Reactivative action of ethylenediaminetetraacetic acid or dipicolinic acid on inactive glucose dehydrogenase obtained from heated spores of Bacillus subtilis.

Abstract:

:Partially purified inactive glucose dehydrogenase obtained from spores which were heated at 87 or 90 C for 30 min is converted to an active from by the addition of ethylenediaminetetraacetic acid, dipicolinic acid, or some salts. The molecular weight of the inactive glucose dehydrogenase in the heated spores is about one-half of that of the active glucose dehydrogenase in the intact resting spores. The possibility is discussed that the active glucose dehydrogenase in the intact resting spores divides into subunits and is converted to stable and inactive form during heating of spores at a particular range of temperature (87 to 90 C).

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hachisuka Y,Tochikubo K

doi

10.1128/JB.107.2.442-447.1971

subject

Has Abstract

pub_date

1971-08-01 00:00:00

pages

442-7

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

107

pub_type

杂志文章
  • Polar targeting of DivIVA in Bacillus subtilis is not directly dependent on FtsZ or PBP 2B.

    abstract::DivIVA is involved in Bacillus subtilis cell division and is located at the cell poles. Previous experiments suggested that the cell division proteins FtsZ and PBP 2B are required for polar targeting of DivIVA. By using outgrowing spores, we show that DivIVA accumulates at the cell poles independent of the presence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.2.693-697.2003

    authors: Hamoen LW,Errington J

    更新日期:2003-01-01 00:00:00

  • A novel branching enzyme of the GH-57 family in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

    abstract::Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00390-06

    authors: Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

    更新日期:2006-08-01 00:00:00

  • Control of transcription of gal repressor and isorepressor genes in Escherichia coli.

    abstract::Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.1.251-258.1993

    authors: Weickert MJ,Adhya S

    更新日期:1993-01-01 00:00:00

  • Glycine betaine catabolism contributes to Pseudomonas syringae tolerance to hyperosmotic stress by relieving betaine-mediated suppression of compatible solute synthesis.

    abstract::Many bacteria can accumulate glycine betaine for osmoprotection and catabolize it as a growth substrate, but how they regulate these opposing roles is poorly understood. In Pseudomonas syringae B728a, expression of the betaine catabolism genes was reduced by an osmotic upshift to an intermediate stress level, consiste...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00094-13

    authors: Li S,Yu X,Beattie GA

    更新日期:2013-05-01 00:00:00

  • Ultraviolet microscopy of purines and amino acids in the vacuole of Candida albicans.

    abstract::Ultraviolet (UV) microscopy was used to study the capacity of yeast (ATCC 10231 and 10261) and filamentous (ATCC 10259) strains of Candida albicans to accumulate UV-absorbing materials from a medium supplemented with purines, pyrimidines, amino acids, or related compounds as the main nitrogen source. All strains accum...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.1.259-265.1968

    authors: Balish E,Svihla G

    更新日期:1968-07-01 00:00:00

  • Purification and biochemical characterization of tellurite-reducing activities from Thermus thermophilus HB8.

    abstract::Cell-free extracts of Thermus thermophilus HB8 catalyze the in vitro, NADH-dependent reduction of potassium tellurite (K2TeO3). Three different protein fractions with tellurite-reducing activities were identified. Two exhibited high molecular weight and were composed of at least two different polypeptides. The protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3269-3273.1988

    authors: Chiong M,González E,Barra R,Vásquez C

    更新日期:1988-07-01 00:00:00

  • Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.

    abstract::Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.1.146-154.1979

    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

  • Plasmids in avirulent strains of Agrobacterium.

    abstract::Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.76-80.1977

    authors: Merlo DJ,Nester EW

    更新日期:1977-01-01 00:00:00

  • Regulation of Bacillus subtilis sigmaH (spo0H) and AbrB in response to changes in external pH.

    abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6778-6787.1997

    authors: Cosby WM,Zuber P

    更新日期:1997-11-01 00:00:00

  • Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima.

    abstract::Edwards, P. R. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), R. Sakazaki, and I. Kato. Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima. J. Bacteriol. 84:99-103. 1962-Study of cultures of Salmonella mikawashima isolated from reptiles revealed that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.1.99-103.1962

    authors: EDWARDS PR,SAKAZAKI R,KATO I

    更新日期:1962-07-01 00:00:00

  • The final step of peptidoglycan subunit assembly in Escherichia coli occurs in the cytoplasm.

    abstract::The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.6.1841-1843.1993

    authors: Bupp K,van Heijenoort J

    更新日期:1993-03-01 00:00:00

  • Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase.

    abstract::The discovery of a novel multicistronic operon that encodes phosphofructokinase, pyruvate kinase, and lactate dehydrogenase in the lactic acid bacterium Lactococcus lactis is reported. The three genes in the operon, designated pfk, pyk, and ldh, contain 340, 502, and 325 codons, respectively. The intergenic distances ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2541-2551.1993

    authors: Llanos RM,Harris CJ,Hillier AJ,Davidson BE

    更新日期:1993-05-01 00:00:00

  • Ultrastructure of Azotobacter vinelandii.

    abstract::Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.933-939.1970

    authors: Vela GR,Cagle GD,Holmgren PR

    更新日期:1970-11-01 00:00:00

  • Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: organization of the tar region.

    abstract::The tar locus of Escherichia coli specifies one of the major species of methyl-accepting proteins involved in the chemotactic behavior of this organism. The physical and genetic organization of the tar region was investigated with a series of specialized lambda transducing phages and plasmid clones. The tar gene was m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.565-577.1983

    authors: Slocum MK,Parkinson JS

    更新日期:1983-08-01 00:00:00

  • Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1.

    abstract::The genes coding for two structurally different isocitrate dehydrogenase isozymes (IDH-I and IDH-II) of a psychrophilic bacterium, Vibrio sp. strain ABE-1, were cloned and sequenced. Open reading frames of the genes (icdI and icdII) are 1,248 and 2,229 bp in length, respectively. The amino acid sequences predicted fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.21.6873-6880.1993

    authors: Ishii A,Suzuki M,Sahara T,Takada Y,Sasaki S,Fukunaga N

    更新日期:1993-11-01 00:00:00

  • Carbon monoxide metabolism of the methylotrophic acidogen Butyribacterium methylotrophicum.

    abstract::The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multip...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.1.255-263.1982

    authors: Lynd L,Kerby R,Zeikus JG

    更新日期:1982-01-01 00:00:00

  • Identification of an Escherichia coli operon required for formation of the O-antigen capsule.

    abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5259-5266.2005

    authors: Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

    更新日期:2005-08-01 00:00:00

  • Molecular cloning and transcriptional analysis of a guanosine kinase gene of Brevibacterium acetylicum ATCC 953.

    abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.6959-6964.1997

    authors: Usuda Y,Kawasaki H,Shimaoka M,Utagawa T

    更新日期:1997-11-01 00:00:00

  • Vibrio cholerae VibF is required for vibriobactin synthesis and is a member of the family of nonribosomal peptide synthetases.

    abstract::A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.6.1731-1738.2000

    authors: Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

    更新日期:2000-03-01 00:00:00

  • Effects of pancreatin on the growth of reovirus.

    abstract::Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Joseph L. Melnick, and Fred Rapp. Effects of pancreatin on the growth of reovirus. J. Bacteriol. 92:155-160. 1966.-The influence of pancreatin and other proteolytic enzymes on the growth, plaque formation, and antigenicity of reovirus was studied. S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.1.155-160.1966

    authors: Wallis C,Melnick JL,Rapp F

    更新日期:1966-07-01 00:00:00

  • Complete genome sequence of the veterinary pathogen Staphylococcus pseudintermedius strain HKU10-03, isolated in a case of canine pyoderma.

    abstract::Staphylococcus pseudintermedius is a member of the coagulase-positive staphylococci and is the commonest cause of canine pyoderma. We report the first genome sequence of S. pseudintermedius, which shows the presence of numerous virulence factors akin to those of the related human pathogen Staphylococcus aureus. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00023-11

    authors: Tse H,Tsoi HW,Leung SP,Urquhart IJ,Lau SK,Woo PC,Yuen KY

    更新日期:2011-04-01 00:00:00

  • Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters.

    abstract::We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.17.5516-5520.1999

    authors: Kato A,Tanabe H,Utsumi R

    更新日期:1999-09-01 00:00:00

  • Utilization of nitrate by pathogenic and saprophytic mycobacteria.

    abstract::Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.2.195-205.1962

    authors: HEDGECOCK LW,COSTELLO RL

    更新日期:1962-08-01 00:00:00

  • Cell division of cycle of Bacillus subtilis: evidence of variability in period D.

    abstract::In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.254-261.1980

    authors: Holmes M,Rickert M,Pierucci O

    更新日期:1980-04-01 00:00:00

  • Modulation of anaerobic energy metabolism of Bacillus subtilis by arfM (ywiD).

    abstract::Bacillus subtilis grows under anaerobic conditions utilizing nitrate ammonification and various fermentative processes. The two-component regulatory system ResDE and the redox regulator Fnr are the currently known parts of the regulatory system for anaerobic adaptation. Mutation of the open reading frame ywiD located ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.23.6815-6821.2001

    authors: Marino M,Ramos HC,Hoffmann T,Glaser P,Jahn D

    更新日期:2001-12-01 00:00:00

  • Functional and structural analysis of HicA3-HicB3, a novel toxin-antitoxin system of Yersinia pestis.

    abstract::The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01932-14

    authors: Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

    更新日期:2014-11-01 00:00:00

  • An extreme thermophile, Thermus thermophilus, is a polyploid bacterium.

    abstract::An extremely thermophilic bacterium, Thermus thermophilus HB8, is one of the model organisms for systems biology. Its genome consists of a chromosome (1.85 Mb), a megaplasmid (0.26 Mb) designated pTT27, and a plasmid (9.3 kb) designated pTT8, and the complete sequence is available. We show here that T. thermophilus is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00662-10

    authors: Ohtani N,Tomita M,Itaya M

    更新日期:2010-10-01 00:00:00

  • Regions of Salmonella typhimurium flagellin essential for its polymerization and excretion.

    abstract::Immunological methods were used to examine the flagellin production of Salmonella typhimurium strains that carried a mutation in one of the two possible genes for flagellin (H1 or H2) and also were incapable of expressing the other gene. Some mutants produced flagellin that was excreted into the culture medium; others...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.291-296.1987

    authors: Homma M,Fujita H,Yamaguchi S,Iino T

    更新日期:1987-01-01 00:00:00

  • Valinomycin-induced cation transport in vesicles does not reflect the activity of K+ transport systems in Escherichia coli.

    abstract::Transport systems for K+ in Escherichia coli are not detectable in membrane vesicles, but vesicles will take up K+ (and Rb+) in the presence of valinomycin. It is generally believed that valinomycin acts as a lipid-soluble cation carrier and that it does not interact with or activate cation transport systems. This vie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.334-337.1986

    authors: Altendorf K,Epstein W,Löhmann A

    更新日期:1986-04-01 00:00:00

  • Molecular cloning and characterization of two genes for the biotin carboxylase and carboxyltransferase subunits of acetyl coenzyme A carboxylase in Myxococcus xanthus.

    abstract::We have cloned a DNA fragment from a genomic library of Myxococcus xanthus using an oligonucleotide probe representing conserved regions of biotin carboxylase subunits of acetyl coenzyme A (acetyl-CoA) carboxylases. The fragment contained two open reading frames (ORF1 and ORF2), designated the accB and accA genes, cap...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.19.5462-5469.2000

    authors: Kimura Y,Miyake R,Tokumasu Y,Sato M

    更新日期:2000-10-01 00:00:00