Characterization of a zinc-containing alcohol dehydrogenase with stereoselectivity from the hyperthermophilic archaeon Thermococcus guaymasensis.

abstract：:The seven proteins encoded by the comG operon of Bacillus subtilis exhibit similarity to gene products required for the assembly of type 4 pili and for the secretion of certain proteins in gram-negative bacteria. Although polar transposon insertions in comG result in the loss of transformability and in the failure of ...

journal_title：Journal of bacteriology

authors： Chung YS,Dubnau D

更新日期：1998-01-01 00:00:00

abstract：:When Methanobacterium thermoautotrophicum cells were incubated in 50 mM potassium phosphate buffer (pH 7.0) containing 1 M sucrose and autolysate from Methanobacterium wolfei, they were transformed into protoplasts. The protoplasts, which possessed no cell wall, lysed in buffer without sucrose. Unlike whole cells, the...

journal_title：Journal of bacteriology

authors： Mountfort DO,Mörschel E,Beimborn DB,Schönheit P

更新日期：1986-11-01 00:00:00

abstract：:In aquatic environments, Caulobacter spp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features of Caulobacter crescentus colonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in...

journal_title：Journal of bacteriology

authors： Fiebig A

更新日期：2019-08-22 00:00:00

abstract：:Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.1a and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and ou...

journal_title：Journal of bacteriology

authors： Seale T

更新日期：1973-02-01 00:00:00

abstract：:Plasmid ColIb-P9 of the I alpha incompatibility group is known to encode a DNA primase that acts in the conjugal transfer of the plasmid and can substitute for mutant dnaG gene product in vegetative replication of the Escherichia coli chromosome. The relevant genetic determinant (sog) has previously been cloned into a...

journal_title：Journal of bacteriology

authors： Dalrymple BP,Boulnois GJ,Wilkins BM,Orr E,Williams PH

更新日期：1982-07-01 00:00:00

abstract：:Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...

journal_title：Journal of bacteriology

authors： Tucker AN,White DC

更新日期：1970-08-01 00:00:00

abstract：:The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...

journal_title：Journal of bacteriology

authors： Cui Y,Chatterjee A,Liu Y,Dumenyo CK,Chatterjee AK

更新日期：1995-09-01 00:00:00

abstract：:Penicillin-binding proteins (PBPs), although characterized from several organisms, have so far not been studied in mycobacteria. The present study is the first characterization of a PBP from Mycobacterium smegmatis. The PBP was purified by solubilization of the membranes with Triton X-100 and successive chromatography...

journal_title：Journal of bacteriology

authors： Basu J,Chattopadhyay R,Kundu M,Chakrabarti P

更新日期：1992-07-01 00:00:00

abstract：:Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined t...

journal_title：Journal of bacteriology

authors： Tanaka M,Harafuji H,Yamamoto T

更新日期：1982-08-01 00:00:00

abstract：:The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...

journal_title：Journal of bacteriology

authors： Kim YJ,Meyer RJ

更新日期：1991-09-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:Cells of Haemophilus influenzae strain Rd competent for genetic transformation irreversibly bound approximately five molecular fragments of H. influenzae deoxyribonucleic acid (DNA) per cell; under identical conditions, DNA derived from Escherichia coli B was not taken up (<1 molecule per 50 cells). Similarly, DNA fro...

journal_title：Journal of bacteriology

authors： Scocca JJ,Poland RL,Zoon KC

更新日期：1974-05-01 00:00:00

abstract：:Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...

journal_title：Journal of bacteriology

authors： Heidinger S,Braun V,Pecoraro VL,Raymond KN

更新日期：1983-01-01 00:00:00

abstract：:The 6th American Society for Microbiology Conference on Cell-Cell Communication in Bacteria convened from 16 to 19 October 2017 in Athens, GA. In this minireview, we highlight some of the research presented at that meeting that addresses central questions emerging in the field, including the following questions. How a...

journal_title：Journal of bacteriology

authors： Brown SP,Blackwell HE,Hammer BK

更新日期：2018-06-25 00:00:00

abstract：:Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...

journal_title：Journal of bacteriology

authors： Cook WR,MacAlister TJ,Rothfield LI

更新日期：1986-12-01 00:00:00

abstract：:A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...

journal_title：Journal of bacteriology

authors： Schecter JB

更新日期：1983-06-01 00:00:00

abstract：:We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...

journal_title：Journal of bacteriology

authors： Joyce CM,Grindley ND

更新日期：1984-05-01 00:00:00

abstract：:Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...

journal_title：Journal of bacteriology

authors： Crabtree S,Cronan JE Jr

更新日期：1984-04-01 00:00:00

abstract：:The chemical composition of the cell walls of several L-form revertants derived from Nocardia asteroides 10905 was determined at different stages of growth. It was observed that each L-form revertant had a cell well that differed from that of the parental strain when grown under identical conditions. In some strains t...

journal_title：Journal of bacteriology

authors： Beaman BL,Bourgeois AL,Moring SE

更新日期：1981-11-01 00:00:00

abstract：:The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4', 6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had one or two nucleoids in a cell, and no anucleate cells were found. The nucleoids were positioned in the center in mononu...

journal_title：Journal of bacteriology

authors： Seto S,Miyata M

更新日期：1999-10-01 00:00:00

abstract：:Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...

journal_title：Journal of bacteriology

authors： Estrada-Parra S,Zamora A,Bojalil LF

更新日期：1965-09-01 00:00:00

abstract：:We showed that the heat killing curve for exponentially growing Saccharomyces cerevisiae was biphasic. This suggests two populations of cells with different thermal killing characteristics. When exponentially growing cells separated into cell cycle-specific fractions via centrifugal elutriation were heat shocked, the ...

journal_title：Journal of bacteriology

authors： Plesset J,Ludwig JR,Cox BS,McLaughlin CS

更新日期：1987-02-01 00:00:00

abstract：:Cyanobacteria are monophyletic organisms that perform oxygenic photosynthesis. While they exhibit great diversity, they have a common set of genes. However, the essentiality of them for viability has hampered the elucidation of their functions. One example of these genes is cyabrB1 (also known as calA in Anabaena sp. ...

journal_title：Journal of bacteriology

authors： Higo A,Nishiyama E,Nakamura K,Hihara Y,Ehira S

更新日期：2019-08-08 00:00:00

abstract：:During the transition from a free-swimming, single-cell lifestyle to a sessile, multicellular state called a biofilm, bacteria produce and secrete an extracellular matrix comprised of nucleic acids, exopolysaccharides, and adhesion proteins. The Vibrio cholerae biofilm matrix contains three major protein components, R...

journal_title：Journal of bacteriology

authors： Giglio KM,Fong JC,Yildiz FH,Sondermann H

更新日期：2013-07-01 00:00:00

abstract：:Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...

journal_title：Journal of bacteriology

authors： Lubitz W,Harkness RE,Ishiguro EE

更新日期：1984-07-01 00:00:00

abstract：:Defective phage lambdadg, when present in certain Salmonella typhosa hybrids, could be eliminated with acridine orange or ethidium bromide treatment. The lambdadg deoxyribonucleic acid could be separated from the S. typhosa host deoxyribonucleic acid as a distinctly covalently closed molecule. ...

journal_title：Journal of bacteriology

authors： Falkow S,Baron LS

更新日期：1970-04-01 00:00:00

abstract：:Plasmids pRAS3.1 and pRAS3.2 are natural variants of the IncQ-2 plasmid family, that except for two differences, have identical plasmid backbones. Plasmid pRAS3.1 has four 22-bp iterons in its oriV region, while pRAS3.2 has only three 6-bp repeats and pRAS3.1 has five 6-bp repeats in the promoter region of the mobB-mo...

journal_title：Journal of bacteriology

authors： Loftie-Eaton W,Rawlings DE

更新日期：2010-12-01 00:00:00

abstract：:Riemerella anatipestifer is a Gram-negative, rod-shaped bacterium associated with epizootic infections in poultry. R. anatipestifer strain RA-YM, belonging to the serotype 1 prevalent in China, is a clinically isolated strain with high-level virulence. Here, we report the first genome sequence of this species. ...

journal_title：Journal of bacteriology

authors： Zhou Z,Peng X,Xiao Y,Wang X,Guo Z,Zhu L,Liu M,Jin H,Bi D,Li Z,Sun M

更新日期：2011-03-01 00:00:00

abstract：:AppppA and the DnaK protein have both been hypothesized to function in regulating the heat shock response of Escherichia coli. The proposals are that AppppA serves as a signal (alarmone) to turn on the heat shock response, whereas the DnaK protein is necessary to turn off the heat shock response. A simple model would ...

journal_title：Journal of bacteriology

authors： Bochner BR,Zylicz M,Georgopoulos C

更新日期：1986-11-01 00:00:00

abstract：:A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinat...

journal_title：Journal of bacteriology

authors： Edwards DL,Belsole DM,Guzik HJ,Unger BW

更新日期：1979-02-01 00:00:00