Spontaneous emergence of an Hfr strain with a cit plasmid from natural isolates of citrate-positive Escherichia coli bovine origin.

abstract：:Leucine and isoleucine-valine loci have been mapped in Rhizobium japonicum. Transformation analysis suggests a common pathway for isoleucine-valine biosynthesis. Three-point reciprocal crosses indicated that all the leucine loci are not genetically linked. ...

journal_title：Journal of bacteriology

authors： Doctor F,Modi VV

更新日期：1976-05-01 00:00:00

abstract：:Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...

journal_title：Journal of bacteriology

authors： da Silva SM,Pimentel C,Valente FM,Rodrigues-Pousada C,Pereira IA

更新日期：2011-06-01 00:00:00

abstract：:The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...

journal_title：Journal of bacteriology

authors： Baxter BK,Craig EA

更新日期：1998-12-01 00:00:00

abstract：:This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...

journal_title：Journal of bacteriology

authors： Vakharia H,German GJ,Misra R

更新日期：2001-12-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...

journal_title：Journal of bacteriology

authors： Bellanger X,Roberts AP,Morel C,Choulet F,Pavlovic G,Mullany P,Decaris B,Guédon G

更新日期：2009-04-01 00:00:00

abstract：:The type III secretion system (T3SS) of Pseudomonas aeruginosa is an important virulence factor. The T3SS of P. aeruginosa can be induced by a low calcium signal or upon direct contact with the host cells. The exact pathway of signal sensing and T3SS activation is not clear. By screening a transposon insertion mutant ...

journal_title：Journal of bacteriology

authors： Wu W,Badrane H,Arora S,Baker HV,Jin S

更新日期：2004-11-01 00:00:00

abstract：:The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) an...

journal_title：Journal of bacteriology

authors： Narberhaus F,Weiglhofer W,Fischer HM,Hennecke H

更新日期：1996-09-01 00:00:00

abstract：:After the demonstration that Stigmatella aurantiaca DW4 secretes an endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans (S. Bourgerie, Y. Karamanos, T. Grard, and R. Julien, J. Bacteriol. 176:6170-6174, 1994), an ENGase activity having the same substrate specifi...

journal_title：Journal of bacteriology

authors： Barreaud JP,Bourgerie S,Julien R,Guespin-Michel JF,Karamanos Y

更新日期：1995-02-01 00:00:00

abstract：:The Salmonella effector protein SopA is translocated into host cells via the SPI-1 type III secretion system (TTSS) and contributes to enteric disease. We found that the chaperone InvB binds to SopA and slightly stabilizes it in the bacterial cytosol and that it is required for its transport via the SPI-1 TTSS. ...

journal_title：Journal of bacteriology

authors： Ehrbar K,Hapfelmeier S,Stecher B,Hardt WD

更新日期：2004-02-01 00:00:00

abstract：:We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...

journal_title：Journal of bacteriology

authors： Freifelder D,Folkmanis A,Kirschner I

更新日期：1971-03-01 00:00:00

abstract：:A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1, 4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA g...

journal_title：Journal of bacteriology

authors： Suvarna K,Stevenson D,Meganathan R,Hudspeth ME

更新日期：1998-05-01 00:00:00

abstract：:Genetic studies have shown that the 53-kDa (Exo53) and 49-kDa (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid homology, Exo53 has been shown to express ADP-ribosyltransferase activity at abou...

journal_title：Journal of bacteriology

authors： Liu S,Yahr TL,Frank DW,Barbieri JT

更新日期：1997-03-01 00:00:00

abstract：:The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...

journal_title：Journal of bacteriology

authors： Lillie SH,Pringle JR

更新日期：1980-09-01 00:00:00

abstract：:The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which is mediated by short-chain fatty acids. During this fatty acid-mediated expression of spv, RpoS is required for inducti...

journal_title：Journal of bacteriology

authors： El-Gedaily A,Paesold G,Chen CY,Guiney DG,Krause M

更新日期：1997-02-01 00:00:00

abstract：:The membrane effects of chlorpromazine, nupercain, tetracain, and procain were studied using Bacillus cereus, B. megaterium, B. subtilis, and Streptococcus faecalis, protoplasts from S. faecalis, and isolated membranes from B. subtilis. Chlorpromazin, nupercain, and tetracain produced characteristic micromorphological...

journal_title：Journal of bacteriology

authors： Silva MT,Sousa JC,Polónia JJ,Macedo PM

更新日期：1979-01-01 00:00:00

abstract：:Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

journal_title：Journal of bacteriology

authors： Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

更新日期：2006-08-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Immunogenic activity of a ribosomal fraction obtained from Mycobacterium tuberculosis. J. Bacteriol. 89:1291-1298. 1965.-The highly immunogenic particulate fraction obtained from mechanically ruptured cells of the H37Ra strai...

journal_title：Journal of bacteriology

authors： YOUMANS AS,YOUMANS GP

更新日期：1965-05-01 00:00:00

abstract：:A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could...

journal_title：Journal of bacteriology

authors： Ray PH,Benedict CD

更新日期：1980-04-01 00:00:00

abstract：:Two types of mutants unable to excrete hemolysin were obtained when E. coli 5K carrying the multicopy hemolytic recombinant plasmid pANN202-312 was mutagenized with Mu d1. One type is altered in the plasmid hly-specific gene, hlyBb, but the other is caused by an insertion of Mu d1 into a chromosomal locus. ...

journal_title：Journal of bacteriology

authors： Juarez A,Goebel W

更新日期：1984-09-01 00:00:00

abstract：:The endoplasmic reticulum from Neurospora crassa was identified by monitoring the activity of the putative enzyme marker phosphatidylcholine glyceride transferase. After differential centrifugation of a cell homogenate, phosphatidylcholine glyceride transferase activity initially copurified with plasma membrane H+-ATP...

journal_title：Journal of bacteriology

authors： Borgeson CE,Bowman BJ

更新日期：1983-10-01 00:00:00

abstract：:Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. T...

journal_title：Journal of bacteriology

authors： Tam JE,Pattee PA

更新日期：1986-11-01 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the impo...

journal_title：Journal of bacteriology

authors： Rosey EL,Kennedy MJ,Petrella DK,Ulrich RG,Yancey RJ Jr

更新日期：1995-10-01 00:00:00

abstract：:Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine. oleY codes for a methyltransferase involved in the biosynthesis of L-oleandrose. This gene was overexpressed in Escherichia coli to form inclusion bodies and in Strept...

journal_title：Journal of bacteriology

authors： Rodríguez L,Rodríguez D,Olano C,Braña AF,Méndez C,Salas JA

更新日期：2001-09-01 00:00:00

abstract：:Groman, Neal B. (University of Washington, Seattle) and Grace Suzuki. Quantitative study of endolysin synthesis during reproduction of lambda phages. J. Bacteriol. 86:187-194. 1963.-Endolysin is presumed to be a phage-induced enzyme participating in lysis through its destructive action on the host cell wall. A method ...

journal_title：Journal of bacteriology

authors： GROMAN NB,SUZUKI G

更新日期：1963-08-01 00:00:00

abstract：:Exfoliative toxin type B is produced by bacteriophage group II strains of Staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. In addition to exfoliative toxin B, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. These phenotypes, as well as re...

journal_title：Journal of bacteriology

authors： Jackson MP,Iandolo JJ

更新日期：1986-05-01 00:00:00

abstract：:FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...

journal_title：Journal of bacteriology

authors： Srinivas S,Cronan JE

更新日期：2017-04-25 00:00:00

abstract：:The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...

journal_title：Journal of bacteriology

authors： Köplin R,Arnold W,Hötte B,Simon R,Wang G,Pühler A

更新日期：1992-01-01 00:00:00

abstract：:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...

journal_title：Journal of bacteriology

authors： Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RH

更新日期：2001-10-01 00:00:00

abstract：:After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

journal_title：Journal of bacteriology

authors： Friedman SM,Axel R,Weinstein IB

更新日期：1967-05-01 00:00:00