Genetic analysis of nonpathogenic Agrobacterium tumefaciens mutants arising in crown gall tumors.

Abstract:

:Little is known about the effect of the host on the genetic stability of bacterial plant pathogens. Crown gall, a plant disease caused by Agrobacterium tumefaciens, may represent a useful model to study this effect. Indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria suggest that the host plant might induce loss of pathogenicity in populations of A. tumefaciens. Here we report that five different A. tumefaciens strains initially isolated from apple tumors produced up to 99% nonpathogenic mutants following their reintroduction into axenic apple plants. Two of these five strains were also found to produce mutants on pear and/or blackberry plants. Generally, the mutants of the apple isolate D10B/87 were altered in the tumor-inducing plasmid, harboring either deletions in this plasmid or point mutations in the regulatory virulence gene virG. Most of the mutants originating from the same tumor appeared to be of clonal origin, implying that the host plants influenced agrobacterial populations by favoring growth of nonpathogenic mutants over that of wild-type cells. This hypothesis was confirmed by coinoculation of apple rootstocks with strain D10B/87 and a nonpathogenic mutant.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bélanger C,Canfield ML,Moore LW,Dion P

doi

10.1128/jb.177.13.3752-3757.1995

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

3752-7

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

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