Abstract:
:ermC specifies an rRNA methyltransferase that confers resistance to erythromycin. The expression of this determinant is induced by the addition of erythromycin. The induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. We now show that synthesis of the ermC gene product in Bacillus subtilis is also autoregulated by a mechanism operating on the level of translation. The synthesis of methyltransferase was shown to be gene dosage compensated by Western blot analysis. Several mutants were analyzed that specify altered ermC gene products and are deregulated. Analysis of mutants and of the wild-type strain by Northern blotting demonstrated that autoregulation is posttranscriptional. We suggest a translational repression model in which the ermC methyltransferase binds to its own mRNA, at a region that resembles the methylation target site on 23S rRNA. The overall control of ermC expression is discussed in light of these multiple regulatory mechanisms.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Denoya CD,Bechhofer DH,Dubnau Ddoi
10.1128/jb.168.3.1133-1141.1986subject
Has Abstractpub_date
1986-12-01 00:00:00pages
1133-41issue
3eissn
0021-9193issn
1098-5530journal_volume
168pub_type
杂志文章abstract::Functional domain structures of pyocins AP41, S1, and S2 were assigned by examining the functions of chimeric pyocins and deletion derivatives. Pyocins AP41, S1, and S2 are essentially composed of three domains, the receptor-binding domain, the translocation domain, and the DNase domain, in that order from the N termi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6179-6185.1993
更新日期:1993-10-01 00:00:00
abstract::The urease activity of Ureaplasma urealyticum was found to be located in the cytoplasmic fraction and consisted of multiple stable enzyme forms representing at least four biotypes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1390-1392.1977
更新日期:1977-06-01 00:00:00
abstract::A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.468-470.1988
更新日期:1988-01-01 00:00:00
abstract::Cyanothece sp. strain ATCC 51142 is a unicellular, diazotrophic cyanobacterium which demonstrated extensive metabolic periodicities of photosynthesis, respiration, and nitrogen fixation when grown under N2-fixing conditions. N2 fixation and respiration peaked at 24-h intervals early in the dark or subjective-dark peri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.13.4319-4327.1997
更新日期:1997-07-01 00:00:00
abstract::Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.333-341.1979
更新日期:1979-11-01 00:00:00
abstract::From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.1090-1094.1980
更新日期:1980-08-01 00:00:00
abstract::We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4047-4054.1988
更新日期:1988-09-01 00:00:00
abstract::Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.284-296.1962
更新日期:1962-02-01 00:00:00
abstract::Autoinduction is a conserved mechanism of cell density-dependent gene regulation that occurs in a variety of gram-negative bacteria. Autoinducible luminescence in Vibrio fischeri requires a transcriptional activator, LuxR, while a LuxR homolog, LasR, activates elastase expression in Pseudomonas aeruginosa. Both LuxR a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.3076-3080.1994
更新日期:1994-05-01 00:00:00
abstract::The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.2.764-774.1975
更新日期:1975-11-01 00:00:00
abstract::Our whole-genome microarray studies of Neisseria meningitidis MC58 previously identified a set of 153 genes whose transcription was activated during growth in iron. In this study, Fur-mediated regulation of the iron-activated nspA gene was confirmed, whereas iron-activated regulation of the secY gene was demonstrated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01638-06
更新日期:2007-01-01 00:00:00
abstract::Mycoplasma fermentans is a microorganism commonly found in the genitourinary and respiratory tracts of healthy individuals and AIDS patients. The complete genome of the repetitive-sequence-rich M. fermentans strain M64 is reported here. Comparative genomics analysis revealed dramatic differences in genome size between...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05228-11
更新日期:2011-08-01 00:00:00
abstract::We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.16.4771-4778.2001
更新日期:2001-08-01 00:00:00
abstract::Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be posttranslationally modified for full functionality; in enterobacteria, this is accomplished by two enzymes, namely, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00722-18
更新日期:2019-05-08 00:00:00
abstract::The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.411-415.1980
更新日期:1980-07-01 00:00:00
abstract::Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.530-540.1992
更新日期:1992-01-01 00:00:00
abstract::Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from many bacterial sources were analyzed. It was shown that whereas gram-negative bacteria have only one murA gene, gram-positive bacteria have two distinct genes encoding these enzymes which have possibly arisen from gene dupl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.15.4146-4152.2000
更新日期:2000-08-01 00:00:00
abstract::A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.4.1319-1323.1999
更新日期:1999-02-01 00:00:00
abstract::The enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum grown on crystalline cellulose as a sole carbon and energy source was explored by two-dimensional electrophoresis. The cellulolytic system of C. cellulolyticum is composed of at least 30 dockerin-containing proteins (designated cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00917-06
更新日期:2007-03-01 00:00:00
abstract::To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.1.280-283.1997
更新日期:1997-01-01 00:00:00
abstract::The major surface-located protein in the outer membrane of Haemophilus influenzae type b (Hib) is porin, molecular mass, 38 kDa, 341 amino acids. To define precisely the molecular reactivities of nine mouse monoclonal antibodies (MAbs) against Hib porin, overlapping hexapeptides corresponding to the entire sequence of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.12.4007-4016.1992
更新日期:1992-06-01 00:00:00
abstract::In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.11.3345-3353.1994
更新日期:1994-06-01 00:00:00
abstract::Adler, H. E. (University of California, Davis), M. Shifrine, and Herrad Ortmayer. Mycoplasma inocuum sp. n., a saprophyte from chickens. J. Bacteriol. 82:239-240. 1961.-A new species of Mycoplasma was isolated from the infraorbital sinuses of chickens. The organism, a saprophyte, differs from all known mycoplasmas of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.2.239-240.1961
更新日期:1961-08-01 00:00:00
abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01238-07
更新日期:2007-12-01 00:00:00
abstract::TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.23.7274-7279.1997
更新日期:1997-12-01 00:00:00
abstract::ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5414-5418.1991
更新日期:1991-09-01 00:00:00
abstract::Berry, L. Joe (Bryn Mawr College, Bryn Mawr, Pa.), Dorothy S. Smythe, and Louise S. Colwell. Inhibition of inducible liver enzymes by endotoxin and actinomycin D. J. Bacteriol. 92:107-115. 1966.-Bacterial endotoxin at the ld(50) level lowers liver tryptophan pyrrolase in mice, it prevents for 16 to 20 hr the induction...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.1.107-115.1966
更新日期:1966-07-01 00:00:00
abstract::Glycerol:NAD+2-OXIDOREDUCTASE (EC 1.1.1.6) was purified to homogeneity from a mutant of Escherichia coli K12 that uses this enzyme, instead of ATP:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.1.182-187.1979
更新日期:1979-10-01 00:00:00
abstract::The model archaeon Pyrococcus furiosus grows optimally near 100°C on carbohydrates and peptides. Its genome sequence (NCBI) was determined 12 years ago. A genetically tractable strain, COM1, was very recently reported, and here we describe its genome sequence. Of 1,909,827 bp in size, it is 1,571 bp longer (0.1%) than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00439-12
更新日期:2012-08-01 00:00:00
abstract::Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.237-244.1967
更新日期:1967-01-01 00:00:00