Escherichia coli DnaK protein possesses a 5'-nucleotidase activity that is inhibited by AppppA.

abstract：:Catabolism of nonphenolic arenes is frequently initiated by dioxygenases, yielding single isomer products with two adjacent hydroxylated asymmetric centers. The next enzymic reaction dehydrogenates these cyclic cis-diols, with aromatization yielding catechols for ring cleavage. There are two stereochemical questions t...

journal_title：Journal of bacteriology

authors： Morawski B,Casy G,Illaszewicz C,Griengl H,Ribbons DW

更新日期：1997-06-01 00:00:00

abstract：:The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

journal_title：Journal of bacteriology

authors： Smith WR,Yu I,Hungate RE

更新日期：1973-05-01 00:00:00

abstract：:Mutants of Anabaena sp. strain PCC 7120 that are incapable of sustained growth with air as the sole source of nitrogen were generated by using Tn5-derived transposons. Nitrogenase was expressed only in mutants that showed obvious morphological signs of heterocyst differentiation. Even under rigorously anaerobic condit...

journal_title：Journal of bacteriology

authors： Ernst A,Black T,Cai Y,Panoff JM,Tiwari DN,Wolk CP

更新日期：1992-10-01 00:00:00

abstract：:Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

journal_title：Journal of bacteriology

authors： Wolfe AD,Cook TM,Hahn FE

更新日期：1971-12-01 00:00:00

abstract：:Insertional mutagenesis was conducted on Bacillus subtilis cells to screen for mutants resistant to catabolite repression. Three classes of mutants that were resistant to glucose-promoted but not mannitol-promoted catabolite repression were identified. Cloning and sequencing of the mutated genes revealed that the muta...

journal_title：Journal of bacteriology

authors： Paulsen IT,Chauvaux S,Choi P,Saier MH Jr

更新日期：1998-02-01 00:00:00

abstract：:Willett, Norman P. (University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pa.), and Guy E. Morse. Long-chain fatty acid inhibition of growth of Streptococcus agalactiae in a chemically defined medium. J. Bacteriol. 91:2245-2250. 1966.-A chemically defined medium was developed for Streptococcus agal...

journal_title：Journal of bacteriology

authors： Willett NP,Morse GE

更新日期：1966-06-01 00:00:00

abstract：:In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...

journal_title：Journal of bacteriology

authors： Chen YE,Tsokos CG,Biondi EG,Perchuk BS,Laub MT

更新日期：2009-12-01 00:00:00

abstract：:Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...

journal_title：Journal of bacteriology

authors： Hoffmann HP,Szabo A,Avers CJ

更新日期：1970-10-01 00:00:00

abstract：UNLABELLED:Cell shape has been suggested to play an important role in the regulation of bacterial attachment to surfaces and the formation of communities associated with surfaces. We found that a cardiolipin synthase (Δcls) mutant of the rod-shaped bacterium Rhodobacter sphaeroides--in which synthesis of the anionic, h...

journal_title：Journal of bacteriology

authors： Lin TY,Santos TM,Kontur WS,Donohue TJ,Weibel DB

更新日期：2015-11-01 00:00:00

abstract：:To circumvent the permeability barrier of its outer membrane, Pseudomonas aeruginosa has evolved a series of specific porins. These channels have binding sites for related classes of molecules that facilitate uptake under nutrient-limited conditions. Here, we report on the identification of a 19-member family of porin...

journal_title：Journal of bacteriology

authors： Tamber S,Ochs MM,Hancock RE

更新日期：2006-01-01 00:00:00

abstract：:In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements...

journal_title：Journal of bacteriology

authors： Fröls S,Gordon PM,Panlilio MA,Duggin IG,Bell SD,Sensen CW,Schleper C

更新日期：2007-12-01 00:00:00

abstract：:The inhibition of growth of the K-12 strain of Escherichia coli by glycyl-l-leucine observed originally by Simmonds and co-workers was investigated. The inhibition was reversed by isoleucine and those precursors of isoleucine beyond threonine in the biosynthetic pathway. Threonine reversed the inhibition poorly. With ...

journal_title：Journal of bacteriology

authors： Vonder Haar RA,Umbarger HE

更新日期：1972-10-01 00:00:00

abstract：:The highly conserved DnaK chaperones consist of an N-terminal ATPase domain, a central substrate-binding domain, and a C-terminal domain whose function is not known. Since Bacillus subtilis dnaK was not able to complement an Escherichia coli dnaK null mutant, we performed domain element swap experiments to identify th...

journal_title：Journal of bacteriology

authors： Mogk A,Bukau B,Lutz R,Schumann W

更新日期：1999-03-01 00:00:00

abstract：:A genetic approach was undertaken to identify normal bacterial genes whose products function to limit the effective concentration of antibiotics. In this approach, a multicopy plasmid library containing cloned Escherichia coli chromosomal sequences was screened for transformants that showed increased resistance to a n...

journal_title：Journal of bacteriology

authors： Gambino L,Gracheck SJ,Miller PF

更新日期：1993-05-01 00:00:00

abstract：:A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. ...

journal_title：Journal of bacteriology

authors： Giddings TH,Cantino EC

更新日期：1974-11-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00

abstract：:Rotation of the bacterial flagellar motor is powered by a transmembrane gradient of protons or, in some species, sodium ions. The molecular mechanism of coupling between ion flow and motor rotation is not understood. The proteins most closely involved in motor rotation are MotA, MotB, and FliG. MotA and MotB are trans...

journal_title：Journal of bacteriology

authors： Zhou J,Sharp LL,Tang HL,Lloyd SA,Billings S,Braun TF,Blair DF

更新日期：1998-05-01 00:00:00

abstract：:Form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of the Calvin-Benson-Bassham cycle may be divided into two broad phylogenetic groups, referred to as red-like and green-like, based on deduced large subunit amino acid sequences. Unlike the form I enzyme from the closely related organism Rhodobacter spha...

journal_title：Journal of bacteriology

authors： Horken KM,Tabita FR

更新日期：1999-07-01 00:00:00

abstract：:Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...

journal_title：Journal of bacteriology

authors： Vales LD,Chase JW,Murphy JB

更新日期：1979-07-01 00:00:00

abstract：:To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

journal_title：Journal of bacteriology

authors： Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

更新日期：1997-11-01 00:00:00

abstract：:Transport systems for K+ in Escherichia coli are not detectable in membrane vesicles, but vesicles will take up K+ (and Rb+) in the presence of valinomycin. It is generally believed that valinomycin acts as a lipid-soluble cation carrier and that it does not interact with or activate cation transport systems. This vie...

journal_title：Journal of bacteriology

authors： Altendorf K,Epstein W,Löhmann A

更新日期：1986-04-01 00:00:00

abstract：:Glucose uptake by Bacteroides succinogenes S85 was measured under conditions that maintained anaerobiosis and osmotic stability. Uptake was inhibited by compounds which interfere with electron transport systems, maintenance of proton or metal ion gradients, or ATP synthesis. The most potent inhibitors were proton and ...

journal_title：Journal of bacteriology

authors： Franklund CV,Glass TL

更新日期：1987-02-01 00:00:00

abstract：:Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utiliz...

journal_title：Journal of bacteriology

authors： Lippincott JA,Beiderbeck R,Lippincott BB

更新日期：1973-10-01 00:00:00

abstract：:The formation of recombinants in Hfr crosses was studied in Escherichia coli strains carrying combinations of genes known to affect recombination and DNA repair. Mutations in ruv and recG eliminate activities that have been shown to process Holliday junction intermediates by nuclease cleavage and/or branch migration. ...

journal_title：Journal of bacteriology

authors： Ryder L,Whitby MC,Lloyd RG

更新日期：1994-03-01 00:00:00

abstract：:The genes for the beta, beta', and seven sigma factor subunits of RNA polymerase, for elongation factors EF-Tu1 and EF-Tu3, and for six rRNA operons were mapped on the combined genetic and physical map of the Streptomyces coelicolor chromosome. Like the previously mapped tRNA genes, the RNA polymerase and rRNA genes m...

journal_title：Journal of bacteriology

authors： van Wezel GP,Buttner MJ,Vijgenboom E,Bosch L,Hopwood DA,Kieser HM

更新日期：1995-01-01 00:00:00

abstract：:The DNA sequence of a 2,100-bp region containing the argE gene from Escherichia coli has been determined. The nucleotide sequence of the ppc-argE intergenic region was also solved and shown to contain six tandemly repeated REP sequences. Moreover, the oxyR gene has been mapped on the E. coli chromosome and shown to fl...

journal_title：Journal of bacteriology

authors： Meinnel T,Schmitt E,Mechulam Y,Blanquet S

更新日期：1992-04-01 00:00:00

abstract：:Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the...

journal_title：Journal of bacteriology

authors： Sardesai AA,Gowrishankar J

更新日期：2001-06-01 00:00:00

abstract：:Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...

journal_title：Journal of bacteriology

authors： Eberz G,Eitinger T,Friedrich B

更新日期：1989-03-01 00:00:00

abstract：:Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the ina...

journal_title：Journal of bacteriology

authors： Chandra TS,Friedrich CG

更新日期：1986-05-01 00:00:00

abstract：:An investigation of sulfate reduction in B tsnC*7004, a mutant of Escherichia coli lacking thioredoxin, is reported. Although thioredoxin is indispensable for the adenosine 3'-phosphate 5'-phosphosulfate (PAPS) sulfotransferase reaction under the usual conditions of assay in extracts of wild-type cells, the mutant gre...

journal_title：Journal of bacteriology

authors： Tsang ML,Schiff JA

更新日期：1978-04-01 00:00:00