Methylated bases of Bacillus megaterium KM nucleic acids: comparison with Escherichia coli.

Abstract:

:Parallel studies were performed with methionineless derivatives of Escherichia coli 15 T(-) and Bacillus megaterium KM: T(-). Methylated bases are present in the total cell ribonucleic acid (RNA) of B. megaterium. The level of RNA methylation in E. coli is about 60% greater than that in B. megaterium. Although E. coli deoxyribonucleic acid (DNA) was found to contain 0.12% 5-methylcytosine (5-MC) and 0.24% 6-methylaminopurine (6-MA), methylated bases were not detected in the DNA of B. megaterium. Assuming a molecular weight of 7 x 10(9) daltons for B. megaterium DNA, it was calculated that this organism could not contain more than one molecule of 5-MC or 6-MA per genome, and that possibly no methylated bases were present. Methylated bases were also not detected in the DNA of thymine-starved B. megaterium. Crude extracts of this organism possess RNA methylase activity but no detectable DNA methylase activity.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wachsman JT,Irwin V

doi

10.1128/JB.104.2.814-818.1970

subject

Has Abstract

pub_date

1970-11-01 00:00:00

pages

814-8

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

104

pub_type

杂志文章
  • Genome sequence of the naturally plasmid-free Lactobacillus plantarum strain NC8 (CCUG 61730).

    abstract::Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many labor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00141-12

    authors: Axelsson L,Rud I,Naterstad K,Blom H,Renckens B,Boekhorst J,Kleerebezem M,van Hijum S,Siezen RJ

    更新日期:2012-05-01 00:00:00

  • Extent of host deletions associated with bacteriophage P2-mediated eduction.

    abstract::A series of independent Escherichia coli K eductants has been isolated and tested to determine the extent of their deletions. The deletions cover the P2 prophage in location H, the his operon, a suppressor of the recBC phenotype (sbcB), the gene for gluconate-6-phosphate dehydrogenase (gnd), a locus involved in cell w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.2.695-704.1971

    authors: Sunshine MG,Kelly B

    更新日期:1971-11-01 00:00:00

  • hisT is part of a multigene operon in Escherichia coli K-12.

    abstract::The Escherichia coli K-12 hisT gene has been cloned, and its organization and expression have been analyzed on multicopy plasmids. The hisT gene, which encodes tRNA pseudouridine synthase I (PSUI), was isolated on a Clarke-Carbon plasmid known to contain the purF gene. The presence of the hisT gene on this plasmid was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.60-71.1985

    authors: Marvel CC,Arps PJ,Rubin BC,Kammen HO,Penhoet EE,Winkler ME

    更新日期:1985-01-01 00:00:00

  • Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element.

    abstract::We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.8.2287-2298.1996

    authors: Zückert WR,Meyer J

    更新日期:1996-04-01 00:00:00

  • Global genomic analysis of Pseudomonas savastanoi pv. savastanoi plasmids.

    abstract::Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01067-07

    authors: Pérez-Martínez I,Zhao Y,Murillo J,Sundin GW,Ramos C

    更新日期:2008-01-01 00:00:00

  • Conditions affecting transformation of a group H streptococcus.

    abstract::Schlissel, Harvey J. (The University of Kansas, Lawrence), and C. P. Sword. Conditions affecting transformation of a group H streptococcus. J. Bacteriol. 92:1357-1363. 1966.-A defined transforming medium (DTM) containing buffer and 5 to 10 mug per ml of deoxyribonucleic acid was developed to study the physical and che...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.5.1357-1363.1966

    authors: Schlissel HJ,Sword CP

    更新日期:1966-11-01 00:00:00

  • Staphylococcal penicillinase. I. Inhibition and stimulation of activity.

    abstract::Saz, Arthur K. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Dolores L. Lowery, and Leah J. Jackson. Staphylococcal penicillinase. I. Inhibition and stimulation of activity. J. Bacteriol. 82:298-304. 1961.-The penicillinase extracted from a penicillin-resistant strain of Staphylococcus aureus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.2.298-304.1961

    authors: SAZ AK,LOWERY DL,JACKSON LJ

    更新日期:1961-08-01 00:00:00

  • In vitro sulfotransferase activity of NodH, a nodulation protein of Rhizobium meliloti required for host-specific nodulation.

    abstract::Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.21.6237-6245.1995

    authors: Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

    更新日期:1995-11-01 00:00:00

  • Allosteric regulation of the glucose:H+ symporter of Lactobacillus brevis: cooperative binding of glucose and HPr(ser-P).

    abstract::Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.7.1900-1902.1995

    authors: Ye JJ,Saier MH Jr

    更新日期:1995-04-01 00:00:00

  • Deciphering morphological determinants of the helix-shaped Leptospira.

    abstract::Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. Defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05695-11

    authors: Slamti L,de Pedro MA,Guichet E,Picardeau M

    更新日期:2011-11-01 00:00:00

  • Transcriptional analysis of the principal cell division gene, ftsZ, of Mycobacterium tuberculosis.

    abstract::Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.7.2540-2550.2005

    authors: Roy S,Ajitkumar P

    更新日期:2005-04-01 00:00:00

  • Gene structures and regulation of the alkane hydroxylase complex in Acinetobacter sp. strain M-1.

    abstract::In the long-chain n-alkane degrader Acinetobacter sp. strain M-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1819-1823.2001

    authors: Tani A,Ishige T,Sakai Y,Kato N

    更新日期:2001-03-01 00:00:00

  • Suppressed nonsense mutations in the araC gene of Escherichia coli provide three novel variant proteins.

    abstract::A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.3.1329-1338.1983

    authors: Schecter JB

    更新日期:1983-06-01 00:00:00

  • The bile response repressor BreR regulates expression of the Vibrio cholerae breAB efflux system operon.

    abstract::Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00584-08

    authors: Cerda-Maira FA,Ringelberg CS,Taylor RK

    更新日期:2008-11-01 00:00:00

  • Chemotaxis mutants of Spirochaeta aurantia.

    abstract::Five Spirochaeta aurantia chemotaxis mutants were isolated. One mutant (the che-101 mutant) never reversed, one (the che-200 mutant) flexed predominantly, two (the che-300 and che-400-1 mutants) exhibited elevated reversal frequencies, and one (the che-400 mutant) exhibited chemotactically unstimulated behavior simila...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.606-611.1989

    authors: Fosnaugh K,Greenberg EP

    更新日期:1989-01-01 00:00:00

  • Lysis of Vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme.

    abstract::Wolin, M. J. (University of Illinois, Urbana). Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bacteriol. 91:1781-1786. 1966.-Cell suspensions of Vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (EDTA) or lysozyme. Lysis occurs at alkaline pH and is prevented by 0.15 m N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1781-1786.1966

    authors: Wolin MJ

    更新日期:1966-05-01 00:00:00

  • Phosphatidylglycerolphosphate synthase expression in Schizosaccharomyces pombe is regulated by the phospholipid precursors inositol and choline.

    abstract::The enzyme phosphatidylglycerolphosphate synthase (PGPS; CDP-diacylglycerol glycerol 3-phosphate 3-phosphatidyltransferase; EC 2.7.8.5) catalyzes the committed step in the cardiolipin biosynthetic pathway. To study the regulation of PGPS in Schizosaccharomyces pombe, we characterized the enzyme biochemically. Maximum ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6132-6138.1991

    authors: Karkhoff-Schweizer RR,Kelly BL,Greenberg ML

    更新日期:1991-10-01 00:00:00

  • Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries.

    abstract::An understanding of the enzymatic reactions catalyzing the degradation of substituted phenols, a major group of environmental pollutants, is required for the development of biological methods for the decontamination of halophenol-polluted sites. We found that a flavomonooxygenase, pentachlorophenol hydroxylase, isolat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.9.2898-2902.1992

    authors: Xun L,Topp E,Orser CS

    更新日期:1992-05-01 00:00:00

  • EslB Is Required for Cell Wall Biosynthesis and Modification in Listeria monocytogenes.

    abstract::Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00553-20

    authors: Rismondo J,Schulz LM,Yacoub M,Wadhawan A,Hoppert M,Dionne MS,Gründling A

    更新日期:2021-01-25 00:00:00

  • Distribution and diversity of hsd genes in Escherichia coli and other enteric bacteria.

    abstract::We screened Salmonella typhimurium, Citrobacter freundii, Klebsiella pneumoniae, Shigella boydii, and many isolates of Escherichia coli for DNA sequences homologous to those encoding each of two unrelated type I restriction and modification systems (EcoK and EcoA). Both K- and A-related hsd genes were identified, but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1775-1782.1988

    authors: Daniel AS,Fuller-Pace FV,Legge DM,Murray NE

    更新日期:1988-04-01 00:00:00

  • Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824.

    abstract::A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.871-885.1994

    authors: Nair RV,Bennett GN,Papoutsakis ET

    更新日期:1994-02-01 00:00:00

  • Characterization of the membrane-bound succinic dehydrogenase of Micrococcus lysodeikticus.

    abstract::The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.1.230-238.1971

    authors: Pollock JJ,Linder R,Salton MR

    更新日期:1971-07-01 00:00:00

  • Genetic and molecular characteristics of Vir plasmids of bovine septicemic Escherichia coli.

    abstract::Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.758-769.1980

    authors: Lopez-Alvarez J,Gyles CL,Shipley PL,Falkow S

    更新日期:1980-02-01 00:00:00

  • Global mutational analysis of NtrC-like activators in Myxococcus xanthus: identifying activator mutants defective for motility and fruiting body development.

    abstract::The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.20.6083-6094.2003

    authors: Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

    更新日期:2003-10-01 00:00:00

  • Partial purification of a membrane-associated deoxyribonucleic acid complex from Mycoplasma gallisepticum.

    abstract::A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.1.495-501.1974

    authors: Maniloff J,Quinlan DC

    更新日期:1974-10-01 00:00:00

  • The YjbH protein of Bacillus subtilis enhances ClpXP-catalyzed proteolysis of Spx.

    abstract::The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01289-08

    authors: Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

    更新日期:2009-02-01 00:00:00

  • Interaction of Erp Protein of Mycobacterium tuberculosis with Rv2212 Enhances Intracellular Survival of Mycobacterium smegmatis.

    abstract:UNLABELLED:The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo Although attempts have been made to understand the function of Erp protein, its exact ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00120-16

    authors: Ganaie AA,Trivedi G,Kaur A,Jha SS,Anand S,Rana V,Singh A,Kumar S,Sharma C

    更新日期:2016-09-22 00:00:00

  • Cloning and insertional inactivation of the dye (sfrA) gene, mutation of which affects sex factor F expression and dye sensitivity of Escherichia coli K-12.

    abstract::Deletions of the Escherichia coli K-12 chromosome between trpR and thr render the bacterium sensitive to the dye toluidine blue (Dye-), and if male (Hfr or F'), the strain is sterile (Fex-), failing to donate F' or chromosomal markers and resistant to male-specific phages as a consequence of its inability to elaborate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.3.1309-1314.1983

    authors: Buxton RS,Drury LS

    更新日期:1983-06-01 00:00:00

  • Identification of the promoter region of the Escherichia coli major cold shock gene, cspA.

    abstract::The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10 degrees C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.12.3867-3873.1992

    authors: Tanabe H,Goldstein J,Yang M,Inouye M

    更新日期:1992-06-01 00:00:00

  • Multiple promoter inversions generate surface antigenic variation in Mycoplasma penetrans.

    abstract::Mycoplasma penetrans is a newly identified species of the genus MYCOPLASMA: It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON<==>OFF phase variations of the P35 family...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.231-242.2003

    authors: Horino A,Sasaki Y,Sasaki T,Kenri T

    更新日期:2003-01-01 00:00:00