Determination of Wolbachia genome size by pulsed-field gel electrophoresis.

Abstract:

:Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number of sites and were used for the determination of the genome sizes of wMelPop, wMel, and wMelCS (each 1.36 Mb), wRi (1.66 Mb), wBma (1.1 Mb), and wDim (0.95 Mb). The Wolbachia genomes studied were all much smaller than the genomes of free-living bacteria such as Escherichia coli (4.7 Mb), as is typical for obligate intracellular bacteria. There was considerable genome size variability among Wolbachia strains, especially between the more parasitic A group Wolbachia infections of insects and the mutualistic C and D group infections of nematodes. The studies described here found no evidence for extrachromosomal plasmid DNA in any of the strains examined. They also indicated that the Wolbachia genome is circular.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sun LV,Foster JM,Tzertzinis G,Ono M,Bandi C,Slatko BE,O'Neill SL

doi

10.1128/JB.183.7.2219-2225.2001

keywords:

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

2219-25

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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