In vitro analysis of rates and spectra of mutations in a polymorphic region of the Rv0746 PE_PGRS gene of Mycobacterium tuberculosis.

abstract：:Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

journal_title：Journal of bacteriology

authors： Golub EI,Low KB

更新日期：1985-04-01 00:00:00

abstract：:A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...

journal_title：Journal of bacteriology

authors： Shiga Y,Mizuno H,Akanuma H

更新日期：1993-11-01 00:00:00

abstract：:Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...

journal_title：Journal of bacteriology

authors： Pucci MJ,Daneo-Moore L

更新日期：1984-08-01 00:00:00

abstract：:Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

journal_title：Journal of bacteriology

authors： Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

更新日期：1984-10-01 00:00:00

abstract：:We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to...

journal_title：Journal of bacteriology

authors： Joseph E,Danchin A,Ullmann A

更新日期：1981-04-01 00:00:00

abstract：:To circumvent the permeability barrier of its outer membrane, Pseudomonas aeruginosa has evolved a series of specific porins. These channels have binding sites for related classes of molecules that facilitate uptake under nutrient-limited conditions. Here, we report on the identification of a 19-member family of porin...

journal_title：Journal of bacteriology

authors： Tamber S,Ochs MM,Hancock RE

更新日期：2006-01-01 00:00:00

abstract：:A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...

journal_title：Journal of bacteriology

authors： Kruyssen FJ,de Boer WR,Wouters JT

更新日期：1980-10-01 00:00:00

abstract：:Chlamydiae are unusual obligately intracellular bacteria that do not synthesize detectable peptidoglycan. However, they possess genes that appear to encode products with peptidoglycan biosynthetic activity. Bioinformatic analysis predicts that chlamydial MurE possesses UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelic acid (...

journal_title：Journal of bacteriology

authors： Patin D,Bostock J,Blanot D,Mengin-Lecreulx D,Chopra I

更新日期：2009-12-01 00:00:00

abstract：:Mycobacterial genomes are endowed with many eukaryote-like nucleotide cyclase genes encoding proteins that can synthesize 3',5'-cyclic AMP (cAMP). However, the roles of cAMP and the need for such redundancy in terms of adenylyl cyclase genes remain unknown. We measured cAMP levels in Mycobacterium smegmatis during gro...

journal_title：Journal of bacteriology

authors： Dass BK,Sharma R,Shenoy AR,Mattoo R,Visweswariah SS

更新日期：2008-06-01 00:00:00

abstract：:Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caul...

journal_title：Journal of bacteriology

authors： Yeh YC,Comolli LR,Downing KH,Shapiro L,McAdams HH

更新日期：2010-10-01 00:00:00

abstract：:Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage u...

journal_title：Journal of bacteriology

authors： Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G

更新日期：2008-04-01 00:00:00

abstract：:Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Detection and persistence of Vi antigen in tissues of actively immunized mice. J. Bacteriol. 89:776-781. 1965.-The presence, distribution, and persistence of Vi antigen in mouse tissue was determined by me...

journal_title：Journal of bacteriology

authors： GAINES S,CURRIE JA,TULLY JG

更新日期：1965-03-01 00:00:00

abstract：:The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

journal_title：Journal of bacteriology

authors： Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

更新日期：1997-06-01 00:00:00

abstract：:Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...

journal_title：Journal of bacteriology

authors： ISHIBASHI M,SUGINO Y,HIROTA Y

更新日期：1964-03-01 00:00:00

abstract：:The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

journal_title：Journal of bacteriology

authors： Anderson DG,Moseley SL

更新日期：1988-10-01 00:00:00

abstract：:MalT is the central transcriptional activator of all mal genes in Escherichia coli. Its activity is controlled by the inducer maltotriose. It can be inhibited by the interaction with certain proteins, and its expression can be controlled. We report here a novel aspect of mal gene regulation: the effect of cytoplasmic ...

journal_title：Journal of bacteriology

authors： Lengsfeld C,Schönert S,Dippel R,Boos W

更新日期：2009-02-01 00:00:00

abstract：:Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...

journal_title：Journal of bacteriology

authors： Price LB,Shand RF

更新日期：2000-09-01 00:00:00

abstract：:Sigma 54 associates with bacterial core RNA polymerase and converts it into an enhancer-responsive enzyme. Deletion of the N-terminal 40 amino acids is known to result in loss of the ability to respond to enhancer binding proteins. In this work PCR mutagenesis and genetic screens were used to identify a small patch, f...

journal_title：Journal of bacteriology

authors： Syed A,Gralla JD

更新日期：1998-11-01 00:00:00

abstract：:The mevalonate diphosphate decarboxylase is an enzyme which converts mevalonate diphosphate to isopentenyl diphosphate, the building block of isoprenoids. We used the Saccharomyces cerevisiae temperature-sensitive mutant defective for mevalonate diphosphate decarboxylase previously described (C. Chambon, V. Ladeveve, ...

journal_title：Journal of bacteriology

authors： Bergès T,Guyonnet D,Karst F

更新日期：1997-08-01 00:00:00

abstract：:Aldrich, K. M. (The University of Kansas, Lawrence), and C. P. Sword. Methicillin-induced lysozyme-sensitive forms of staphylococci. J. Bacteriol. 87:690-695. 1964.-Staphylococcus aureus and S. epidermidis grown in the presence of sublethal amounts of methicillin were converted to enlarged spheres within 2 to 4 hr, as...

journal_title：Journal of bacteriology

authors： ALDRICH KM,SWORD CP

更新日期：1964-03-01 00:00:00

abstract：:PomA is thought to be a component of the ion channel in the sodium-driven polar-flagellar motor of Vibrio alginolyticus. We have found that some cysteine substitutions in the periplasmic region of PomA result in a slow-motility phenotype, in which swarming and swimming speeds are reduced even in the presence of high c...

journal_title：Journal of bacteriology

authors： Kojima S,Shoji T,Asai Y,Kawagishi I,Homma M

更新日期：2000-06-01 00:00:00

abstract：:The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that...

journal_title：Journal of bacteriology

authors： Saito Y,Uraki F,Nakajima S,Asaeda A,Ono K,Kubo K,Yamamoto K

更新日期：1997-06-01 00:00:00

abstract：:A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...

journal_title：Journal of bacteriology

authors： Schecter JB

更新日期：1983-06-01 00:00:00

abstract：:Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need fur...

journal_title：Journal of bacteriology

authors： Le Laz S,Le Goaziou A,Henneke G

更新日期：2010-07-01 00:00:00

abstract：:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

journal_title：Journal of bacteriology

authors： Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

更新日期：2000-03-01 00:00:00

abstract：:Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...

journal_title：Journal of bacteriology

authors： Kjellin-Stråby K

更新日期：1969-11-01 00:00:00

abstract：:Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J. Bacteriol. 91:609-616. 1966.-Lipid composition of Mycoplasma laidlawii membranes could be significantly changed by var...

journal_title：Journal of bacteriology

authors： Razin S,Tourtellotte ME,McElhaney RN,Pollack JD

更新日期：1966-02-01 00:00:00

abstract：:A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

journal_title：Journal of bacteriology

authors： Berg CM,Liu L,Wang B,Wang MD

更新日期：1988-01-01 00:00:00

abstract：:We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

journal_title：Journal of bacteriology

authors： Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

更新日期：1985-01-01 00:00:00

abstract：:Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2...

journal_title：Journal of bacteriology

authors： Chang YF,Feingold DS

更新日期：1970-04-01 00:00:00