. Pigmentation patterns that were found to cha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.846-852.1979
更新日期:1979-06-01 00:00:00
abstract::The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.22.7045-7054.1994
更新日期:1994-11-01 00:00:00
abstract::Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.536-540.1963
更新日期:1963-03-01 00:00:00
abstract::"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00137-06
更新日期:2006-06-01 00:00:00
abstract:UNLABELLED:The latent period of phage T4, normally ∼25 min, can be extended indefinitely if the infected cell is superinfected after 5 min. This phenomenon, designated lysis inhibition (LIN), was first described in the 1940s and is genetically defined by mutations in diverse T4 r genes. RI, the main effector of LIN, ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00294-16
更新日期:2016-08-25 00:00:00
abstract::Bacterial chemotaxis is based on modulation of the probability to switch the direction of flagellar rotation. Responses to many stimuli are transduced by a two-component system via reversible phosphorylation of CheY, a small cytoplasmic protein that directly interacts with the switch complex at the flagellar motor. We...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6882-6887.1996
更新日期:1996-12-01 00:00:00
abstract::Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2124-2127.1989
更新日期:1989-04-01 00:00:00
abstract::In Pseudomonas putida DOT-T1E multidrug efflux pumps of the resistance-nodulation-division family make a major contribution to solvent resistance. Two pumps have been identified: TtgABC, expressed constitutively, and TtgDEF, induced by aromatic hydrocarbons. A double mutant lacking both efflux pumps was able to surviv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.13.3967-3973.2001
更新日期:2001-07-01 00:00:00
abstract::Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.18.5573-5584.2003
更新日期:2003-09-01 00:00:00
abstract:UNLABELLED:The GlnPQ transporter from Lactococcus lactis has the remarkable feature of having two substrate-binding domains (SBDs) fused to the N terminus of the transmembrane domain (TMD), and thus four SBDs are present in the homodimeric complex. Although X-ray structures and ligand binding data are available for bot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00685-15
更新日期:2015-11-09 00:00:00
abstract::A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6732-6741.1991
更新日期:1991-11-01 00:00:00
abstract::The number of rRNA genes in Mycobacterium bovis BCG was examined by Southern hybridization of end-labeled 5S, 16S, and 23S rRNAs with BamHI, PstI, and SalI digests of M. bovis BCG DNA. Each RNA probe gave only one radioactive band with three kinds of DNA digest. These results suggest that M. bovis BCG chromosomes may ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.839-843.1987
更新日期:1987-02-01 00:00:00