Cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane by Sphingomonas paucimobilis.

abstract：:Desulfomonile tiedjei DCB-1, a sulfate-reducing bacterium, conserves energy for growth from reductive dehalogenation of 3-chlorobenzoate by an uncharacterized chemiosmotic process. Respiratory electron transport components were examined in D. tiedjei cells grown under conditions for reductive dehalogenation, pyruvate ...

journal_title：Journal of bacteriology

authors： Louie TM,Mohn WW

更新日期：1999-01-01 00:00:00

abstract：:We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in bo...

journal_title：Journal of bacteriology

authors： Donnelly CE,Sonenshein AL

更新日期：1984-03-01 00:00:00

abstract：:It has been previously established that Thiobacillus neapolitanus fixes CO2 by using a form I ribulose bisphosphate carboxylase/oxygenase (RuBisCO), that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL and cbbS, are part of a putative carboxysome operon. In the present stud...

journal_title：Journal of bacteriology

authors： Baker SH,Jin S,Aldrich HC,Howard GT,Shively JM

更新日期：1998-08-01 00:00:00

abstract：:In Caulobacter crescentus biogenesis of the flagellar organelle occurs during one stage of its complex life cycle. Thus in synchronous cultures it is possible to assay the sequential synthesis and assembly of the flagellum and hook in vivo with a combination of biochemical and radioimmunological techniques. The period...

journal_title：Journal of bacteriology

authors： Lagenaur C,Agabian N

更新日期：1978-09-01 00:00:00

abstract：:We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...

journal_title：Journal of bacteriology

authors： Mekalanos JJ,Sublett RD,Romig WR

更新日期：1979-09-01 00:00:00

abstract：:Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...

journal_title：Journal of bacteriology

authors： Hou CT,Lillard MO

更新日期：1976-04-01 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...

journal_title：Journal of bacteriology

authors： Morohoshi T,Nakamura Y,Yamazaki G,Ishida A,Kato N,Ikeda T

更新日期：2007-11-01 00:00:00

abstract：:E2 (dihydrolipoamide acetyltransferase) and E3 (dihydrolipoamide dehydrogenase) of the Clostridium magnum acetoin dehydrogenase enzyme system were copurified in a three-step procedure from acetoin-grown cells. The denatured E2-E3 preparation comprised two polypeptides with M(r)s of 49,000 and 67,000, respectively. Mic...

journal_title：Journal of bacteriology

authors： Krüger N,Oppermann FB,Lorenzl H,Steinbüchel A

更新日期：1994-06-01 00:00:00

abstract：:The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...

journal_title：Journal of bacteriology

authors： Lynch WH

更新日期：1980-07-01 00:00:00

abstract：:Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a pr...

journal_title：Journal of bacteriology

authors： Boucher DJ,Adler B,Boyce JD

更新日期：2005-04-01 00:00:00

abstract：:The kinetics of chromosome replication and cell division have been examined in recA mutants of Escherichia coli B/r containing F' plasmids of various sizes. Plasmid-mediated alterations in growth properties were detected only with the presence of the larger F' plasmids, and were reflected in decreased mean cell sizes ...

journal_title：Journal of bacteriology

authors： Weinberger M,Helmstetter CE

更新日期：1979-03-01 00:00:00

abstract：:By using the isotope pairs (22)Na-(24)Na and (42)K-(86)Rb, the uptake and retention of Na and K was studied in the salt-tolerant Debaryomyces hansenii and in the less tolerant Saccharomyces cerevisiae at NaCl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. The ratio of K to Na is much higher in the cells than ...

journal_title：Journal of bacteriology

authors： Norkrans B,Kylin A

更新日期：1969-11-01 00:00:00

abstract：:No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

journal_title：Journal of bacteriology

authors： Low IE,Eaton MD,Proctor P

更新日期：1968-04-01 00:00:00

abstract：:Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...

journal_title：Journal of bacteriology

authors： Tokunaga T,Rashid MH,Kuroda A,Sekiguchi J

更新日期：1994-08-01 00:00:00

abstract：:Hertman, I. (Israel Institute for Biological Research, Ness Ziona, Israel). Bacteriophage common to Pasteurella pestis and Escherichia coli. J. Bacteriol. 88:1002-1005. 1964.-Phage Y of Pasteurella pestis and phage T(3) of Escherichia coli are serologically related and have common bacterial hosts. Anti-Y serum neutral...

journal_title：Journal of bacteriology

authors： HERTMAN I

更新日期：1964-10-01 00:00:00

abstract：:The bacteriophage Mu genome contains a centrally located strong gyrase site (SGS) that is required for efficient prophage replication. To aid in studying the unusual properties of the SGS, we sought other gyrase sites that might be able to substitute for the SGS in Mu replication. Five candidate sites were obtained by...

journal_title：Journal of bacteriology

authors： Oram M,Pato ML

更新日期：2004-07-01 00:00:00

abstract：:The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...

journal_title：Journal of bacteriology

authors： Miller P,Mueller J,Hill K,Taber H

更新日期：1988-06-01 00:00:00

abstract：:The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...

journal_title：Journal of bacteriology

authors： Götz R,Schmitt R

更新日期：1987-07-01 00:00:00

abstract：:Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97-103. 1964.-A factor present in plant and animal sources was found necessary for the incorporation of formate-C(14) into pyr...

journal_title：Journal of bacteriology

authors： WOOD NP,O'KANE DJ

更新日期：1964-01-01 00:00:00

abstract：:The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times...

journal_title：Journal of bacteriology

authors： Willison JC,Tissot G

更新日期：1994-06-01 00:00:00

abstract：:The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. An rpoS mutant was examined under steady-state conditions with acetate as an electron donor and fumarate as an electron acceptor and with additional transcriptional ...

journal_title：Journal of bacteriology

authors： Núñez C,Esteve-Núñez A,Giometti C,Tollaksen S,Khare T,Lin W,Lovley DR,Methé BA

更新日期：2006-04-01 00:00:00

abstract：:The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies with F-pilin polypeptides in inner membrane preparations from various F mutant strains. It was known that JEL92 recognizes ...

journal_title：Journal of bacteriology

authors： Moore D,Hamilton CM,Maneewannakul K,Mintz Y,Frost LS,Ippen-Ihler K

更新日期：1993-03-01 00:00:00

abstract：:Enzymes of the ATP-independent Deg serine endopeptidase family are very flexible with regard to their substrate specificity. Some family members cleave only one substrate, while others act as general proteases on unfolded substrates. The proteolytic activity of Deg proteases is regulated by PDZ protein interaction dom...

journal_title：Journal of bacteriology

authors： Huesgen PF,Scholz P,Adamska I

更新日期：2007-09-01 00:00:00

abstract：:Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...

journal_title：Journal of bacteriology

authors： Bonato MC,da Costa Maia JC,Juliani MH

更新日期：1983-06-01 00:00:00

abstract：:Spectinomycin-resistant (Spcr) mutants of Escherichia coli were isolated from nutrient agar plates containing 20% sucrose and 100 mug of spectinomycin per ml. About one-third of the Spcr mutants thus obtained were sucrose dependent (Sucd) and were classified into two types: I, those unable to grow on sucrose-free medi...

journal_title：Journal of bacteriology

authors： Miyoshi Y,Yamagata H

更新日期：1976-01-01 00:00:00

abstract：:The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

journal_title：Journal of bacteriology

authors： Smith WR,Yu I,Hungate RE

更新日期：1973-05-01 00:00:00

abstract：:The effects of the free bile acids (FBAs) cholic acid (CA), deoxycholic acid (DCA), and chenodeoxycholic acid on the bioenergetics and growth of lactobacilli and bifidobacteria were investigated. It was found that these FBAs reduced the internal pH levels of these bacteria with rapid and stepwise kinetics and, at cert...

journal_title：Journal of bacteriology

authors： Kurdi P,Kawanishi K,Mizutani K,Yokota A

更新日期：2006-03-01 00:00:00

abstract：:Tabak, Henry H. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Cecil W. Chambers, and Paul W. Kabler. Microbial metabolism of aromatic carbon compounds. I. Decomposition of phenolic compounds and aromatic hydrocarbons by phenol-adapted bacteria. J. Bacteriol. 87:910-919. 1964.-Bacteria from soil and r...

journal_title：Journal of bacteriology

authors： TABAK HH,CHAMBERS CW,KABLER PW

更新日期：1964-04-01 00:00:00

abstract：:Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...

journal_title：Journal of bacteriology

authors： Crowe BA,Owen P

更新日期：1983-03-01 00:00:00