Abstract:
:The type III secretion system (T3SS) of Pseudomonas aeruginosa is an important virulence factor. The T3SS of P. aeruginosa can be induced by a low calcium signal or upon direct contact with the host cells. The exact pathway of signal sensing and T3SS activation is not clear. By screening a transposon insertion mutant library of the PAK strain, mutation in the mucA gene was found to cause repression of T3SS expression under both type III-inducing and -noninducing conditions. Mutation in the mucA gene is known to cause alginate overproduction, resulting in a mucoid phenotype. Alginate production responds to various environmental stresses and plays a protective role for P. aeruginosa. Comparison of global gene expression of mucA mutant and wild-type PAK under T3SS-inducing conditions confirmed the down regulation of T3SS genes and up regulation of genes involved in alginate biosynthesis. Further analysis indicated that the repression of T3SS in the mucA mutant was AlgU and AlgR dependent, as double mutants mucA/algU and mucA/algR showed normal type III expression. An algR::Gm mutant showed a higher level of type III expression, while overexpression of the algR gene inhibited type III gene expression; thus, it seems that the AlgR-regulated product inhibits the expression of the T3SS genes. It is likely that P. aeruginosa has evolved tight regulatory networks to turn off the energy-expensive T3SS when striving for survival under environmental stresses.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Wu W,Badrane H,Arora S,Baker HV,Jin Sdoi
10.1128/JB.186.22.7575-7585.2004keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
7575-85issue
22eissn
0021-9193issn
1098-5530pii
186/22/7575journal_volume
186pub_type
杂志文章abstract::Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.237-244.1967
更新日期:1967-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.106.2.551-560.1971
更新日期:1971-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4637-4639.2000
更新日期:2000-08-01 00:00:00
abstract::DNA from the STA1 (extracellular glucoamylase) gene of Saccharomyces diastaticus was used as a probe to enable the cloning by colony hybridization of three DNA fragments from Saccharomyces cerevisiae; these were designated S1, S2, and SGA (intracellular, sporulation-specific glucoamylase gene). To examine the evolutio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2142-2149.1987
更新日期:1987-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01418-13
更新日期:2014-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.347-352.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.192-195.1967
更新日期:1967-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.683-690.1996
更新日期:1996-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1067-1074.1974
更新日期:1974-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00420-15
更新日期:2015-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.486-494.1979
更新日期:1979-08-01 00:00:00
abstract::The Gram-positive bacterium Streptococcus pyogenes (also called group A Streptococcus [GAS]), is found strictly in humans and is capable of causing a wide variety of infections. Here we demonstrate that serine catabolism in GAS is controlled by the transcriptional regulator Spy49_0126c. We have designated this regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01516-10
更新日期:2011-04-01 00:00:00
abstract::Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homolo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.1109-1114.1984
更新日期:1984-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.10.3164-3170.1997
更新日期:1997-05-01 00:00:00
abstract::Unlike the great majority of the aerobactin-producing enteric bacteria documented in the literature, Enterobacter cloacae EK33, isolated from a case of human neonatal meningitis, did not show any homology at the DNA level with the prototype aerobactin system encoded by the ColV-K30 plasmid. However, both the nuclear m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5539-5544.1988
更新日期:1988-12-01 00:00:00
abstract::This is the first complete genome sequence of the Enterobacter aerogenes species. Here we present the genome sequence of E. aerogenes KCTC 2190, which contains 5,280,350 bp with a G + C content of 54.8 mol%, 4,912 protein-coding genes, and 109 structural RNAs. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00028-12
更新日期:2012-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.5.1320-1329.2004
更新日期:2004-03-01 00:00:00
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更新日期:2015-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2211-2215.1996
更新日期:1996-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00314-20
更新日期:2020-11-19 00:00:00
abstract::In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purified mutant enzymes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.9.2602-2605.1995
更新日期:1995-05-01 00:00:00
abstract::In Pseudomonas aeruginosa, the CbrA/CbrB two-component system is instrumental in the maintenance of the carbon-nitrogen balance and for growth on carbon sources that are energetically less favorable than the preferred dicarboxylate substrates. The CbrA/CbrB system drives the expression of the small RNA CrcZ, which ant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-06-01 00:00:00
abstract::The genes coding for two structurally different isocitrate dehydrogenase isozymes (IDH-I and IDH-II) of a psychrophilic bacterium, Vibrio sp. strain ABE-1, were cloned and sequenced. Open reading frames of the genes (icdI and icdII) are 1,248 and 2,229 bp in length, respectively. The amino acid sequences predicted fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.21.6873-6880.1993
更新日期:1993-11-01 00:00:00
abstract:UNLABELLED:Current knowledge regarding the mechanism that governs flagellar motor rotation in response to environmental stimuli stems mainly from the study of monotrichous and peritrichous bacteria. Little is known about how two polar flagella, one at each cell pole of the so-called amphitrichous bacterium, are coordin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00172-15
更新日期:2015-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4406-4410.1988
更新日期:1988-09-01 00:00:00
abstract::Chlamydia spp. exhibit a unique biphasic developmental cycle whereby infectious elementary bodies (EBs) invade host epithelial cells and differentiate into noninfectious, metabolically active reticulate bodies (RBs). EBs posses a unique outer envelope where rigidity is achieved by disulfide bonding among cysteine-rich...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05163-11
更新日期:2011-12-01 00:00:00
abstract::The strict human pathogen Neisseria gonorrhoeae is exposed to oxidative damage during infection. N. gonorrhoeae has many defenses that have been demonstrated to counteract oxidative damage. However, recN is the only DNA repair and recombination gene upregulated in response to hydrogen peroxide (H(2)O(2)) by microarray...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00801-06
更新日期:2006-11-01 00:00:00
abstract::Dworkin, Martin (Indiana University Medical Center, Indianapolis, Ind.). Nutritional requirements for vegetative growth of Myxococcus xanthus. J. Bacteriol. 84:250-257. 1962.-This investigation was part of a program to clarify the environmental regulation of fruiting-body formation in a fruiting myxobacterium. By use ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.250-257.1962
更新日期:1962-08-01 00:00:00