Regulation of galactose operon expression: glucose effects and role of cyclic adenosine 3',5'-monophosphate.

Abstract:

:We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to exhibit catabolite repression. The only glucose effect on galactose operon expression which we observed was inducer exclusion, as shown by the existence of diauxic growth in the presence of glucose and galactose. This diauxie was not relieved by cyclic adenosine 3',5'-monophosphate. Cyclic adenosine 3',5'-monophosphate did not seem to be an antagonist of any glucose effect on galactose operon expression; its only effect was to stimulate promoter-distal gene expression.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Joseph E,Danchin A,Ullmann A

doi

10.1128/JB.146.1.149-154.1981

subject

Has Abstract

pub_date

1981-04-01 00:00:00

pages

149-54

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

146

pub_type

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