Partial purification of a membrane-associated deoxyribonucleic acid complex from Mycoplasma gallisepticum.

Abstract:

:A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but not by saponin, ribonuclease, phospholipase C, or high-frequency sonic treatment. Sonic treatment further fractionates the cell ghost and allows partial purification, on sucrose density gradients, of a deoxyribonucleic acid replication complex attached to the cells' polar membrane-bleb-infrableb structures.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Maniloff J,Quinlan DC

doi

10.1128/JB.120.1.495-501.1974

subject

Has Abstract

pub_date

1974-10-01 00:00:00

pages

495-501

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

120

pub_type

杂志文章
  • AsnC: an autogenously regulated activator of asparagine synthetase A transcription in Escherichia coli.

    abstract::The regulation of the asparagine synthetase A gene of Escherichia coli was studied in vitro with a coupled transcription-translation system. It was shown that the 17-kilodalton gene, which is transcribed divergently from the adjacent asnA gene, codes for an activator of asnA transcription. The synthesis of the 17-kilo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.310-315.1985

    authors: Kölling R,Lother H

    更新日期:1985-10-01 00:00:00

  • INTRACELLULAR BEHAVIOR OF HISTOPLASMA CAPSULATUM.

    abstract::Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.33-38.1964

    authors: HOWARD DH

    更新日期:1964-01-01 00:00:00

  • Genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834.

    abstract::The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.6.1946-1950.1997

    authors: Irazabal N,Marín I,Amils R

    更新日期:1997-03-01 00:00:00

  • Domains within RbpA Serve Specific Functional Roles That Regulate the Expression of Distinct Mycobacterial Gene Subsets.

    abstract::The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RPo) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein, i.e., an N-terminal tail (NTT) that interacts with RNAP β' and σ subunits, a core domain (C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-17

    authors: Prusa J,Jensen D,Santiago-Collazo G,Pope SS,Garner AL,Miller JJ,Ruiz Manzano A,Galburt EA,Stallings CL

    更新日期:2018-06-11 00:00:00

  • Induction of alkane hydroxylase proteins by unoxidized alkane in Pseudomonas putida.

    abstract::In vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. Purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.2.759-760.1975

    authors: Benson S,Shapiro J

    更新日期:1975-08-01 00:00:00

  • Stabilization of the pertussis toxin secretion apparatus by the C terminus of PtlD.

    abstract::Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 7...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01106-08

    authors: Verma A,Cheung AM,Burns DL

    更新日期:2008-11-01 00:00:00

  • Lytic effects of staphylococcal alpha-toxin and delta-hemolysin.

    abstract::Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.2.487-491.1968

    authors: Bernheimer AW,Avigad LS,Grushoff P

    更新日期:1968-08-01 00:00:00

  • Identification and function of the pdxY gene, which encodes a novel pyridoxal kinase involved in the salvage pathway of pyridoxal 5'-phosphate biosynthesis in Escherichia coli K-12.

    abstract::pdrK encodes a pyridoxine (PN)/pyridoxal (PL)/pyridoxamine (PM) kinase thought to function in the salvage pathway of pyridoxal 5'-phosphate (PLP) coenzyme biosynthesis. The observation that pdxK null mutants still contain PL kinase activity led to the hypothesis that Escherichia coli K-12 contains at least one other B...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1814-1821.1998

    authors: Yang Y,Tsui HC,Man TK,Winkler ME

    更新日期:1998-04-01 00:00:00

  • Translational autoregulation of ermC 23S rRNA methyltransferase expression in Bacillus subtilis.

    abstract::ermC specifies an rRNA methyltransferase that confers resistance to erythromycin. The expression of this determinant is induced by the addition of erythromycin. The induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. We now show that synthesis of the ermC gene pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1133-1141.1986

    authors: Denoya CD,Bechhofer DH,Dubnau D

    更新日期:1986-12-01 00:00:00

  • Isocitrate lyase from a thermophilic Bacillus: effect of salts on enzyme activity.

    abstract::The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1160-1169.1973

    authors: Griffiths MW,Sundaram TK

    更新日期:1973-12-01 00:00:00

  • Glucose uptake by the cellulolytic ruminal anaerobe Bacteroides succinogenes.

    abstract::Glucose uptake by Bacteroides succinogenes S85 was measured under conditions that maintained anaerobiosis and osmotic stability. Uptake was inhibited by compounds which interfere with electron transport systems, maintenance of proton or metal ion gradients, or ATP synthesis. The most potent inhibitors were proton and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.500-506.1987

    authors: Franklund CV,Glass TL

    更新日期:1987-02-01 00:00:00

  • Inhibition of peptidoglycan biosynthesis at a postcytoplasmic reaction in a stable L-phase variant of Streptococcus faecium.

    abstract::Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.900-910.1978

    authors: Gregory WW,Gooder H

    更新日期:1978-09-01 00:00:00

  • Cell-Biological Studies of Osmotic Shock Response in Streptomyces spp.

    abstract::Most bacteria are likely to face osmotic challenges, but there is yet much to learn about how such environmental changes affect the architecture of bacterial cells. Here, we report a cell-biological study in model organisms of the genus Streptomyces, which are actinobacteria that grow in a highly polarized fashion to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00465-16

    authors: Fuchino K,Flärdh K,Dyson P,Ausmees N

    更新日期:2016-12-13 00:00:00

  • Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release.

    abstract::Neisseria gonorrhoeae (gonococci) and Neisseria meningitidis (meningococci) are human pathogens that cause gonorrhea and meningococcal meningitis, respectively. Both N. gonorrhoeae and N. meningitidis release a number of small peptidoglycan (PG) fragments, including proinflammatory PG monomers, although N. meningitidi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00437-16

    authors: Chan JM,Dillard JP

    更新日期:2016-10-07 00:00:00

  • Importance of carbon dioxide in the isolation of pneumococci.

    abstract::Austrian, Robert (The University of Pennsylvania School of Medicine, Philadelphia), and Patricia Collins. Importance of carbon dioxide in the isolation of pneumococci. J. Bacteriol. 92:1281-1284. 1966.-Of the strains of pneumococci isolated from man, 8% manifest a requirement for CO(2) if detectable growth is to occur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.5.1281-1284.1966

    authors: Austrian R,Collins P

    更新日期:1966-11-01 00:00:00

  • Kinetic characterization of the WalRKSpn (VicRK) two-component system of Streptococcus pneumoniae: dependence of WalKSpn (VicK) phosphatase activity on its PAS domain.

    abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01690-09

    authors: Gutu AD,Wayne KJ,Sham LT,Winkler ME

    更新日期:2010-05-01 00:00:00

  • Sequence analysis of the mosquitocidal toxin genes encoding 51.4- and 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297.

    abstract::The nucleotide sequences of a 3,479-base-pair HindIII DNA fragment from Bacillus sphaericus 2362 and a 2,940-base-pair fragment from strain 2297 were determined; only minor differences were detected between them. Each contained two open reading frames coding for proteins of 51.4 and 41.9 kilodaltons. Both proteins wer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.5.2045-2050.1988

    authors: Baumann L,Broadwell AH,Baumann P

    更新日期:1988-05-01 00:00:00

  • Localization and regulation of bacteriophage Mu promoters.

    abstract::Mu promoters active during the lytic cycle were located by isolating RNA at various times after induction of Mu prophages, radiolabeling it by capping in vitro, and hybridizing it to Mu DNA fragments on Southern blots. Signals were detected from four new promoters in addition to the previously characterized Pe (early)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.6.3440-3448.1989

    authors: Stoddard SF,Howe MM

    更新日期:1989-06-01 00:00:00

  • Bordetella pertussis major outer membrane porin protein forms small, anion-selective channels in lipid bilayer membranes.

    abstract::The major outer membrane protein of molecular weight 40,000 (the 40K protein) of a virulent isolate of Bordetella pertussis was purified to apparent homogeneity. The purified protein formed an oligomer band (of apparent molecular weight 90,000) on sodium dodecyl sulfate-polyacrylamide gels after solubilization at low ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.212-216.1986

    authors: Armstrong SK,Parr TR Jr,Parker CD,Hancock RE

    更新日期:1986-04-01 00:00:00

  • A bifunctional enzyme in Pseudomonas aeruginosa: a new pattern in the organization of enzymes concerned with phenylalanine and tyrosine biosynthesis.

    abstract::Two isozymes of chorismate mutase (CA mutase(1) and CA mutase(2)) and two isozymes of prephenate dehydratase (PPA dehydratase(1) and PPA dehydratase(2)) have been found in Pseudomonas aeruginosa. The activities CA mutase(2)-PPA dehydratase(2) catalyzing phenylalanine biosynthesis have been purified almost 40-fold and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.205-212.1973

    authors: Ahmed SI,Campbell JJ

    更新日期:1973-07-01 00:00:00

  • Identification of a ligand on the Wip1 bacteriophage highly specific for a receptor on Bacillus anthracis.

    abstract::Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00655-13

    authors: Kan S,Fornelos N,Schuch R,Fischetti VA

    更新日期:2013-10-01 00:00:00

  • Conjugative plasmids in Neisseria gonorrhoeae.

    abstract::A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.1.278-286.1978

    authors: Sox TE,Mohammed W,Blackman E,Biswas G,Sparling PF

    更新日期:1978-04-01 00:00:00

  • Characterization of the Pseudomonas aeruginosa transcriptional response to phenylalanine and tyrosine.

    abstract::Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen often associated with chronic infections in the lungs of individuals with the heritable disease cystic fibrosis (CF). Previous work from our laboratory demonstrated that aromatic amino acids within CF lung secretions (sputum) not only serve as carbon and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00112-10

    authors: Palmer GC,Palmer KL,Jorth PA,Whiteley M

    更新日期:2010-06-01 00:00:00

  • FAILURE OF ARSENATE TO UNCOUPLE THE PHOSPHOTRANSACETYLASE SYSTEM IN CLOSTRIDIUM ACIDIURICI.

    abstract::Sagers, Richard D. (Brigham Young University, Provo, Utah), Moshe Benziman, and Sigrid M. Klein. Failure of arsenate to uncouple the phosphotransacetylase system in Clostridium acidiurici. J. Bacteriol. 86:978-984. 1963.-The conversion of pyruvate to acetyl phosphate by extracts of Clostridium acidiurici required coen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.5.978-984.1963

    authors: SAGERS RD,BENZIMAN M,KLEIN SM

    更新日期:1963-11-01 00:00:00

  • Sequence-specific DNA uptake in transformation of Neisseria gonorrhoeae.

    abstract::Piliated, competent gonococci are known to preferentially take up homologous transforming DNA into the cell. We examined the mechanism for DNA uptake with pFA10, a hybrid 11.5-kilobase (kb) penicillin-resistant (Pcr) plasmid composed of heterologous DNA from a 7.2-kb Pcr plasmid and homologous DNA from a 4.2-kb gonoco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Graves JF,Biswas GD,Sparling PF

    更新日期:1982-12-01 00:00:00

  • Two segments in bacterial antizyme P22 are essential for binding and enhance degradation of lysine/ornithine decarboxylase in Selenomonas ruminantium.

    abstract::In Selenomonas ruminantium, a strictly anaerobic and gram-negative bacterium, the degradation of lysine/ornithine decarboxylase (LDC/ODC) by ATP-requiring protease(s) is accelerated by the binding of P22, which is a ribosomal protein of this strain. Amino acid sequence alignment of S. ruminantium P22 with the L10 ribo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01429-07

    authors: Yamaguchi Y,Takatsuka Y,Kamio Y

    更新日期:2008-01-01 00:00:00

  • Genome sequence of Mycoplasma iowae strain 695, an unusual pathogen causing deaths in turkeys.

    abstract::Mycoplasma iowae is associated mainly with reduced hatchability in turkeys and is well known for the unusual ability of phenotypic variation in the Mycoplasma surface components as well as a relative resistance to heat, bile salts, and many antimicrobials. A subset of unique genes and a gene cluster responsible for th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06297-11

    authors: Wei S,Guo Z,Li T,Zhang T,Li X,Zhou Z,Li Z,Liu M,Luo R,Bi D,Chen H,Zhou R,Jin H

    更新日期:2012-01-01 00:00:00

  • Genomic insights into the emerging human pathogen Mycobacterium massiliense.

    abstract::Mycobacterium massiliense (Mycobacterium abscessus group) is an emerging pathogen causing pulmonary disease and skin and soft tissue infections. We report the genome sequence of the type strain CCUG 48898. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01200-12

    authors: Tettelin H,Sampaio EP,Daugherty SC,Hine E,Riley DR,Sadzewicz L,Sengamalay N,Shefchek K,Su Q,Tallon LJ,Conville P,Olivier KN,Holland SM,Fraser CM,Zelazny AM

    更新日期:2012-10-01 00:00:00

  • Identification of long intergenic repeat sequences associated with DNA methylation sites in Caulobacter crescentus and other alpha-proteobacteria.

    abstract::A systematic search for motifs associated with CcrM DNA methylation sites revealed four long (>100-bp) motifs (CIR sequences) present in up to 21 copies in Caulobacter crescentus. The CIR1 and CIR2 motifs exhibit a conserved inverted repeat organization, with a CcrM site in the center of one of the repeats. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.16.4997-5002.2003

    authors: Chen SL,Shapiro L

    更新日期:2003-08-01 00:00:00

  • Unusual sulfonolipids are characteristic of the Cytophaga-Flexibacter group.

    abstract::Capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (W. Godchaux III and E. R. Leadbetter, J. Bacteriol. 144:592-602, 1980). One of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, N-acylated versions of capnine. The lipids were foun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.3.1238-1246.1983

    authors: Godchaux W 3rd,Leadbetter ER

    更新日期:1983-03-01 00:00:00