Abstract:
:The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multiple transfers in the absence of CO. CO dehydrogenase activity was lower in the CO strain grown on CO (13.3 micromol/min per mg of protein) than in the Marburg strain grown on methanol-acetate (47.2 mumol/min per mg of protein); thus, the levels of this enzyme did not explain the growth on CO. CO was dissimilated to acetate and CO2 with the following stoichiometry: 4 CO leads to 2.17 CO2 + 0.74 acetate. We observed a growth rate of 0.05 h-1, a final optical density at 660 nm of 0.8, and a cell yield of 3.0 g of cells per mol of CO during growth of the CO strain. Growing cultures of the CO strain displayed a Ks for CO of 28 to 56 microM. The apparent thermodynamic efficiency of cell synthesis from CO was 57%. Energetic and biochemical aspects of CO metabolism are described.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lynd L,Kerby R,Zeikus JGdoi
10.1128/JB.149.1.255-263.1982subject
Has Abstractpub_date
1982-01-01 00:00:00pages
255-63issue
1eissn
0021-9193issn
1098-5530journal_volume
149pub_type
杂志文章abstract::We have investigated the mechanism of osmotic stress adaptation (osmoregulation) in Agrobacterium tumefaciens biotype I (salt-tolerant) and biotype II (salt-sensitive) strains. Using natural-abundance 13C nuclear magnetic resonance spectroscopy, we identified all organic solutes that accumulated to significant levels ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.12.6849-6855.1990
更新日期:1990-12-01 00:00:00
abstract::The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.361-364.1970
更新日期:1970-02-01 00:00:00
abstract::The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.787-792.1968
更新日期:1968-03-01 00:00:00
abstract::The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C. Kündig, H. Hennecke, and M. Göttfert, J. Bacteriol. 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein NifA. Southern blots of restriction enzyme-digested cosmids that represent a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1472-1480.2000
更新日期:2000-03-01 00:00:00
abstract::The 2-aminoethylphosphonate transaminase (AEPT; the phnW gene product) of the Salmonella enterica serovar Typhimurium 2-aminoethylphosphonate (AEP) degradation pathway catalyzes the reversible reaction of AEP and pyruvate to form phosphonoacetaldehyde (P-Ald) and L-alanine (L-Ala). Here, we describe the purification a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.15.4134-4140.2002
更新日期:2002-08-01 00:00:00
abstract::In Escherichia coli, the GlpT transporter, a member of the major facilitator superfamily, moves external glycerol 3-phosphate (G3P) into the cytoplasm in exchange for cytoplasmic phosphate. Study of intact cells showed that both GlpT and HisGlpT, a variant with an N-terminal six-histidine tag, are inhibited (50% inhib...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3863-3870.2003
更新日期:2003-07-01 00:00:00
abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
更新日期:1990-07-01 00:00:00
abstract::Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05331-11
更新日期:2011-09-01 00:00:00
abstract::Three homofermentative (Lactobacillus plantarum B38, L. plantarum B33, Pediococcus pentosaceus B30) and three heterofermentative (Leuconostoc mesenteroides 39, L. oenos B70, Lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (NAD)-dependent and NAD...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1284-1289.1971
更新日期:1971-12-01 00:00:00
abstract::Biofilm formation was repressed by glucose in several species of Enterobacteriaceae. In Escherichia coli, this effect was mediated at least in part by cyclic AMP (cAMP)-cAMP receptor protein. A temporal role for cAMP in biofilm development was indicated by the finding that glucose addition after approximately 24 h fai...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3406-3410.2002
更新日期:2002-06-01 00:00:00
abstract::Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.424-435.1979
更新日期:1979-11-01 00:00:00
abstract::Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.418-423.1966
更新日期:1966-08-01 00:00:00
abstract::Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.446-453.1964
更新日期:1964-02-01 00:00:00
abstract::The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00853-10
更新日期:2010-12-01 00:00:00
abstract::The pattern of peptidoglycan (murein) segregation in cells of Escherichia coli with impaired activity of the morphogenetic proteins penicillin-binding protein 2 and RodA has been investigated by the D-cysteine-biotin immunolabeling technique (M. A. de Pedro, J. C. Quintela, J.-V. Höltje, and H. Schwarz, J. Bacteriol. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.14.4115-4126.2001
更新日期:2001-07-01 00:00:00
abstract::The frizzy (frz) genes of Myxococcus xanthus are required to control directed motility during vegetative growth and fruiting body formation. FrzCD, a protein homologous to the methyl-accepting chemotaxis proteins from enteric bacteria, is modified by methylation in response to environmental conditions. Transfer of cel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4936-4940.1993
更新日期:1993-08-01 00:00:00
abstract::Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody. J. Bacteriol. 92:1741-1747. 1966.-The severity of persistent herpes simplex virus (HSV) infections maintained in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.6.1741-1747.1966
更新日期:1966-12-01 00:00:00
abstract::The genome of the facultative ribulose monophosphate (RuMP) cycle methylotroph Bacillus methanolicus encodes two bisphosphatases (GlpX), one on the chromosome (GlpX(C)) and one on plasmid pBM19 (GlpX(P)), which is required for methylotrophy. Both enzymes were purified from recombinant Escherichia coli and were shown t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00672-13
更新日期:2013-11-01 00:00:00
abstract::Surface protein antigens of Mycoplasma hyopneumoniae were identified by direct antibody-surface binding or by radioimmunoprecipitation of surface 125I-labeled proteins with a series of monoclonal antibodies (MAbs). Surface proteins p70, p65, p50, and p44 were shown to be integral membrane components by selective parti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5546-5555.1987
更新日期:1987-12-01 00:00:00
abstract::Duerre, John A. (University of North Dakota, Grand Forks), and Patrick J. Buckley. Pigment production from tryptophan by an Achromobacter species. J. Bacteriol. 90:1686-1691. 1965.-A microorganism was isolated from the soil near the University of North Dakota. Biochemical and morphological characteristics indicated th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.6.1686-1691.1965
更新日期:1965-12-01 00:00:00
abstract::The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4890-4896.1988
更新日期:1988-10-01 00:00:00
abstract::Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4230-4237.1995
更新日期:1995-08-01 00:00:00
abstract::The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::The reoxidation of NADH generated in reactions within the mitochondrial matrix of Saccharomyces cerevisiae is catalyzed by an NADH dehydrogenase designated Ndi1p (C. A. M. Marres, S. de Vries, and L. A. Grivell, Eur. J. Biochem. 195:857-862, 1991). Gene disruption analysis was used to examine possible metabolic functi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.16.4051-4055.1998
更新日期:1998-08-01 00:00:00
abstract::An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01433-10
更新日期:2011-06-01 00:00:00
abstract::Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.611-618.1996
更新日期:1996-02-01 00:00:00
abstract::Rohlfing, S. R. (Western Reserve University, Cleveland, Ohio), and I. P. Crawford. Purification and characterization of the beta-galactosidase of Aeromonas formicans. J. Bacteriol. 91:1085-1097. 1966.-The beta-galactosidase of Aeromonas formicans was purified by diethylaminoethyl cellulose chromatography and gel filtr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1085-1097.1966
更新日期:1966-03-01 00:00:00
abstract::The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. py...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6960-6967.1996
更新日期:1996-12-01 00:00:00
abstract::The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.30-35.1970
更新日期:1970-04-01 00:00:00
abstract::Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.5-8.1985
更新日期:1985-04-01 00:00:00