Abstract:
:Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococcal sensor protein gene. In this study, the DNA surrounding the PCR product origin was cloned and sequenced. This analysis revealed the presence of two genes, termed lytS and lytR, whose deduced amino acid sequences were similar to those of members of the two-component regulatory system family of proteins. S. aureus cells containing an insertional disruption of lytS exhibited a marked propensity to form aggregates in liquid culture, suggesting that alterations in cell surface components exist in this strain. Transmission electron microscopic examination of these cells revealed that the cell surface was rough and diffuse and that a large proportion of the cell population had lysed. The lytS mutant also exhibited increased autolysis and an altered level of murein hydrolase activity produced compared with the parental strain, NCTC 8325-4. These data suggest that the lytS and lytR gene products control the rate of autolysis in S. aureus by affecting the intrinsic murein hydrolase activity associated with the cell.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Brunskill EW,Bayles KWdoi
10.1128/jb.178.3.611-618.1996subject
Has Abstractpub_date
1996-02-01 00:00:00pages
611-8issue
3eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05048-11
更新日期:2011-07-01 00:00:00
abstract::Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompeten...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.360-362.1970
更新日期:1970-10-01 00:00:00
abstract::Quorum sensing control mediated by N-acyl homoserine lactone (AHL) signaling molecules has been established as a key feature of the regulation of exoenzyme production in many gram-negative bacteria. In Chromobacterium violaceum ATCC 31532 a number of phenotypic characteristics, including production of the purple pigme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.17.4435-4441.1998
更新日期:1998-09-01 00:00:00
abstract::In contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrAB) of Desulfobacula toluolica were vertically inherited. Furthermore, Desulfobacterium anilini and strain mXyS1 were identified, by dsrAB sequencing of 17 reference strains, as members of the donor lineage for thos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.6.2203-2208.2005
更新日期:2005-03-01 00:00:00
abstract::Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1390-1394.1969
更新日期:1969-06-01 00:00:00
abstract::The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.8.2737-2746.2005
更新日期:2005-04-01 00:00:00
abstract::Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.1.162-170.1964
更新日期:1964-01-01 00:00:00
abstract::Genetic studies have identified a specificity domain for prohead binding in the C-terminal 32 amino acids of gpA, the large subunit of bacteriophage lambda terminase (S. Frackman, D. A. Siegele, and M. Feiss, J. Mol. Biol. 180:283-300, 1984). In the present work, an amber mutation, Aam42, in the fifth-to-last codon of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.850-856.1992
更新日期:1992-02-01 00:00:00
abstract::Freeman, Bob A. (University of Chicago, Chicago, Ill.) and Richard Circo. Effect of tetracyclines on the intracellular amino acids of molds. J. Bacteriol. 86:38-44. 1963.-The tetracycline antibiotics were shown to alter the amino acid metabolism of molds whose growth is not markedly affected. Eight molds were grown in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.1.38-44.1963
更新日期:1963-07-01 00:00:00
abstract::Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.628-633.1966
更新日期:1966-02-01 00:00:00
abstract::Specific changes in the chemical and microbial composition of Thermoanaerobium brockii fermentations were compared and related to alterations of process rates, end product yields, and growth parameters. Fermentation of starch as compared with glucose was associated with significant decreases in growth rate and intrace...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.192-199.1981
更新日期:1981-04-01 00:00:00
abstract::The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.398-400.1991
更新日期:1991-01-01 00:00:00
abstract::The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1393-1401.1969
更新日期:1969-12-01 00:00:00
abstract::ParB is one of two P1-encoded proteins that are required for active partition of the P1 prophage in Escherichia coli. To probe the native domain structure of ParB, we performed limited proteolytic digestions of full-length ParB, as well as of several N-terminal and C-terminal deletion fragments of ParB. The C-terminal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.5898-5908.1999
更新日期:1999-10-01 00:00:00
abstract::Mycobacterium avium secretes iron-binding siderophores called exochelins. The exochelins from M. avium have previously been reported to have unsaturated side chains that terminate in carboxylic acid. In contrast, our data show the side chains to be both saturated and unsaturated and to terminate with either a carboxyl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6394-6398.1996
更新日期:1996-11-01 00:00:00
abstract::The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.980-982.1968
更新日期:1968-03-01 00:00:00
abstract::Shiio, Tsuru (Washington State University, Pullman), and Bruce A. McFadden. Cell-free amino acid-incorporating system from Pseudomonas indigofera. J. Bacteriol. 90:978-983. 1965.-A cell-free preparation from Pseudomonas indigofera incorporated C(14)-phenylalanine and C(14)-leucine into a product which was insoluble in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.4.978-983.1965
更新日期:1965-10-01 00:00:00
abstract::Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.6.1503-1513.2002
更新日期:2002-03-01 00:00:00
abstract::Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1315-1320.1987
更新日期:1987-03-01 00:00:00
abstract::The mutualistic symbiont Vibrio fischeri builds a symbiotic biofilm during colonization of squid hosts. Regulation of the exopolysaccharide component, termed Syp, has been examined in strain ES114, where production is controlled by a phosphorelay that includes the inner membrane hybrid histidine kinase RscS. Most stra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00033-19
更新日期:2019-04-09 00:00:00
abstract::Aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type II secretory pathway or secreton. ExeA is an essential component of this system and is necessary for the localization and/or multimerization of the secretin ExeD. ExeA contains two sequence motifs characteris...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.18.6370-6378.2005
更新日期:2005-09-01 00:00:00
abstract::In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.413-420.1972
更新日期:1972-10-01 00:00:00
abstract::The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01121-07
更新日期:2007-12-01 00:00:00
abstract::The complex flagella of Rhizobium meliloti 2011 and MVII-1 were analyzed with regard to serology, fine structure, subunits, and amino acid composition. The serological identities of flagellar filaments of the two strains were demonstrated by double immunodiffusion with antiflagellin antiserum. The filaments had a diam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.361-366.1985
更新日期:1985-04-01 00:00:00
abstract::A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1731-1738.2000
更新日期:2000-03-01 00:00:00
abstract::We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01092-06
更新日期:2007-01-01 00:00:00
abstract::We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.1719-1724.1990
更新日期:1990-04-01 00:00:00
abstract::Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2031-2034.1968
更新日期:1968-06-01 00:00:00
abstract::We previously described 14 rare spontaneous morphological mutants of Candida albicans that were associated with chromosomal aberrations (E. P. Rustchenko-Bulgac, F. Sherman, and J. B. Hicks, J. Bacteriol. 172:1276-1283, 1990). Improved conditions for separation of chromosomes, as well as hybridization probes, were use...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.20.6586-6596.1991
更新日期:1991-10-01 00:00:00
abstract::We have determined the amounts of a number of small molecules and enzymes in the mother cell compartment and the developing forespore during sporulation of Bacillus megaterium. Significant amounts of adenosine 5'-triphosphate and reduced nicotinamide adenine dinucleotide were present in the forespore compartment befor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1130-1138.1977
更新日期:1977-06-01 00:00:00