Isocitrate lyase from a thermophilic Bacillus: effect of salts on enzyme activity.

abstract：:The highly toxic oxyanion tellurite (TeO3(2-)) is a well known pro-oxidant in mammalian and bacterial cells. This work examines the effects of tellurite on the redox state of the electron transport chain of the facultative phototroph Rhodobacter capsulatus, in relation to the role of the thiol:disulfide oxidoreductase...

journal_title：Journal of bacteriology

authors： Borsetti F,Francia F,Turner RJ,Zannoni D

更新日期：2007-02-01 00:00:00

abstract：:Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

journal_title：Journal of bacteriology

authors： Rawls KS,Martin JH,Maupin-Furlow JA

更新日期：2011-09-01 00:00:00

abstract：:Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...

journal_title：Journal of bacteriology

authors： van Solingen P,van der Plaat JB

更新日期：1977-05-01 00:00:00

abstract：:Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...

journal_title：Journal of bacteriology

authors： Snow R

更新日期：1967-09-01 00:00:00

abstract：:The virulence of Pseudomonas aeruginosa and other surface pathogens involves the coordinate expression of a wide range of virulence determinants, including type IV pili. These surface filaments are important for the colonization of host epithelial tissues and mediate bacterial attachment to, and translocation across, ...

journal_title：Journal of bacteriology

authors： Leech AJ,Mattick JS

更新日期：2006-12-01 00:00:00

abstract：:Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...

journal_title：Journal of bacteriology

authors： Sánchez L,Pan W,Viñas M,Nikaido H

更新日期：1997-11-01 00:00:00

abstract：:Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 7...

journal_title：Journal of bacteriology

authors： Verma A,Cheung AM,Burns DL

更新日期：2008-11-01 00:00:00

abstract：:Phytoplasmas are plant-pathogenic bacteria that cause numerous diseases. This study shows a strong positive selection on the phytoplasma antigenic membrane protein (Amp). The ratio of nonsynonymous to synonymous substitutions was >1 with all the methods we tested. The clear positive selections imply an important biolo...

journal_title：Journal of bacteriology

authors： Kakizawa S,Oshima K,Jung HY,Suzuki S,Nishigawa H,Arashida R,Miyata S,Ugaki M,Kishino H,Namba S

更新日期：2006-05-01 00:00:00

abstract：:A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

journal_title：Journal of bacteriology

authors： Kelly R,Kwon-Chung KJ

更新日期：1992-01-01 00:00:00

abstract：:The salmonellae are a diverse group of bacteria within the family Enterobacteriaceae that includes two species, Salmonella enterica and Salmonella bongori. In order to characterize the phylogenetic relationships of the species and subspecies of Salmonella, we analyzed four housekeeping genes, gapA, phoP, mdh and recA,...

journal_title：Journal of bacteriology

authors： McQuiston JR,Herrera-Leon S,Wertheim BC,Doyle J,Fields PI,Tauxe RV,Logsdon JM Jr

更新日期：2008-11-01 00:00:00

abstract：:A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

journal_title：Journal of bacteriology

authors： Gay NJ

更新日期：1984-06-01 00:00:00

abstract：:Diazouracil (DZU) was shown to increase the mean cell volume of several bacterial species. The filaments which it induced with Escherichia coli B and Lactobacillus casei were examined in section by electron microscopy. In addition to the inhibition of division of E. coli by DZU, its effects were studied on mass increa...

journal_title：Journal of bacteriology

authors： Previc E,Richardson S

更新日期：1969-01-01 00:00:00

abstract：:Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. a...

journal_title：Journal of bacteriology

authors： Jensen RA,Twarog R

更新日期：1972-09-01 00:00:00

abstract：:Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...

journal_title：Journal of bacteriology

authors： Kuhla J,Oelze J

更新日期：1988-11-01 00:00:00

abstract：:A method was devised to efficiently select isolates of Staphylococcus aureus 8325 in which Tn551, a transposon originating on the pI258 plasmid responsible for erythromycin resistance (Emr), had translocated to the host chromosome. This method consisted of selecting for Emr at 43 degrees C with a strain in which the p...

journal_title：Journal of bacteriology

authors： Pattee PA

更新日期：1981-01-01 00:00:00

abstract：:The ToxR protein of Vibrio cholerae regulates the expression of several virulence factors that play important roles in the pathogenesis of cholera. Previous experiments with ToxR-alkaline phosphatase (ToxR-PhoA) fusion proteins suggested a model for gene regulation in which the inactive form of ToxR was a monomer and ...

journal_title：Journal of bacteriology

authors： Ottemann KM,Mekalanos JJ

更新日期：1996-01-01 00:00:00

abstract：:Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...

journal_title：Journal of bacteriology

authors： Aronson AI,Bell C,Fulroth B

更新日期：2005-05-01 00:00:00

abstract：:A partial nucleotide sequence that included 1,693 base pairs of the M12 (emm12) gene of group A streptococci (strain CS24) and adjacent upstream DNA was determined. Type 12 M protein-specific mRNA of strain CS24 is transcribed from two promoters (P1 and P3) separated by 30 bases. The transcription start sites of the e...

journal_title：Journal of bacteriology

authors： Robbins JC,Spanier JG,Jones SJ,Simpson WJ,Cleary PP

更新日期：1987-12-01 00:00:00

abstract：:DifA is a methyl-accepting chemotaxis protein (MCP)-like sensory transducer that regulates exopolysaccharide (EPS) production in Myxococcus xanthus. Here mutational analysis and molecular biology were used to probe the signaling mechanisms of DifA in EPS regulation. We first identified the start codon of DifA experime...

journal_title：Journal of bacteriology

authors： Xu Q,Black WP,Nascimi HM,Yang Z

更新日期：2011-02-01 00:00:00

abstract：:Whiteley, H. R. (University of Washington, Seattle) and N. G. McCormick. Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction. J. Bacteriol. 85:382-393. 1963.-At an acid pH, extracts of Micrococcus lactilyticus (Veillonella alcalescens) ...

journal_title：Journal of bacteriology

authors： WHITELEY HR,McCORMICK NG

更新日期：1963-02-01 00:00:00

abstract：:NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate con...

journal_title：Journal of bacteriology

authors： Goffin P,Lorquet F,Kleerebezem M,Hols P

更新日期：2004-10-01 00:00:00

abstract：:The bacterial second messenger cyclic di-GMP (c-di-GMP) regulates the transition between sessility and motility. In Salmonella enterica serovar Typhimurium, the expression of CsgD, the regulator of multicellular rdar morphotype behavior, is a major target of c-di-GMP signaling. CsgD expression is positively regulated ...

journal_title：Journal of bacteriology

authors： Simm R,Remminghorst U,Ahmad I,Zakikhany K,Römling U

更新日期：2009-06-01 00:00:00

abstract：:We have investigated the mechanism of osmotic stress adaptation (osmoregulation) in Agrobacterium tumefaciens biotype I (salt-tolerant) and biotype II (salt-sensitive) strains. Using natural-abundance 13C nuclear magnetic resonance spectroscopy, we identified all organic solutes that accumulated to significant levels ...

journal_title：Journal of bacteriology

authors： Smith LT,Smith GM,Madkour MA

更新日期：1990-12-01 00:00:00

abstract：:ZoBell, Claude E. (University of California, La Jolla) and Andre B. Cobet. Growth, reproduction, and death rates of Escherichia coli at increased hydrostatic pressures. J. Bacteriol. 84:1228-1236. 1962.-Pressures ranging from 100 to 500 atm were found to retard the growth and reproduction of Escherichia coli in nutrie...

journal_title：Journal of bacteriology

authors： ZOBELL CE,COBET AB

更新日期：1962-12-01 00:00:00

abstract：:Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH), a halogenated organic insecticide, as a sole carbon and energy source. In a previous study, we showed that gamma-HCH is degraded to 2,5-dichlorohydroquinone (2,5-DCHQ) (Y. Nagata, R. Ohtomo, K. Miyauchi, M. Fukuda, ...

journal_title：Journal of bacteriology

authors： Miyauchi K,Suh SK,Nagata Y,Takagi M

更新日期：1998-03-01 00:00:00

abstract：:Five clostridial species were found to ferment gluconate via 2-keto-3-deoxygluconate which subsequently is phosphorylated to yield 2-keto-3-deoxy-6-phosphogluconate (KDPG). This compound is then cleaved by KDPG aldolase. ...

journal_title：Journal of bacteriology

authors： Bender R,Andreesen JR,Gottschalk G

更新日期：1971-08-01 00:00:00

abstract：:Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short temperature-sensitive period that occurs at about the time when the spore septum is formed. Cells can escape the sporulation block, if incubated for a short period at the permissive temperature,...

journal_title：Journal of bacteriology

authors： Young M

更新日期：1976-05-01 00:00:00

abstract：:Following arrest by UV-induced DNA damage, replication is restored through a sequence of steps that involve partial resection of the nascent DNA by RecJ and RecQ, branch migration and processing of the fork DNA surrounding the lesion by RecA and RecF-O-R, and resumption of DNA synthesis once the blocking lesion has be...

journal_title：Journal of bacteriology

authors： Courcelle CT,Landstrom AJ,Anderson B,Courcelle J

更新日期：2012-08-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Myobacterium tuberculosis. J. Bacteriol. 91:2146-2154. 1966.-The ribosomal fraction of the attenuated strain, H37R...

journal_title：Journal of bacteriology

authors： Youmans AS,Youmans GP

更新日期：1966-06-01 00:00:00

abstract：:Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium and a common commensal organism of the upper respiratory tract in humans. NTHi causes a number of diseases, including otitis media, sinusitis, conjunctivitis, exacerbations of chronic obstructive pulmonary disease, and bronchitis. During the course...

journal_title：Journal of bacteriology

authors： Harrison A,Ray WC,Baker BD,Armbruster DW,Bakaletz LO,Munson RS Jr

更新日期：2007-02-01 00:00:00