Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha.

abstract：:A fragment of the Lactococcus lactis chromosome containing an open reading frame of 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family of regulatory proteins including ArbG from Erwinia chrysanthemi, BglG from Escherichia coli, and SacT an...

journal_title：Journal of bacteriology

authors： Bardowski J,Ehrlich SD,Chopin A

更新日期：1994-09-01 00:00:00

abstract：:Analysis of chlorobenzene-degrading transconjugants of Pseudomonas putida F1 which had acquired the genes for chlorocatechol degradation (clc) from Pseudomonas sp. strain B13 revealed that the clc gene cluster was present on a 105-kb amplifiable genetic element (named the clc element). In one such transconjugant, P. p...

journal_title：Journal of bacteriology

authors： Ravatn R,Studer S,Springael D,Zehnder AJ,van der Meer JR

更新日期：1998-09-01 00:00:00

abstract：:Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...

journal_title：Journal of bacteriology

authors： Gromkova RH

更新日期：1971-06-01 00:00:00

abstract：:The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

journal_title：Journal of bacteriology

authors： Griffiths MW,Sundaram TK

更新日期：1973-12-01 00:00:00

abstract：:Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a approximately 90% reduction in alginat...

journal_title：Journal of bacteriology

authors： Silo-Suh L,Suh SJ,Phibbs PV,Ohman DE

更新日期：2005-11-01 00:00:00

abstract：:We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...

journal_title：Journal of bacteriology

authors： Katayama Y,Takeuchi F,Ito T,Ma XX,Ui-Mizutani Y,Kobayashi I,Hiramatsu K

更新日期：2003-05-01 00:00:00

abstract：:Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoin to urea. As a result, these activities increase only 4- to 8-fold upon induction, whereas the urea-degrading enzymes, urea carboxylase a...

journal_title：Journal of bacteriology

authors： Zacharski CA,Cooper TG

更新日期：1978-08-01 00:00:00

abstract：:Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

journal_title：Journal of bacteriology

authors： Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

更新日期：1997-01-01 00:00:00

abstract：:The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...

journal_title：Journal of bacteriology

authors： Kasahara M,Makino K,Amemura M,Nakata A,Shinagawa H

更新日期：1991-01-01 00:00:00

abstract：:Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

journal_title：Journal of bacteriology

authors： Tisa LS,Ensign JC

更新日期：1988-07-01 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsatu...

journal_title：Journal of bacteriology

authors： French CE,Nicklin S,Bruce NC

更新日期：1996-11-01 00:00:00

abstract：:Two-component signal transduction systems are utilized by prokaryotic and eukaryotic cells to sense and respond to environmental stimuli, both to maintain homeostasis and to rapidly adapt to changing conditions. Studies have begun to emerge that utilize a large-scale mutagenesis approach to analyzing these systems in ...

journal_title：Journal of bacteriology

authors： Hussa EA,O'Shea TM,Darnell CL,Ruby EG,Visick KL

更新日期：2007-08-01 00:00:00

abstract：:A study of the distribution of pyrrolidone carboxylyl peptidase (PCP) activity among cell fractions of Streptococcus cremoris HP revealed that this enzyme is associated with a particulate fraction, which mainly consists of membrane material. This location could only be established using a gentle nonmechanical method f...

journal_title：Journal of bacteriology

authors： Exterkate FA

更新日期：1977-03-01 00:00:00

abstract：:Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

journal_title：Journal of bacteriology

authors： Garcia DL,Dillard JP

更新日期：2008-06-01 00:00:00

abstract：:Surgalla, M. J. (Fort Detrick, Frederick, Md.), A. W. Andrews, and C. L. Baugh. Effects of bicarbonate on growth of Pasteurella pestis. I. Differential response of virulent and avirulent cells. J. Bacteriol. 88:269-272. 1964.-Virulent Pasteurella pestis and the avirulent mutants always present in a virulent inoculum h...

journal_title：Journal of bacteriology

authors： SURGALLA MJ,ANDREWS AW,BAUGH CL

更新日期：1964-08-01 00:00:00

abstract：:The hpaB gene encoding an aromatic hydroxylase of Escherichia coli ATCC 11105, a penicillin G acylase-producing strain, has been cloned and expressed in E. coli K-12. This gene was located near the pacA gene coding for penicillin G acylase. The hydroxylase has a molecular mass of 59,000 Da, uses NADH as a cosubstrate,...

journal_title：Journal of bacteriology

authors： Prieto MA,Perez-Aranda A,Garcia JL

更新日期：1993-04-01 00:00:00

abstract：:A phage-induced cell wall solubilizing enzyme isolated from phage-infected Staphylococcus aureus phage type 80 was purified 588-fold. The pH optimal activity was 6.8 to 7.3, and pH optimal stability, 6.5 to 7.5. It was inhibited by p-hydroxymercuribenzoate, ethylenediaminetetraacetic acid, and specific rabbit antisera...

journal_title：Journal of bacteriology

authors： Doughty CC,Mann JA

更新日期：1967-03-01 00:00:00

abstract：:Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...

journal_title：Journal of bacteriology

authors： Lei MG,Cue D,Roux CM,Dunman PM,Lee CY

更新日期：2011-10-01 00:00:00

abstract：:A temperate phage, phi CTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of phi CTX-resistant mutants derived from phi CTX-sensitive strains. phi CTX infectivity was neutralized by LPS preparations derived from sensitive strains but ...

journal_title：Journal of bacteriology

authors： Yokota S,Hayashi T,Matsumoto H

更新日期：1994-09-01 00:00:00

abstract：:Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...

journal_title：Journal of bacteriology

authors： Shaw JH,Clewell DB

更新日期：1985-11-01 00:00:00

abstract：:A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

journal_title：Journal of bacteriology

authors： Donadio S,Shafiee A,Hutchinson CR

更新日期：1990-01-01 00:00:00

abstract：:Previous studies have indicated that a single enzyme, "kynureninase," catalyzes the reactions of l-kynurenine to anthranilate and l-3-hydroxykynurenine to 3-hydroxyanthranilate in Neurospora crassa and in other organisms. The present report describes separate enzymes which catalyze these reactions in N. crassa. The fi...

journal_title：Journal of bacteriology

authors： Gaertner FH,Cole KW,Welch GR

更新日期：1971-11-01 00:00:00

abstract：:Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been i...

journal_title：Journal of bacteriology

authors： Aoyama K,Mitsubayashi Y,Aiba H,Mizuno T

更新日期：2000-09-01 00:00:00

abstract：:The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially composed of N-acetylglycosaminyl-N-acetylmuramic acid disaccharide units. Small amounts of N-acetylglycosaminyl-N-glyc...

journal_title：Journal of bacteriology

authors： Janczura E,Leyh-Bouille M,Cocito C,Ghuysen JM

更新日期：1981-02-01 00:00:00

abstract：:Carbon catabolite repression (CCR) allows bacteria to alter metabolism in response to the availability of specific sugar sources, and increasing evidence suggests that CCR is involved in regulating virulence gene expression in many pathogens. A scan of the M1 SF370 group A streptococcus (GAS) genome using a Bacillus s...

journal_title：Journal of bacteriology

authors： Almengor AC,Kinkel TL,Day SJ,McIver KS

更新日期：2007-12-01 00:00:00

abstract：:Two nifA-like genes, designated anfA and vnfA, have been identified in Azotobacter vinelandii. The anfA gene is located upstream from the nitrogenase-3 structural gene cluster (anfHDGK) and is preceded by a sequence that is potentially part of a ntrA-dependent promoter. The product of anfA appears to be required for e...

journal_title：Journal of bacteriology

authors： Joerger RD,Jacobson MR,Bishop PE

更新日期：1989-06-01 00:00:00

abstract：:We have developed a cytoduction assay to measure cell fusion quantitatively in the basidiomycete corn smut fungus Ustilago maydis. This assay employs a mutation conferring resistance to oligomycin that exhibits non-Mendelian inheritance and presumably affects the mitochondrial genome. After auxotrophic olir cells are ...

journal_title：Journal of bacteriology

authors： Trueheart J,Herskowitz I

更新日期：1992-12-01 00:00:00

abstract：:Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA. ...

journal_title：Journal of bacteriology

authors： Sancar A,Hack AM,Rupp WD

更新日期：1979-01-01 00:00:00

abstract：:The cis-acting regulatory regions of the tps and ops genes of Myxococcus xanthus were localized by analyzing the expression of fusions of these genes with the lacZ gene. A 201-base-pair (bp) fragment of tps DNa extending 95 bp upstream (-95) from the transcriptional start was sufficient to direct developmentally regul...

journal_title：Journal of bacteriology

authors： Downard JS,Kim SH,Kil KS

更新日期：1988-10-01 00:00:00