Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.

abstract：:The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...

journal_title：Journal of bacteriology

authors： Ishihama A,Kori A,Koshio E,Yamada K,Maeda H,Shimada T,Makinoshima H,Iwata A,Fujita N

更新日期：2014-08-01 00:00:00

abstract：:The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...

journal_title：Journal of bacteriology

authors： Inatomi K,Maeda M

更新日期：1988-12-01 00:00:00

abstract：:The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...

journal_title：Journal of bacteriology

authors： Dhiman A,Schleif R

更新日期：2000-09-01 00:00:00

abstract：:Wild-type strains of Escherichia coli K-12 are resistant to acriflavine. Gene acrA(+) which determines resistance to acriflavine is located near the lac region of the chromosome. This gene determines not only resistance to basic dyes but also resistance to phenethyl alcohol. Acriflavine resistance was transmitted, tog...

journal_title：Journal of bacteriology

authors： Nakamura H

更新日期：1968-10-01 00:00:00

abstract：:SpoVM is an unusually small (26-residue-long) protein that is produced in the mother cell chamber of the sporangium during the process of sporulation in Bacillus subtilis. We investigated the subcellular localization of SpoVM, which is believed to be an amphipathic alpha-helix, by using a fusion of the sporulation pro...

journal_title：Journal of bacteriology

authors： van Ooij C,Losick R

更新日期：2003-02-01 00:00:00

abstract：:The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...

journal_title：Journal of bacteriology

authors： Austin SJ

更新日期：1984-05-01 00:00:00

abstract：:A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...

journal_title：Journal of bacteriology

authors： Brandner JP,McEwan AG,Kaplan S,Donohue TJ

更新日期：1989-01-01 00:00:00

abstract：:Strains of Lactobacillus casei subsp. rhamnosus possessing two cell wall polysaccharides, a hexosamine-containing H-polysaccharide and a rhamnose-containing R-polysaccharide, were examined for the effect of growth conditions on the production of these two components. In strain NCTC 6375, R- and H-polysaccharides accou...

journal_title：Journal of bacteriology

authors： Wicken AJ,Ayres A,Campbell LK,Knox KW

更新日期：1983-01-01 00:00:00

abstract：:Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...

journal_title：Journal of bacteriology

authors： Aronson JN,Wermus GR

更新日期：1965-07-01 00:00:00

abstract：:Upon induction, Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills B. megaterium and a few other bacterial species. The DNA region responsible for megacinogeny was cloned in B. megaterium. The nucleotide sequence of a 5,494-bp-long subfragment was determ...

journal_title：Journal of bacteriology

authors： Kiss A,Balikó G,Csorba A,Chuluunbaatar T,Medzihradszky KF,Alföldi L

更新日期：2008-10-01 00:00:00

abstract：:Fundamental questions in bacterial gene regulation concern how multiple regulatory proteins interact with the transcription apparatus at a single promoter and what are the roles of protein contacts with RNA polymerase and changes in DNA conformation. Transcription of the Escherichia coli uhpT gene, encoding the induci...

journal_title：Journal of bacteriology

authors： Olekhnovich IN,Kadner RJ

更新日期：1999-12-01 00:00:00

abstract：:The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...

journal_title：Journal of bacteriology

authors： Nakahara H,Silver S,Miki T,Rownd RH

更新日期：1979-10-01 00:00:00

abstract：:The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...

journal_title：Journal of bacteriology

authors： Stibitz S,Weiss AA,Falkow S

更新日期：1988-07-01 00:00:00

abstract：:Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...

journal_title：Journal of bacteriology

authors： Straney R,Krah R,Menzel R

更新日期：1994-10-01 00:00:00

abstract：:The polar pili of Pseudomonas aeruginosa are composed of subunits encoded by the pilA gene. Expression of pilA requires the alternative sigma factor RpoN and a pair of regulatory elements, PilS and PilR. These two proteins are members of the two-component regulatory family, in which PilS is the sensory component and P...

journal_title：Journal of bacteriology

authors： Boyd JM,Lory S

更新日期：1996-02-01 00:00:00

abstract：:In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane p...

journal_title：Journal of bacteriology

authors： Yurist S,Dahan I,Eichler J

更新日期：2007-01-01 00:00:00

abstract：:Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utiliz...

journal_title：Journal of bacteriology

authors： Lippincott JA,Beiderbeck R,Lippincott BB

更新日期：1973-10-01 00:00:00

abstract：:The first enzyme in the biosynthesis of leucine in yeast, alpha-isopropylmalate synthetase, is inhibited by l-leucine. In a mutant resistant to the analogue 5',5',5'-trifluoroleucine, the enzyme is markedly resistant to inhibition by l-leucine. Growth ing the presence of exogenous l-leucine results in repression of th...

journal_title：Journal of bacteriology

authors： Satyanarayana T,Umbarger HE,Lindegren G

更新日期：1968-12-01 00:00:00

abstract：:Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...

journal_title：Journal of bacteriology

authors： Kjellin-Stråby K

更新日期：1969-11-01 00:00:00

abstract：:The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cle...

journal_title：Journal of bacteriology

authors： Hoa NT,Brannigan JA,Cutting SM

更新日期：2002-01-01 00:00:00

abstract：:The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

journal_title：Journal of bacteriology

authors： Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

更新日期：1990-05-01 00:00:00

abstract：:Pseudomonas aeruginosa and many other bacteria can utilize biogenic polyamines, including diaminopropane (DAP), putrescine (Put), cadaverine (Cad), and spermidine (Spd), as carbon and/or nitrogen sources. Transcriptome analysis in response to exogenous Put and Spd led to the identification of a list of genes encoding ...

journal_title：Journal of bacteriology

authors： Yao X,He W,Lu CD

更新日期：2011-08-01 00:00:00

abstract：:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...

journal_title：Journal of bacteriology

authors： Gyohda A,Funayama N,Komano T

更新日期：1997-03-01 00:00:00

abstract：:The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...

journal_title：Journal of bacteriology

authors： Slack FJ,Mueller JP,Sonenshein AL

更新日期：1993-08-01 00:00:00

abstract：:Structural analogs of S-adenosylhomocysteine were tested in vitro for inhibition of the yeast S-adenosylmethionine:delta 24-sterol-C-methyltransferase enzyme. A wide inhibitory range by these compounds was observed, suggesting which structural features of the parent compound are important for binding to the enzyme. No...

journal_title：Journal of bacteriology

authors： McCammon MT,Parks LW

更新日期：1981-01-01 00:00:00

abstract：:We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) and fastidious, obligately microaerophilic species, such as Magnetospirillum magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from strains MGT-1 and AMB-1 was used as ...

journal_title：Journal of bacteriology

authors： Burgess JG,Kawaguchi R,Sakaguchi T,Thornhill RH,Matsunaga T

更新日期：1993-10-01 00:00:00

abstract：:MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mo...

journal_title：Journal of bacteriology

authors： Perwez T,Meyer RJ

更新日期：1999-04-01 00:00:00

abstract：:The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...

journal_title：Journal of bacteriology

authors： Loroch AI,Nguyen BG,Ludwig RA

更新日期：1995-12-01 00:00:00

abstract：:It is thought that in the gram-negative soil bacterium Sinorhizobium meliloti the protein ExoP is involved in biosynthesis of the acidic exopolysaccharide succinoglycan (EPS I). The amounts and compositions of EPS I produced by mutants expressing ExoP proteins characterized by specific amino acid substitutions in the ...

journal_title：Journal of bacteriology

authors： Niemeyer D,Becker A

更新日期：2001-09-01 00:00:00

abstract：:The tail structures of bacteriophages infecting gram-positive bacteria are largely unexplored, although the phage tail mediates the initial interaction with the host cell. The temperate Lactococcus lactis phage TP901-1 of the Siphoviridae family has a long noncontractile tail with a distal baseplate. In the present st...

journal_title：Journal of bacteriology

authors： Vegge CS,Brøndsted L,Neve H,Mc Grath S,van Sinderen D,Vogensen FK

更新日期：2005-06-01 00:00:00