Hypersensitivity to Hg2+ and hyperbinding activity associated with cloned fragments of the mercurial resistance operon of plasmid NR1.

abstract：:Transition metals are essential for life but are toxic when in excess. Metal ion intoxication may result from the mismetallation of essential metal-dependent enzymes with a noncognate metal. To begin to identify enzymes and processes that are susceptible to mismetallation, we have selected for strains with increased r...

journal_title：Journal of bacteriology

authors： Pi H,Wendel BM,Helmann JD

更新日期：2020-03-11 00:00:00

abstract：:The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...

journal_title：Journal of bacteriology

authors： Ma K,Weiss R,Adams MW

更新日期：2000-04-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...

journal_title：Journal of bacteriology

authors： Thanedar S,Dineshkumar TK,Varshney U

更新日期：2001-12-01 00:00:00

abstract：:Jones, Lois M. (University of Wisconsin, Madison), C. R. McDuff, and J. B. Wilson. Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state. J. Bacteriol. 83:860-866. 1962.-In the course of examining a number of Brucella cultures with a brucellaphage, it was observed t...

journal_title：Journal of bacteriology

authors： JONES LM,McDUFF CR,WILSON JB

更新日期：1962-04-01 00:00:00

abstract：:The ASM Conference on Mechanisms of Interbacterial Cooperation and Competition was held in Washington DC, from 1 to 4 March 2017. The conference provided an international forum for sociomicrobiologists from different disciplines to present and discuss new findings. The meeting covered a wide range of topics, spanning ...

journal_title：Journal of bacteriology

authors： Lories B,Parijs I,Foster KR,Steenackers HP

更新日期：2017-08-14 00:00:00

abstract：:Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in in UvrB strains containing both plasmid pNP5 and...

journal_title：Journal of bacteriology

authors： Pannekoek H,Noordermeer I,van de Putte P

更新日期：1979-07-01 00:00:00

abstract：:Ruminococcin A (RumA) is a trypsin-dependent lantibiotic produced by Ruminococcus gnavus E1, a gram-positive strict anaerobic strain isolated from a human intestinal microbiota. A 12.8-kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumA, has been cloned and sequenced. It consisted ...

journal_title：Journal of bacteriology

authors： Gomez A,Ladiré M,Marcille F,Fons M

更新日期：2002-01-01 00:00:00

abstract：:A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...

journal_title：Journal of bacteriology

authors： Alber BE,Kung JW,Fuchs G

更新日期：2008-02-01 00:00:00

abstract：:Mutants of Escherichia coli K-12 constitutive for fatty acid degradation (fadR) showed an increased rate of utilization of exogenous acetate. Acetate transport, oxidation, and incorporation into macromolecules was approximately fivefold greater in fadR mutants than fadR+ strains during growth on succinate as a carbon ...

journal_title：Journal of bacteriology

authors： Maloy SR,Nunn WD

更新日期：1981-10-01 00:00:00

abstract：:A procedure is described that uses an indicator plasmid (pSC201) to identify cells in a bacterial population that have been co-transformed with a second plasmid lacking detectable phenotypic properties. Under appropriate conditions of indirect selection, between 50 and 85% of transformants carrying the indicator plasm...

journal_title：Journal of bacteriology

authors： Kretschmer FJ,Chang AC,Cohen SN

更新日期：1975-10-01 00:00:00

abstract：UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...

journal_title：Journal of bacteriology

authors： Pal Chowdhury P,Basu S,Dutta A,Dutta TK

更新日期：2016-05-27 00:00:00

abstract：:Bacillus subtilis cells cannot sporulate in the presence of catabolites such as glucose. During the analysis of Tn10-generated mutants, we found that deletion of the C-terminal region of the tnrA gene, which encodes a global regulator that positively regulates a number of genes in response to nitrogen limitation, resu...

journal_title：Journal of bacteriology

authors： Shin BS,Choi SK,Smith I,Park SH

更新日期：2000-09-01 00:00:00

abstract：:The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...

journal_title：Journal of bacteriology

authors： Chatfield LK,Orr E,Boulnois GJ,Wilkins BM

更新日期：1982-12-01 00:00:00

abstract：:A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...

journal_title：Journal of bacteriology

authors： Morohoshi T,Nakamura Y,Yamazaki G,Ishida A,Kato N,Ikeda T

更新日期：2007-11-01 00:00:00

abstract：:Enzymes of tyrosine biosynthesis (prephenate dehydrogenase and arogenate dehydrogenase) were characterized in 90 species currently classified within the genera Pseudomonas, Xanthomonas, and Alcaligenes. Variation in cofactor specificity and regulatory properties of the dehydrogenase proteins allowed the separation of ...

journal_title：Journal of bacteriology

authors： Byng GS,Whitaker RJ,Gherna RL,Jensen RA

更新日期：1980-10-01 00:00:00

abstract：:Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...

journal_title：Journal of bacteriology

authors： Jannasch HW

更新日期：1969-07-01 00:00:00

abstract：:The DNA sequence of a 3.23-kilobase fragment of the Escherichia coli chromosome encoding biosynthetic arginine decarboxylase (ADC) was determined. This sequence contained the speA open reading frame (ORF) as well as partial speB and metK ORFs. The ADC ORF is 1,974 nucleotides long; the deduced polypeptide contains 658...

journal_title：Journal of bacteriology

authors： Moore RC,Boyle SM

更新日期：1990-08-01 00:00:00

abstract：:The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...

journal_title：Journal of bacteriology

authors： Tsugawa A,Ohsumi Y,Kato I

更新日期：1970-10-01 00:00:00

abstract：:The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...

journal_title：Journal of bacteriology

authors： Chitlaru T,Gat O,Gozlan Y,Ariel N,Shafferman A

更新日期：2006-05-01 00:00:00

abstract：:Metal-dependent superoxide dismutases (SODs) with a specific requirement for a manganese or iron ion for catalytic activity and copper- and zinc-dependent enzymes are essential for detoxification of superoxide anion radicals. Genome sequence analyses predict the existence of a nickel-dependent enzyme (NiSOD) as the un...

journal_title：Journal of bacteriology

authors： Eitinger T

更新日期：2004-11-01 00:00:00

abstract：:Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found...

journal_title：Journal of bacteriology

authors： Tompkins TA,Barreau G,Broadbent JR

更新日期：2012-11-01 00:00:00

abstract：:We have characterized an acyl carrier protein (ACP) presumed to be involved in the synthesis of fatty acids in Streptomyces coelicolor A3(2). This is the third ACP to have been identified in S. coelicolor; the two previously characterized ACPs are involved in the synthesis of two aromatic polyketides: the blue-pigment...

journal_title：Journal of bacteriology

authors： Revill WP,Bibb MJ,Hopwood DA

更新日期：1996-10-01 00:00:00

abstract：:Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenin...

journal_title：Journal of bacteriology

authors： Mudd JB,Lee IM,Liu HY,Calavan EC

更新日期：1979-02-01 00:00:00

abstract：:Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

journal_title：Journal of bacteriology

authors： Graumann PL,Losick R

更新日期：2001-07-01 00:00:00

abstract：:An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putati...

journal_title：Journal of bacteriology

authors： Stephens RL,Haygood MG,Lidstrom ME

更新日期：1988-05-01 00:00:00

abstract：:We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...

journal_title：Journal of bacteriology

authors： Pacelli LZ,Edmiston SH,Mount DW

更新日期：1979-01-01 00:00:00

abstract：:We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

journal_title：Journal of bacteriology

authors： Tian H,Beckwith J

更新日期：2002-01-01 00:00:00

abstract：:Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

journal_title：Journal of bacteriology

authors： Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

更新日期：2005-11-01 00:00:00

abstract：:The inhibitory effect of fungitoxic derivatives of 1,4-oxathiin on substrate oxidation by the basidiomycete Ustilago maydis is diminished by a single-gene mutation (oxr). The difference between mutant and wild type is approximately the same on the basis of inhibition of either growth and operation of the tricarboxylic...

journal_title：Journal of bacteriology

authors： Georgopoulos SG,Alexandri E,Chrysayi M

更新日期：1972-06-01 00:00:00