Expression of the cloned uvrB gene of Escherichia coli: dependency on nonsense suppressors.

abstract：:Parallel studies were performed with methionineless derivatives of Escherichia coli 15 T(-) and Bacillus megaterium KM: T(-). Methylated bases are present in the total cell ribonucleic acid (RNA) of B. megaterium. The level of RNA methylation in E. coli is about 60% greater than that in B. megaterium. Although E. coli...

journal_title：Journal of bacteriology

authors： Wachsman JT,Irwin V

更新日期：1970-11-01 00:00:00

abstract：:Azotobacter vinelandii produces two detectable catalases during growth on minimal medium. The heat-labile catalase expressed during exponential growth phase was identified as a KatG homologue by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a mixed protein sample. The second catalase was heat resista...

journal_title：Journal of bacteriology

authors： Sandercock JR,Page WJ

更新日期：2008-02-01 00:00:00

abstract：:In Bacillus subtilis, the ermC gene encodes an mRNA that is unusually stable (40-min half-life) in the presence of erythromycin, an inducer of ermC gene expression. A requirement for this induced mRNA stability is a ribosome stalled in the ermC leader region. This property of ermC mRNA was used to study the decay of m...

journal_title：Journal of bacteriology

authors： Bechhofer DH,Zen KH

更新日期：1989-11-01 00:00:00

abstract：:The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In t...

journal_title：Journal of bacteriology

authors： Lee HS,Cho Y,Lee JH,Kang SG

更新日期：2008-04-01 00:00:00

abstract：:The actinomycin synthetases ACMS I, II, and III catalyze the assembly of the acyl peptide lactone precursor of actinomycin by a nonribosomal mechanism. We have cloned the genes of ACMS I (acmA) and ACMS II (acmB) by hybridization screening of a cosmid library of Streptomyces chrysomallus DNA with synthetic oligonucleo...

journal_title：Journal of bacteriology

authors： Schauwecker F,Pfennig F,Schröder W,Keller U

更新日期：1998-05-01 00:00:00

abstract：:The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique protein...

journal_title：Journal of bacteriology

authors： Chromy BA,Choi MW,Murphy GA,Gonzales AD,Corzett CH,Chang BC,Fitch JP,McCutchen-Maloney SL

更新日期：2005-12-01 00:00:00

abstract：:Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on ...

journal_title：Journal of bacteriology

authors： Tochikubo K

更新日期：1974-03-01 00:00:00

abstract：:Manohar, M. (University of Minnesota, St. Paul), S. Kumar, and R. K. Lindorfer. Heat reactivation of the alpha-hemolytic, dermonecrotic, and lethal activities of crude and purified staphylococcal alpha-toxin. J. Bacteriol. 91:1681-1685. 1966.-Crude staphylococcal toxin loses its alpha-hemolytic activity more rapidly a...

journal_title：Journal of bacteriology

authors： Manohar M,Kumar S,Lindorfer RK

更新日期：1966-05-01 00:00:00

abstract：:Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...

journal_title：Journal of bacteriology

authors： Perez E,Zheng H,Stock AM

更新日期：2006-06-01 00:00:00

abstract：:Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...

journal_title：Journal of bacteriology

authors： Hemmi H,Takahashi Y,Shibuya K,Nakayama T,Nishino T

更新日期：2005-03-01 00:00:00

abstract：UNLABELLED:The role of chromosomal toxin-antitoxin (TA) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. We have scanned the Vibrio cholerae N16961 genome for class 2 TA genes and identified 18 gene pair candidates. Interestingly, all but one are located in the chromosome 2 su...

journal_title：Journal of bacteriology

authors： Iqbal N,Guérout AM,Krin E,Le Roux F,Mazel D

更新日期：2015-07-01 00:00:00

abstract：:The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and ...

journal_title：Journal of bacteriology

authors： Legrand P,Bouche JP,Louarn JM

更新日期：1979-12-01 00:00:00

abstract：:Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...

journal_title：Journal of bacteriology

authors： Copeland JC

更新日期：1971-02-01 00:00:00

abstract：:The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

journal_title：Journal of bacteriology

authors： Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

更新日期：2009-02-01 00:00:00

abstract：:Saccharopolyspora spinosa is a Gram-positive bacterium that produces spinosad, a well-known biodegradable insecticide that is used for agricultural pest control and has an excellent environmental and mammalian toxicological profile. Here, we present the first draft genome sequence of the type strain Saccharopolyspora ...

journal_title：Journal of bacteriology

authors： Pan Y,Yang X,Li J,Zhang R,Hu Y,Zhou Y,Wang J,Zhu B

更新日期：2011-06-01 00:00:00

abstract：:Physiological studies of a mutant of Escherichia coli lacking the three lytic transglycosylases Slt70, MltA, and MltB revealed that interference with murein turnover can prevent AmpC beta-lactamase induction. The triple mutant, although growing normally, shows a dramatically reduced rate of murein turnover. Despite th...

journal_title：Journal of bacteriology

authors： Kraft AR,Prabhu J,Ursinus A,Höltje JV

更新日期：1999-12-01 00:00:00

abstract：:Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membr...

journal_title：Journal of bacteriology

authors： Mollerach ME,Partoune P,Coyette J,Ghuysen JM

更新日期：1996-03-01 00:00:00

abstract：:Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...

journal_title：Journal of bacteriology

authors： Bladen HA,Hampp EG

更新日期：1964-05-01 00:00:00

abstract：:The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...

journal_title：Journal of bacteriology

authors： Cluzel ME,Zanella-Cléon I,Cozzone AJ,Fütterer K,Duclos B,Molle V

更新日期：2010-12-01 00:00:00

abstract：:The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates o...

journal_title：Journal of bacteriology

authors： Lambert MP,Neuhaus FC

更新日期：1972-06-01 00:00:00

abstract：:In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...

journal_title：Journal of bacteriology

authors： Cook DN,Armstrong GA,Hearst JE

更新日期：1989-09-01 00:00:00

abstract：:The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derive...

journal_title：Journal of bacteriology

authors： Ke D,Boissinot M,Huletsky A,Picard FJ,Frenette J,Ouellette M,Roy PH,Bergeron MG

更新日期：2000-12-01 00:00:00

abstract：:It has been previously established that Thiobacillus neapolitanus fixes CO2 by using a form I ribulose bisphosphate carboxylase/oxygenase (RuBisCO), that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL and cbbS, are part of a putative carboxysome operon. In the present stud...

journal_title：Journal of bacteriology

authors： Baker SH,Jin S,Aldrich HC,Howard GT,Shively JM

更新日期：1998-08-01 00:00:00

abstract：:We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

journal_title：Journal of bacteriology

authors： Tian H,Beckwith J

更新日期：2002-01-01 00:00:00

abstract：:Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...

journal_title：Journal of bacteriology

authors： Miller RV,Kokjohn TA

更新日期：1988-05-01 00:00:00

abstract：:The regulatory proteins OmpR and EnvZ are both required to activate expression of the genes for the major outer membrane porin proteins, OmpF and OmpC, of Escherichia coli K-12. Here we show that OmpR, under certain conditions, could activate porin expression in the complete absence of EnvZ. In addition, the pleiotrop...

journal_title：Journal of bacteriology

authors： Slauch JM,Garrett S,Jackson DE,Silhavy TJ

更新日期：1988-01-01 00:00:00

abstract：:Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...

journal_title：Journal of bacteriology

authors： Beeser AE,Cooper TG

更新日期：1999-09-01 00:00:00

abstract：:The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

journal_title：Journal of bacteriology

authors： Stitt BL,Mosig G

更新日期：1989-07-01 00:00:00

abstract：:5-Azacytidine was found to be bactericidal to Escherichia coli carrying plasmids specifying EcoRII restriction-modification systems, but not to the same strains lacking these plasmids. Of other base analogs tested, only 5(beta-D-ribofuranosyl)isocytidine had similar, although weaker, effects. Plasmids that had lost th...

journal_title：Journal of bacteriology

authors： Friedman S

更新日期：1982-07-01 00:00:00

abstract：:The Haemophilus cryptic genospecies is an important cause of maternal genital tract and neonatal systemic infections and initiates infection by colonizing the genital or respiratory epithelium. In recent work, we identified a unique Haemophilus cryptic genospecies protein called Cha, which mediates efficient adherence...

journal_title：Journal of bacteriology

authors： Sheets AJ,St Geme JW 3rd

更新日期：2011-01-01 00:00:00