Abstract:
:The enzyme phosphatidylglycerolphosphate synthase (PGPS; CDP-diacylglycerol glycerol 3-phosphate 3-phosphatidyltransferase; EC 2.7.8.5) catalyzes the committed step in the cardiolipin biosynthetic pathway. To study the regulation of PGPS in Schizosaccharomyces pombe, we characterized the enzyme biochemically. Maximum activity occurred in the presence of 6 mM Triton X-100 at pH 7.5. The apparent Km values for CDP-diacylglycerol and glycerol 3-phosphate were 130 and 26 microM, respectively. Optimal activity was at 35 degrees C, and enzyme activity was labile above 40 degrees C. Thioreactive agents were inhibitory to PGPS activity. To determine whether S. pombe PGPS is regulated by phospholipid precursors, we examined the time-dependent expression of PGPS upon inositol and choline starvation. Starvation for inositol resulted in a threefold increase in PGPS expression in wild-type cells. In cho1 and cho2 mutants, which are blocked in phosphatidylcholine synthesis, starvation for choline resulted in transient derepression of PGPS expression. In choline auxotrophs starved for inositol, PGPS was derepressed 2.5- to 3-fold in the presence of choline and less or not at all in the absence of choline. This is the first description of PGPS regulation in S. pombe and the first demonstration of inositol-mediated regulation in the inositol-requiring yeast species.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Karkhoff-Schweizer RR,Kelly BL,Greenberg MLdoi
10.1128/jb.173.19.6132-6138.1991keywords:
subject
Has Abstractpub_date
1991-10-01 00:00:00pages
6132-8issue
19eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::To isolate genes from Escherichia coli which regulate the labile hydrogenase activity, a plasmid library was used to transform hydL mutants lacking the labile hydrogenase. A single type of gene, designated hydG, was isolated. This gene also partially restored the hydrogenase activity in hydF mutants (which are defecti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.8.4448-4456.1989
更新日期:1989-08-01 00:00:00
abstract::The deletion of a gene coding for a histidine kinase (sll0750, Hik8) in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 resulted in a conditional lethal phenotype with a pleiotropic effect on the expression of genes involved in glucose metabolism. This mutant had comparable doubling times to wild type...
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更新日期:2005-04-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.94.1.125-130.1967
更新日期:1967-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4645-4651.1995
更新日期:1995-08-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1973-01-01 00:00:00
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更新日期:1994-07-01 00:00:00
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更新日期:1981-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.10.2729-2735.1998
更新日期:1998-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.179.15.4959-4961.1997
更新日期:1997-08-01 00:00:00
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doi:10.1128/jb.182.2.546-550.2000
更新日期:2000-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1375-1383.1993
更新日期:1993-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02045-14
更新日期:2014-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2835-2845.1994
更新日期:1994-05-01 00:00:00
abstract::While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00160-07
更新日期:2007-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.868-873.1984
更新日期:1984-03-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.84.4.793-796.1962
更新日期:1962-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1962-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.905-910.1975
更新日期:1975-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.7819-7827.1993
更新日期:1993-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
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更新日期:2012-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01701-07
更新日期:2008-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00728-06
更新日期:2006-12-01 00:00:00
abstract::The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.525-530.1990
更新日期:1990-02-01 00:00:00
abstract::The antibacterial protein toxin colicin V is secreted from Escherichia coli cells by a dedicated export system that is a member of the multicomponent ATP-binding cassette (ABC) transporter family. At least three proteins, CvaA, CvaB, and TolC, are required for secretion via this signal sequence-independent pathway. In...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6153-6159.1995
更新日期:1995-11-01 00:00:00
abstract::A DNA region carrying lysS, the gene encoding the lysyl-tRNA synthetase, was cloned from the extreme thermophile prokaryote Thermus thermophilus VK-1 and sequenced. The analysis indicated an open reading frame encoding a protein of 492 amino acids. This putative protein has significant homologies to previously sequenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.9.2699-2705.1994
更新日期:1994-05-01 00:00:00