Abstract:
:DNA from the STA1 (extracellular glucoamylase) gene of Saccharomyces diastaticus was used as a probe to enable the cloning by colony hybridization of three DNA fragments from Saccharomyces cerevisiae; these were designated S1, S2, and SGA (intracellular, sporulation-specific glucoamylase gene). To examine the evolutionary relationship among these sequences at the nucleotide level, we sequenced S2, S1, SGA and compared them with STA1. These data and RNA blot analysis revealed that the following regions of STA1 were highly conserved in S2, S1, and SGA: upstream regulatory sequences responsible for transcription, a signal sequence for protein secretion, a threonine- and serine-rich domain, and a catalytic domain for glucoamylase activity. These results suggest that an ancestral STA gene was generated relatively recently in an evolutionary time scale by the sequential fusions of S2, S1, and SGA, with S1 functioning as a connector for S2 and SGA. We describe a model for the involvement of short nucleotide sequences flanking the junctions in the gene fusions.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Yamashita I,Nakamura M,Fukui Sdoi
10.1128/jb.169.5.2142-2149.1987subject
Has Abstractpub_date
1987-05-01 00:00:00pages
2142-9issue
5eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::Investigation of the chromosomal region downstream of the lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus revealed the presence of a gene (prtB) encoding a proteinase of 1,946 residues with a predicted molecular mass of 212 kDa. The deduced amino acid sequence showed that PrtB proteinase displays significan...
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1982-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.174.6.1862-1868.1992
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1120-1126.1987
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pub_type: 杂志文章
doi:10.1128/JB.100.3.1242-1247.1969
更新日期:1969-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.94.3.557-561.1967
更新日期:1967-09-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00439-07
更新日期:2007-10-01 00:00:00
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更新日期:1975-05-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2018-01-10 00:00:00