Abstract:
:Methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MT1) is the first of two enzymes required for transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid in Methanosarcina barkeri. MT1 binds the methyl group of methanol to its corrinoid prosthetic group only when the central cobalt atom of the corrinoid is present in the highly reduced Co(I) state. However, upon manipulation of MT1 and even during catalysis, the enzyme becomes inactivated as the result of Co(I) oxidation. Reactivation requires H2, hydrogenase, and ATP. Ferredoxin stimulated the apparent reaction rate of methyl group transfer. Here we report that one more protein fraction was found essential for the overall reaction and, more specifically, for formation of the methylated MT1 intermediate. The more of the protein that was present, the shorter the delay of the start of methyl group transfer. The maximum velocity of methyl transfer was not substantially affected by these varying amounts of protein. This demonstrated that the protein was involved in the activation of MT1. Therefore, it was called methyltransferase activation protein.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Daas PJ,Gerrits KA,Keltjens JT,van der Drift C,Vogels GDdoi
10.1128/jb.175.5.1278-1283.1993subject
Has Abstractpub_date
1993-03-01 00:00:00pages
1278-83issue
5eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract::In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2975-2977.1996
更新日期:1996-05-01 00:00:00
abstract::Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase wa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1502-1508.1967
更新日期:1967-11-01 00:00:00
abstract::The mutation sup-3 in Bacillus subtilis suppresses ochre (TAA) mutations at each of three codons in the 5' end of the cat-86 coding sequence. The suppressor is shown to insert lysine at ochre codons. The efficiency of suppression by sup-3 is about 15%, as determined by changing a cat-86 Lys codon (codon 12) to an ochr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5322-5324.1989
更新日期:1989-10-01 00:00:00
abstract::Cnm is a surface-associated protein present in a subset of Streptococcus mutans strains that mediates binding to extracellular matrices, intracellular invasion, and virulence. Here, we showed that cnm transcription is controlled by the global regulators CovR and VicRKX. In silico analysis identified multiple putative ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00141-18
更新日期:2018-11-06 00:00:00
abstract::Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3053-3059.1989
更新日期:1989-06-01 00:00:00
abstract::Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.1117-1122.1978
更新日期:1978-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.3053-3057.1988
更新日期:1988-07-01 00:00:00
abstract::Hydrogenotrophic methanogenic Archaea are defined by an H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. While certain responses of these organisms to hydrogen availability have been characterized, responses to fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02141-12
更新日期:2013-04-01 00:00:00
abstract::With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1228-1233.1986
更新日期:1986-12-01 00:00:00
abstract::Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1621-1623.1975
更新日期:1975-12-01 00:00:00
abstract::Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.3.1246-1253.1970
更新日期:1970-12-01 00:00:00
abstract::We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2612-2619.1992
更新日期:1992-04-01 00:00:00
abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00373-15
更新日期:2016-01-11 00:00:00
abstract::The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.175-183.1984
更新日期:1984-10-01 00:00:00
abstract::Sporolactobacillus inulinus CASD is an efficient D-lactic acid producer with high optical purity. Here we report for the first time the draft genome sequence of S. inulinus (2,930,096 bp). The large number of annotated two-component system genes makes it possible to explore the mechanism of extraordinary lactate toler...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05934-11
更新日期:2011-10-01 00:00:00
abstract::Concanavalin A-reactive linker and anchor subunits of phycobilisomes from Anacystis nidulans R2 (H. C. Riethman, T. P. Mawhinney, and L. A. Sherman, FEBS Lett. 215:209-214, 1987) were purified electrophoretically and analyzed for carbohydrate composition and quantity. Different quantities of glucose and N-acetylgalact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2433-2440.1988
更新日期:1988-06-01 00:00:00
abstract::The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1934-1939.1988
更新日期:1988-04-01 00:00:00
abstract::By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.334-341.1974
更新日期:1974-05-01 00:00:00
abstract::Peripheral hyphae were separated from the remaining thallus of Rhizoctonia solani in exponential and stationary phases of growth. The QO(2) in whole cells of peripheral hyphae from young fungal colonies was on the average 2.6 times and the protein content 1.6 times greater than in peripheral hyphae from old fungal col...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.640-645.1970
更新日期:1970-11-01 00:00:00
abstract::Evidence from pulse-chase experiments indicates that the outer membrane matrix protein or porin of Escherichia coli B/r passes through a Sarkosyl-soluble membrane pool on the way to its eventual Sarkosyl-insoluble state in the outer membrane. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1538-1541.1980
更新日期:1980-09-01 00:00:00
abstract::d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.687-696.1973
更新日期:1973-02-01 00:00:00
abstract::Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.375-379.1965
更新日期:1965-08-01 00:00:00
abstract::Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tet...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1268-1278.1991
更新日期:1991-02-01 00:00:00
abstract::Members of Thermococcales harbor a number of genes encoding putative aminotransferase Class III enzymes. Here, we characterized the TK1211 protein from the hyperthermophilic archaeon Thermococcus kodakarensis The TK1211 gene was expressed in T. kodakarensis under the control of the strong, constitutive promoter of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00617-20
更新日期:2021-01-19 00:00:00
abstract::The asg mutants of Myxococcus xanthus are defective in production of extracellular A-signal, which serves as a cell density signal for fruiting-body development. The DNA sequence of asgB, one of the three asg genes, was determined. The deduced amino acid sequence of AsgB contains a DNA-binding helix-turn-helix motif n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.2013-2020.1994
更新日期:1994-04-01 00:00:00
abstract::Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.1158-1161.1981
更新日期:1981-06-01 00:00:00
abstract::In vivo and in vitro methylation, methanol production assays, and the use of specific antibodies raised against the sensory transducing protein Tar in Escherichia coli all failed to demonstrate the presence of methyl-accepting chemotaxis proteins (MCPs) in the photosynthetic bacterium Rhodobacter sphaeroides, although...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5808-5814.1987
更新日期:1987-12-01 00:00:00
abstract::Deletion mutagenesis analysis of a duplicated gene necessary for Haemophilus influenzae serotype b capsule expression showed that only one functional copy of this gene is required for capsule production and for virulence in infant rats. Mutant strains generated in this study differed from each other and from the paren...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.44-48.1986
更新日期:1986-07-01 00:00:00
abstract::Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.7.2219-2225.2001
更新日期:2001-04-01 00:00:00
abstract::An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.966-972.1993
更新日期:1993-02-01 00:00:00