HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum.

Abstract:

:Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small protein that accumulated under iron limitation and is transcriptionally controlled by the global iron-responsive regulator Irr, as were all genes within the heme utilization locus. Cross-linking and immunoprecipitation experiments showed that Irr occupies the hmuR-hmuP promoter in vivo. An hmuP mutant did not grow on heme as an iron source, but retained the ability to use ferric chloride. Correspondingly, induction of hmuR mRNA under iron limitation was severely diminished in an hmuP strain, but other genes within the Irr regulon were unaffected. HmuP occupied the hmuR-hmuP promoter, and thus it plays a direct regulatory role in gene expression. HmuP was not required for Irr occupancy, nor was ectopic expression of hmuP from an Irr-independent promoter sufficient to induce the hmuR gene. Thus, both HmuP and Irr occupancy are necessary for hmuR induction. We suggest that HmuP is a coactivator of Irr-dependent expression of hmuR.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Escamilla-Hernandez R,O'Brian MR

doi

10.1128/JB.00071-12

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

3137-43

issue

12

eissn

0021-9193

issn

1098-5530

pii

JB.00071-12

journal_volume

194

pub_type

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