Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH.

Abstract:

:Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as cadA. One of these, cadB, is 444 codons long and is situated immediately upstream of cadA. Transcriptional fusions between fragments upstream of cadA and lacZ, Northern (RNA) hybridization, primer extension, and site-directed mutagenesis experiments defined a promoter, Pcad, upstream of cadB that was responsible for pH-regulated expression of cadA. Upstream of Pcad is an open reading frame, cadC, consisting of 512 codons. The predicted amino terminal region of the cadC gene product (CadC) resembles the carboxy-terminal domain of prokaryotic transcriptional activators involved in environmental sensing. Tn10 insertions within or immediately upstream of cadC abolished Pcad activity, suggesting that cadC encodes a positive transcription factor. Expression of plasmid-borne cadC in the Tn10 mutants restored Pcad activity, while introduction of a plasmid expressing truncated CadC resulted in the inability to complement. The presence of Pcad on a multicopy plasmid was found to lower expression arising from chromosomal Pcad, suggesting that a positive-acting factor is limiting. Our data suggests that cadA, cadB, and the acid-inducible Pcad comprise, at least in part, the cad operon which is under control of the cadC product.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Watson N,Dunyak DS,Rosey EL,Slonczewski JL,Olson ER

doi

10.1128/jb.174.2.530-540.1992

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

530-40

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

杂志文章
  • Inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function in wild-type Escherichia coli and Salmonella enterica.

    abstract::The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00970-10

    authors: O'Connor M,Gregory ST

    更新日期:2011-01-01 00:00:00

  • Modulation of luminescence operon expression by N-octanoyl-L-homoserine lactone in ainS mutants of Vibrio fischeri.

    abstract::Population density-dependent expression of luminescence in Vibrio fischeri is controlled by the autoinducer N-3-oxohexanoyl-L-homoserine lactone (autoinducer 1 [AI-1]), which via LuxR activates transcription of the lux operon (luxICDABEG, encoding the putative autoinducer synthase [LuxI] and the luminescence enzymes)....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.4.971-976.1996

    authors: Kuo A,Callahan SM,Dunlap PV

    更新日期:1996-02-01 00:00:00

  • Draft genome sequence of Sporolactobacillus inulinus strain CASD, an efficient D-lactic acid-producing bacterium with high-concentration lactate tolerance capability.

    abstract::Sporolactobacillus inulinus CASD is an efficient D-lactic acid producer with high optical purity. Here we report for the first time the draft genome sequence of S. inulinus (2,930,096 bp). The large number of annotated two-component system genes makes it possible to explore the mechanism of extraordinary lactate toler...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05934-11

    authors: Yu B,Su F,Wang L,Xu K,Zhao B,Xu P

    更新日期:2011-10-01 00:00:00

  • Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirochete.

    abstract::Spirochete MA-2, which is anaerobic, ferments glucose, forming acetate as a major product. The spirochete also ferments (but does not utilize as growth substrates) small amounts of l-leucine, l-isoleucine, and l-valine, forming the branched-chain fatty acids isovalerate, 2-methylbutyrate, and isobutyrate, respectively...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Harwood CS,Canale-Parola E

    更新日期:1982-10-01 00:00:00

  • Dynamics of the SetCD-regulated integration and excision of genomic islands mobilized by integrating conjugative elements of the SXT/R391 family.

    abstract::Mobilizable genomic islands (MGIs) are small genomic islands that are mobilizable by SXT/R391 integrating conjugative elements (ICEs) due to similar origins of transfer. Their site-specific integration and excision are catalyzed by the integrase that they encode, but their conjugative transfer entirely depends upon th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01093-12

    authors: Daccord A,Mursell M,Poulin-Laprade D,Burrus V

    更新日期:2012-11-01 00:00:00

  • Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida.

    abstract::Formylglutamate amidohydrolase (FGase) catalyzes the terminal reaction in the five-step pathway for histidine utilization in Pseudomonas putida. By this action, N-formyl-L-glutamate (FG) is hydrolyzed to produce L-glutamate plus formate. Urocanate, the first product in the pathway, induced all five enzymes, but FG was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4696-4702.1987

    authors: Hu L,Mulfinger LM,Phillips AT

    更新日期:1987-10-01 00:00:00

  • Structural insights into the regulatory mechanism of the response regulator RocR from Pseudomonas aeruginosa in cyclic Di-GMP signaling.

    abstract::The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00560-12

    authors: Chen MW,Kotaka M,Vonrhein C,Bricogne G,Rao F,Chuah ML,Svergun D,Schneider G,Liang ZX,Lescar J

    更新日期:2012-09-01 00:00:00

  • Oxidation of aliphatic olefins by toluene dioxygenase: enzyme rates and product identification.

    abstract::Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.3858-3865.1997

    authors: Lange CC,Wackett LP

    更新日期:1997-06-01 00:00:00

  • Autogenous transcriptional regulation of the regA gene, encoding an AraC-Like, essential virulence regulator in Citrobacter rodentium.

    abstract::We identified several promoters responsible for the expression of regA, which encodes a global virulence regulator in Citrobacter rodentium. Expression of some of the promoters was strongly autoactivated by RegA in conjunction with bicarbonate. Biochemical and mutational analyses were used to determine the consensus s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01224-10

    authors: Tan A,Yang J,Tauschek M,Praszkier J,Robins-Browne RM

    更新日期:2011-04-01 00:00:00

  • Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans.

    abstract::A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.1.410-413.1982

    authors: Sherman LA,van de Putte P

    更新日期:1982-04-01 00:00:00

  • Molecular and expression analysis of the Rhizobium meliloti phosphoenolpyruvate carboxykinase (pckA) gene.

    abstract::The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.6.1452-1460.1995

    authors: Osterås M,Driscoll BT,Finan TM

    更新日期:1995-03-01 00:00:00

  • Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus.

    abstract::The plant cell wall, which consists of a highly complex array of interconnecting polysaccharides, is the most abundant source of organic carbon in the biosphere. Microorganisms that degrade the plant cell wall synthesize an extensive portfolio of hydrolytic enzymes that display highly complex molecular architectures. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01701-07

    authors: DeBoy RT,Mongodin EF,Fouts DE,Tailford LE,Khouri H,Emerson JB,Mohamoud Y,Watkins K,Henrissat B,Gilbert HJ,Nelson KE

    更新日期:2008-08-01 00:00:00

  • Abnormal septation and inhibition of sporulation by accumulation of L- -glycerophosphate in Bacillus subtilis mutants.

    abstract::Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.1034-1045.1973

    authors: O YK,Freese EB,Freese E

    更新日期:1973-02-01 00:00:00

  • Regulatory effect of hydrogen on nitrogenase activity of the blue-green alga (cyanobacterium) Nostoc muscorum.

    abstract::Preincubation of the blue-green alga (cyanobacterium) Nostoc muscorum under an atmosphere of argon plus acetylene in the light led to a greater than fourfold increase of light-induced hydrogen evolution and to a 50% increase of acetylene reduction, as compared to cells that had not been preconditioned. The basic and t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1037-1040.1980

    authors: Scherer S,Kerfin W,Böger P

    更新日期:1980-03-01 00:00:00

  • Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1.

    abstract::Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01654-07

    authors: Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G

    更新日期:2008-04-01 00:00:00

  • Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR.

    abstract:UNLABELLED:Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00820-15

    authors: Uhde A,Brühl N,Goldbeck O,Matano C,Gurow O,Rückert C,Marin K,Wendisch VF,Krämer R,Seibold GM

    更新日期:2016-07-28 00:00:00

  • Regulation of formation of aerial mycelia and spores of Streptomyces viridochromogenes.

    abstract::Growth of Streptomyces viridochromogenes on a solid glycerol-NH4NO3 salts medium was accompanied by the formation of aerial mycelia and spores. Adding 0.5% or more casein hydrolysate to the medium stimulated growth while completely repressing the formation of aerial mycelia and spores. This repression was temporary, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1102-1111.1982

    authors: Coleman RH,Ensign JC

    更新日期:1982-03-01 00:00:00

  • Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.

    abstract::We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4488-4500.1995

    authors: Strohmaier H,Remler P,Renner W,Högenauer G

    更新日期:1995-08-01 00:00:00

  • Overexpression of vsr in Escherichia coli is mutagenic.

    abstract::Overexpression of vsr in Escherichia coli stimulates transition and frameshift mutations. The pattern of mutations suggests that mutagenesis is due to saturation or inactivation of dam-directed mismatch repair. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.14.4294-4296.1996

    authors: Doiron KM,Viau S,Koutroumanis M,Cupples CG

    更新日期:1996-07-01 00:00:00

  • Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: extensive allelic diversity and recombinational population structure.

    abstract::Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.13.3934-3938.1996

    authors: Go MF,Kapur V,Graham DY,Musser JM

    更新日期:1996-07-01 00:00:00

  • Identification and Characterization of the Major Porin of Desulfovibrio vulgaris Hildenborough.

    abstract::Due in large part to their ability to facilitate the diffusion of a diverse range of solutes across the outer membrane (OM) of Gram-negative bacteria, the porins represent one of the most prominent and important bacterial membrane protein superfamilies. Notably, for the Gram-negative bacterium Desulfovibrio vulgaris H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00286-17

    authors: Zeng L,Wooton E,Stahl DA,Walian PJ

    更新日期:2017-10-31 00:00:00

  • Caulobacter flagellar organelle: synthesis, compartmentation, and assembly.

    abstract::In Caulobacter crescentus biogenesis of the flagellar organelle occurs during one stage of its complex life cycle. Thus in synchronous cultures it is possible to assay the sequential synthesis and assembly of the flagellum and hook in vivo with a combination of biochemical and radioimmunological techniques. The period...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.1062-1069.1978

    authors: Lagenaur C,Agabian N

    更新日期:1978-09-01 00:00:00

  • Role of transmembrane pH gradient and membrane binding in nisin pore formation.

    abstract::Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.1.135-140.1997

    authors: Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

    更新日期:1997-01-01 00:00:00

  • Mitochondrial translation products during release from glucose repression in Saccharomyces cerevisiae.

    abstract::Mitochondrial protein synthesis was studied during release from glucose repression in Saccharomyces cerevisiae cells bearing different mitochondrial genomes. The increase in the rate of the synthesis of mitochondrial translation products was analyzed during respiratory induction. Different kinetic patterns were found ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.3.1125-1132.1983

    authors: Falcone C,Agostinelli M,Frontali L

    更新日期:1983-03-01 00:00:00

  • Site-directed mutagenesis of the mecA gene from a methicillin-resistant strain of Staphylococcus aureus.

    abstract::The mecA-27r gene from Staphylococcus aureus 27r encodes penicillin-binding protein 2a (PBP2a-27r), which causes this strain to be methicillin resistant. Removal or replacement of the N-terminal transmembrane domain had no effect on binding of penicillin, but removal of portions of the putative transglycosylase domain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.2.443-449.1994

    authors: Wu CY,Alborn WE Jr,Flokowitsch JE,Hoskins J,Unal S,Blaszczak LC,Preston DA,Skatrud PL

    更新日期:1994-01-01 00:00:00

  • Spontaneous emergence of an Hfr strain with a cit plasmid from natural isolates of citrate-positive Escherichia coli bovine origin.

    abstract::From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.1090-1094.1980

    authors: Ishiguro N,Oka C,Asagi M,Sato G,Yoshikawa M

    更新日期:1980-08-01 00:00:00

  • A gene and its product required for transposition of resistance transposon Tn2603.

    abstract::Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.2.723-728.1982

    authors: Tanaka M,Harafuji H,Yamamoto T

    更新日期:1982-08-01 00:00:00

  • ROLE OF MARINE FUNGI IN THE BIOCHEMISTRY OF THE OCEANS. IV. GROWTH RESPONSES TO SEAWATER INORGANIC MACROCONSTITUENTS.

    abstract::Sguros, Peter L. (University of Miami, Miami, Fla.), and Jacqueline Simms. Role of marine fungi in the biochemistry of the oceans. IV. Growth responses to seawater inorganic macroconstituents. J. Bacteriol. 88:346-355. 1964.-The filamentous ascomycete Halosphaeria mediosetigera and the deuteromycete Culcitalna achrasp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.2.346-355.1964

    authors: SGUROS PL,SIMMS J

    更新日期:1964-08-01 00:00:00

  • New type of osmoregulated solute transporter identified in halophilic members of the bacteria domain: TRAP transporter TeaABC mediates uptake of ectoine and hydroxyectoine in Halomonas elongata DSM 2581(T).

    abstract::The halophilic bacterium Halomonas elongata synthesizes as its main compatible solute the aspartate derivative ectoine. We constructed a deletion mutant of H. elongata, KB1, defective in ectoine synthesis and tolerating elevated salt concentrations only in the presence of external compatible solutes. The dependency of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.11.3078-3085.2002

    authors: Grammann K,Volke A,Kunte HJ

    更新日期:2002-06-01 00:00:00

  • Comparison of lipopolysaccharides from Agmenellum quadruplicatum to Escherichia coli and Salmonella typhimurium by using thin-layer chromatography.

    abstract::Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1566-1573.1975

    authors: Buttke TM,Ingram LO

    更新日期:1975-12-01 00:00:00