Abstract:
:In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements. While no significant up-regulation of genes potentially involved in direct DNA damage reversal was observed, a specific transcriptional UV response involving 55 genes could be dissected. Although flow cytometry showed only modest perturbation of the cell cycle, strong modulation of the transcript levels of the Cdc6 replication initiator genes was observed. Up-regulation of an operon encoding Mre11 and Rad50 homologs pointed to induction of recombinational repair. Consistent with this, DNA double-strand breaks were observed between 2 and 8 h after UV treatment, possibly resulting from replication fork collapse at damaged DNA sites. The strong transcriptional induction of genes which potentially encode functions for pilus formation suggested that conjugational activity might lead to enhanced exchange of genetic material. In support of this, a statistical microscopic analysis demonstrated that large cell aggregates formed upon UV exposure. Together, this provided supporting evidence to a link between recombinational repair and conjugation events.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Fröls S,Gordon PM,Panlilio MA,Duggin IG,Bell SD,Sensen CW,Schleper Cdoi
10.1128/JB.01016-07subject
Has Abstractpub_date
2007-12-01 00:00:00pages
8708-18issue
23eissn
0021-9193issn
1098-5530pii
JB.01016-07journal_volume
189pub_type
杂志文章abstract::MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We al...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00371-10
更新日期:2010-09-01 00:00:00
abstract::Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01192-09
更新日期:2010-01-01 00:00:00
abstract::Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.1.180-185.1977
更新日期:1977-10-01 00:00:00
abstract::Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.9.2613-2628.1996
更新日期:1996-05-01 00:00:00
abstract::A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.122-131.1982
更新日期:1982-04-01 00:00:00
abstract::ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.18.5654-5656.2003
更新日期:2003-09-01 00:00:00
abstract::The heat shock response in Escherichia coli involves extensive induction of the heat shock proteins, with the concomitant suppression of the synthesis of the non-heat shock proteins. While the induction of the heat shock proteins has been shown to occur primarily at the transcriptional level, the suppression of non-he...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.743-748.1992
更新日期:1992-02-01 00:00:00
abstract::The hexC locus of Pseudomonas aeruginosa PAO1 was localized to a 247-bp segment of chromosomal DNA on the multicopy broad-host-range vector pRO1614. The presence of this plasmid (pPZ196) in strain PAO1 produced the so-called "hexC effect," a two- to ninefold increase in the activities of four carbohydrate catabolism e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4700-4709.1994
更新日期:1994-08-01 00:00:00
abstract::The intermolecular interactions of the mycobacteriophage Ms6 secretion chaperone with endolysin were characterized. The 384-amino-acid lysin (lysin(384))-binding domain was found to encompass the N-terminal region of Gp1, which is also essential for a lysis phenotype in Escherichia coli. In addition, a GXXXG-like moti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00380-11
更新日期:2011-09-01 00:00:00
abstract::The Escherichia coli gene cluster encoding enzymatic activities responsible for the synthesis and activation of 2,3-dihydroxybenzoic acid in the formation of the catechol siderophore enterobactin was localized to a 4.2-kilobase chromosomal DNA fragment. Analysis of various subclones and transposon insertion mutations ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4163-4170.1987
更新日期:1987-09-01 00:00:00
abstract::Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.320-322.1979
更新日期:1979-07-01 00:00:00
abstract::The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.237-244.1985
更新日期:1985-10-01 00:00:00
abstract::Expression of toxT, the transcription activator of cholera toxin and pilus production in Vibrio cholerae, is the consequence of a complex cascade of regulatory events that culminates in activation of the toxT promoter by TcpP and ToxR, two membrane-localized transcription factors. Both are encoded in operons with gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8309-8316.2004
更新日期:2004-12-01 00:00:00
abstract::In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3982-3984.1996
更新日期:1996-07-01 00:00:00
abstract::The metE gene of Salmonella typhimurium was cloned into plasmid pACYC184 by using a lambda gt7 -metE transducing phage as a source of metE DNA. The recombinant plasmid, designated pGS41 , carries a 12.8-kilobase-pair EcoRI insert fragment. The metE gene was subcloned from pGS41 into plasmid pBR322 on a 4.2-kilobase-pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.928-933.1984
更新日期:1984-06-01 00:00:00
abstract::A strain of Pseudomonas (P. putida HS1) was found to resemble P. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. The ability to degrade these substrates b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.952-964.1981
更新日期:1981-06-01 00:00:00
abstract:UNLABELLED:All methanofuran structural variants contain a basic core structure of 4-[N-(γ-l-glutamyl)-p-(β-aminoethyl)phenoxymethyl]-(aminomethyl)furan (APMF-Glu) but have different side chains depending on the source organism. Recently, we identified four genes (MfnA, MfnB, MfnC, and MfnD) that are responsible for the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00401-15
更新日期:2015-09-01 00:00:00
abstract::The pst gene cluster encodes the phosphate-specific transport (Pst) system. Inactivation of the Pst system constitutively activates the two-component regulatory system PhoBR and attenuates the virulence of pathogenic bacteria. In uropathogenic Escherichia coli strain CFT073, attenuation by inactivation of pst is predo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00168-17
更新日期:2017-11-14 00:00:00
abstract::The fruK gene encoding fructose-1-phosphate kinase (FruK), located within the fructose (fru)-catabolic operon of Rhodobacter capsulatus, was sequenced. FruK of R. capsulatus (316 amino acids; molecular weight = 31,232) is the same size as and is homologous to FruK of Escherichia coli, phosphofructokinase B (PfkB) of E...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3117-3127.1991
更新日期:1991-05-01 00:00:00
abstract::The mechanism by which iron uptake is facilitated by the siderophore rhodotorulic acid (RA) in the yeast Rhodotorula pilimanae was investigated with radioactively labeled Fe and RA and kinetically inert, chromic-substituted RA complexes. The weight of the evidence supports a model in which RA mediates iron transport t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.69-74.1978
更新日期:1978-10-01 00:00:00
abstract::The inner or cytoplasmic membrane fraction of the cell envelope of Escherichia coli was isolated by isopycnic centrifugation on sucrose gradients. The membrane proteins were analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels (8.5%), and up to 56 bands were resolved. Different preparations gave v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.947-953.1974
更新日期:1974-03-01 00:00:00
abstract::Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1144-1148.1977
更新日期:1977-06-01 00:00:00
abstract::In the microbial conversion of added hypoxanthine to 5'-inosinic acid, Mn(2+) concentration in the growth medium is known to have a profound effect both on the yield of 5'-inosinic acid and the morphology of cells of Brevibacterium ammoniagenes. To elucidate the mechanism in which Mn(2+) was concerned with cell morpho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1760-1767.1968
更新日期:1968-11-01 00:00:00
abstract::In Listeria monocytogenes, the full details of how stress signals are integrated into the σB regulatory pathway are not yet available. To help shed light on this question, we investigated a collection of transposon mutants that were predicted to have compromised activity of the alternative sigma factor B (σB). These m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00751-19
更新日期:2020-04-09 00:00:00
abstract::The DNA binding proteins ArgR and AhrC are essential for regulation of arginine metabolism in Escherichia coli and Bacillus subtilis, respectively. A unique property of these regulators is that they form hexameric protein complexes, mediating repression of arginine biosynthetic pathways as well as activation of argini...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1147-1157.2004
更新日期:2004-02-01 00:00:00
abstract::Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intrace...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01997-07
更新日期:2008-04-01 00:00:00
abstract::The ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.474-480.1972
更新日期:1972-08-01 00:00:00
abstract::Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.8.1989-1993.1995
更新日期:1995-04-01 00:00:00
abstract::The Sinorhizobium meliloti megaplasmid pSymA has previously been implicated in gluconate utilization. We report a locus on pSymA encoding a putative tripartite ATP-independent periplasmic (TRAP) transporter that is required for gluconate utilization. The expression of this locus is negatively regulated by a GntR famil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01317-08
更新日期:2009-02-01 00:00:00
abstract::Sneathia amnii is a poorly characterized emerging pathogen that has been implicated in amnionitis and urethritis. We found that S. amnii damages fetal membranes, and we identified and purified a cytotoxic exotoxin that lyses human red blood cells and damages cells from fetal membranes. The gene appears to be cotranscr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00162-20
更新日期:2020-06-09 00:00:00